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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS10250 [old locus tag: SACOL1960 ]
- pan locus tag?: SAUPAN004926000
- symbol: SACOL_RS10250
- pan gene symbol?: gatB
- synonym:
- product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS10250 [old locus tag: SACOL1960 ]
- symbol: SACOL_RS10250
- product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
- replicon: chromosome
- strand: -
- coordinates: 2020284..2021711
- length: 1428
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1381ATGCATTTTGAAACAGTTATAGGACTTGAAGTTCACGTAGAGTTAAAAACGGACTCAAAA
ATGTTTTCTCCATCACCAGCGCATTTTGGAGCAGAACCTAACTCAAATACAAATGTTATC
GACTTAGCATATCCAGGTGTCTTACCAGTTGTTAATAAGCGTGCAGTAGACTGGGCAATG
CGTGCTGCAATGGCACTAAATATGGAAATCGCAACAGAATCTAAGTTTGACCGTAAGAAC
TATTTCTATCCAGATAATCCAAAAGCATATCAAATTTCTCAATTTGATCAACCAATTGGT
GAAAATGGATATATCGATATCGAAGTCGACGGTGAAACAAAACGAATCGGTATTACTCGT
CTTCACATGGAAGAAGATGCTGGTAAGTCAACACATAAAGGTGAGTATTCATTAGTTGAC
TTGAACCGTCAAGGTACACCGCTAATTGAAATCGTATCTGAACCAGATATTCGTTCACCT
AAAGAAGCATATGCATATTTAGAAAAATTGCGTTCAATTATTCAATACACTGGTGTATCA
GACGTTAAGATGGAAGAGGGATCTTTACGTTGTGATGCTAACATCTCTTTACGTCCATAT
GGTCAAGAAAAATTTGGTACTAAAGCCGAATTGAAAAACTTAAACTCATTTAACTATGTA
CGTAAAGGTTTAGAATATGAAGAAAAACGCCAAGAAGAAGAATTGTTAAATGGTGGAGAA
ATCGGACAAGAAACACGTCGATTTGATGAATCTACAGGTAAAACAATTTTAATGCGTGTT
AAAGAAGGTTCTGATGATTACCGTTACTTCCCAGAGCCTGACATTGTACCTTTATATATT
GATGATGCTTGGAAAGAGCGTGTTCGTCAGACAATTCCTGAATTACCAGATGAACGTAAA
GCTAAGTATGTAAATGAATTAGGTTTACCTGCATACGATGCACACGTATTAACATTGACT
AAAGAAATGTCAGATTTCTTTGAATCAACAATTGAACACGGTGCAGATGTTAAATTAACA
TCTAACTGGTTAATGGGTGGCGTAAACGAATATTTAAATAAAAATCAAGTAGAATTATTA
GATACTAAATTAACACCAGAAAATTTAGCAGGTATGATTAAACTTATCGAAGACGGAACA
ATGAGCAGTAAAATTGCGAAGAAAGTCTTCCCAGAGTTAGCAGCTAAAGGTGGTAATGCT
AAACAGATTATGGAAGATAATGGCTTAGTTCAAATTTCTGATGAAGCAACACTTCTAAAA
TTTGTAAATGAAGCATTAGACAATAACGAACAATCAGTTGAAGATTACAAAAATGGTAAA
GGCAAAGCTATGGGCTTCTTAGTTGGTCAAATTATGAAAGCGTCTAAAGGTCAAGCTAAT
CCACAATTAGTAAATCAACTATTAAAACAAGAATTAGATAAAAGATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS10250 [old locus tag: SACOL1960 ]
- symbol: SACOL_RS10250
- description: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
- length: 475
- theoretical pI: 4.77547
- theoretical MW: 53656.3
- GRAVY: -0.602947
⊟Function[edit | edit source]
- reaction: EC 6.3.5.-? ExPASyATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate? ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate?
- TIGRFAM: Protein synthesis tRNA aminoacylation aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (TIGR00133; EC 6.3.5.-; HMM-score: 595.1)and 1 moreProtein synthesis tRNA aminoacylation glutamyl-tRNA(Gln) amidotransferase, subunit E (TIGR00134; EC 6.3.5.-; HMM-score: 140.6)
- TheSEED: see SACOL1960
- PFAM: GCS (CL0286) GatB_N; GatB/GatE catalytic domain (PF02934; HMM-score: 375)and 3 moreGatB_YqeY (CL0279) GatB_Yqey; GatB domain (PF02637; HMM-score: 182.8)no clan defined MOZART1; Mitotic-spindle organizing gamma-tubulin ring associated (PF12554; HMM-score: 15.4)GatB_YqeY (CL0279) YqeY; Yqey-like protein (PF09424; HMM-score: 13.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.008143
- TAT(Tat/SPI): 0.000704
- LIPO(Sec/SPII): 0.001478
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSPKEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQELDKR
⊟Experimental data[edit | edit source]
- experimentally validated: see SACOL1960
- protein localization: see SACOL1960
- quantitative data / protein copy number per cell: see SACOL1960
- interaction partners:
SACOL_RS10255 (gatA) glutamyl-tRNA(Gln) amidotransferase subunit A [1] (data from MRSA252) SACOL_RS03080 elongation factor Tu [1] (data from MRSA252) SACOL_RS06505 30S ribosomal protein S2 [1] (data from MRSA252) SACOL_RS08930 pyruvate kinase [1] (data from MRSA252) SACOL_RS08995 universal stress protein UspA [1] (data from MRSA252) SACOL_RS11695 30S ribosomal protein S5 [1] (data from MRSA252) SACOL_RS11750 30S ribosomal protein S3 [1] (data from MRSA252) SACOL_RS12085 hypothetical protein [1] (data from MRSA252) SACOL_RS13365 pyruvate oxidase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)