From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0758 [new locus tag: SAUSA300_RS04090 ]
  • pan locus tag?: SAUPAN002708000
  • symbol: tpiA
  • pan gene symbol?: tpiA
  • synonym:
  • product: triosephosphate isomerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0758 [new locus tag: SAUSA300_RS04090 ]
  • symbol: tpiA
  • product: triosephosphate isomerase
  • replicon: chromosome
  • strand: +
  • coordinates: 845808..846569
  • length: 762
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAGAACACCAATTATAGCTGGTAACTGGAAAATGAACAAAACAGTACAAGAAGCAAAA
    GACTTCGTCAATGCATTACCAACATTACCAGATTCAAAAGAAGTAGAATCAGTAATTTGT
    GCACCAGCAATTCAATTAGATGCATTAACTACTGCAGTTAAAGAAGGAAAAGCACAAGGT
    TTAGAAATCGGTGCTCAAAATACGTATTTCGAAGATAATGGTGCGTTCACAGGTGAAACG
    TCTCCAGTTGCATTAGCAGATTTAGGCGTTAAATACGTTGTTATCGGTCATTCTGAACGT
    CGTGAATTATTCCACGAAACAGATGAAGAAATTAACAAAAAAGCGCACGCTATTTTCAAA
    CATGGAATGACTCCAATTATATGTGTTGGTGAAACAGACGAAGAGCGTGAAAGTGGTAAA
    GCTAACGATGTTGTAGGTGAGCAAGTTAAGAAAGCTGTTGCAGGTTTATCTGAAGATCAA
    CTTAAATCAGTTGTAATTGCTTATGAACCAATCTGGGCAATCGGAACTGGTAAATCATCA
    ACATCTGAAGATGCAAATGAAATGTGTGCATTTGTACGTCAAACTATTGCTGACTTATCA
    AGCAAAGAAGTATCAGAAGCAACTCGTATTCAATATGGTGGTAGTGTTAAACCTAACAAC
    ATTAAAGAATACATGGCACAAACTGATATTGATGGGGCATTAGTAGGTGGCGCATCACTT
    AAAGTTGAAGATTTCGTACAATTGTTAGAAGGTGCAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    762

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0758 [new locus tag: SAUSA300_RS04090 ]
  • symbol: TpiA
  • description: triosephosphate isomerase
  • length: 253
  • theoretical pI: 4.5163
  • theoretical MW: 27261.5
  • GRAVY: -0.274308

⊟Function[edit | edit source]

  • reaction:
    EC 5.3.1.1?  ExPASy
    Triose-phosphate isomerase D-glyceraldehyde 3-phosphate = glycerone phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis triose-phosphate isomerase (TIGR00419; EC 5.3.1.1; HMM-score: 197.4)
  • TheSEED  :
    • Triosephosphate isomerase (EC 5.3.1.1)
    Carbohydrates Central carbohydrate metabolism Dihydroxyacetone kinases  Triosephosphate isomerase (EC 5.3.1.1)
    and 1 more
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  Triosephosphate isomerase (EC 5.3.1.1)
  • PFAM:
    TIM_barrel (CL0036) TIM; Triosephosphate isomerase (PF00121; HMM-score: 317.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014787
    • TAT(Tat/SPI): 0.000808
    • LIPO(Sec/SPII): 0.001548
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAIQLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRELFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQLKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKPNNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: GapR* (repression) regulon
    GapR*(TF)important in Glycolysis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_0758&oldid=75999"