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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000745
  • pan locus tag?: SAUPAN002705000
  • symbol: JSNZ_000745
  • pan gene symbol?: gapR
  • synonym:
  • product: sugar-binding transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000745
  • symbol: JSNZ_000745
  • product: sugar-binding transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 783457..784470
  • length: 1014
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGAAAGACTTATTGCAAGCACAGCAAAAGCTTATACCGGATCTCATAGATAAAATGTAT
    AAACGTTTTTCTATTCTTACTACTATCTCAAAAAATCAGCCTGTCGGACGTCGAAGTTTA
    AGTGAACATATGGATATGACTGAACGTGTACTGCGTTCTGAAACAGATATGCTTAAGAAA
    CAAGATTTGATAAAAGTTAAGCCTACCGGAATGGAAATTACAGCTGAAGGTGAGCAACTG
    ATTTCGCAATTGAAAGGTTACTTTGATATCTATGCAGATGACAATCGTCTGTCAGAAGGT
    ATTAAGAATAAATTTCAAATTAAGGAAGTTCATGTTGTTCCTGGTGATGCTGATAATAGT
    CAATCTGTTAAAACAGAATTAGGTAGACAAGCAGGTCAATTACTTGAAGGCATATTACAA
    GAAGACGCGATAGTTGCTGTAACTGGCGGATCCACGATGGCGTGTGTTAGTGAAGCAATT
    CATTTATTACCATATAATGTATTCTTCGTACCAGCTAGAGGTGGACTAGGCGAAAATGTT
    GTCTTTCAGGCAAACACTATTGCAGCCAGTATGGCACAACAAGCTGGCGGTTATTATACG
    ACGATGTATGTACCTGATAATGTCAGTGAAACAACATATAACACATTGTTGTTAGAGCCA
    TCAGTCATAAACACTTTAGACAAAATTAAACAAGCAAACGTTATATTACACGGCATTGGT
    GATGCGCTGAAGATGGCGCATCGACGTCAATCACCTGAAAAGGTCATTGAACAACTTCAA
    CATCATCAAGCTGTCGGAGAGGCATTTGGTTATTATTTTGATACACAAGGTCAAATTGTC
    CATAAGGTTAAAACAATTGGACTTCAATTAGAAGACCTTGAATCAAAAGACTTTATTTTT
    GCAGTTGCAGGAGGCAAATCGAAAGGTGAAGCAATTAAAGCATACTTGACGATTGCACCC
    AAGAATACAGTGTTAATCACTGATGAAGCCGCAGCAAAGATAATACTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1014

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000745
  • symbol: JSNZ_000745
  • description: sugar-binding transcriptional regulator
  • length: 337
  • theoretical pI: 6.11378
  • theoretical MW: 37210.4
  • GRAVY: -0.196736

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD family protein (TIGR04548; HMM-score: 16.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Sugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 263.5)
    and 4 more
    HTH (CL0123) CggR_N; CggR N-terminal DNA binding domain (PF21715; HMM-score: 94.9)
    PH0730-like_N; PH0730-like, N-terminal domain (PF22167; HMM-score: 18.8)
    HTH_45; Winged helix-turn-helix (PF14947; HMM-score: 16.9)
    MotA_activ; Transcription factor MotA, activation domain (PF09114; HMM-score: 15.9)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9945
    • Cytoplasmic Membrane Score: 0.005
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0004
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002249
    • TAT(Tat/SPI): 0.000242
    • LIPO(Sec/SPII): 0.000544
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHMDMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIKNKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAIHLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLLEPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQGQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKIILE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: GapR* (repression) regulon, SigB (activation) regulon
    GapR*(TF)important in Glycolysis;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  regulation predicted or transferred from N315 and NCTC 8325  [3] [4] [5]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)
  3. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  4. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)
  5. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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