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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00442
  • pan locus tag?: SAUPAN002198000
  • symbol: SAOUHSC_00442
  • pan gene symbol?: dnaX
  • synonym:
  • product: DNA polymerase III subunits gamma and tau

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00442
  • symbol: SAOUHSC_00442
  • product: DNA polymerase III subunits gamma and tau
  • replicon: chromosome
  • strand: +
  • coordinates: 445284..446957
  • length: 1674
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTACAGACCCCAAAGTTTCGAGGATGTCGTCGGACAAGAACATGTCACGAAGACATTG
    CGCAATGCGATTTCGAAAGAAAAACAGTCGCATGCTTATATTTTTAGTGGTCCGAGAGGT
    ACGGGGAAAACGAGTATTGCCAAAGTGTTTGCTAAAGCAATCAACTGTCTAAATAGCACT
    GATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCA
    GATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGA
    GACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTG
    CACATGCTAACAACAGGTGCTTTTAATGCCCTTTTAAAGACGTTAGAAGAACCTCCAGCA
    CACGCTATTTTTATATTGGCAACGACAGAACCACATAAAATCCCTCCAACAATCATTTCT
    AGGGCACAACGTTTTGATTTTAAAGCAATTAGCCTAGATCAAATTGTTGAACGTTTAAAA
    TTTGTAGCAGATGCACAACAAATTGAATGTGAAGATGAAGCCTTGGCATTTATCGCTAAA
    GCGTCTGAAGGGGGTATGCGTGATGCATTAAGTATTATGGATCAGGCTATTGCATTTGGT
    GATGGTACGTTAACATTGCAAGATGCGTTGAATGTCACAGGTAGCGTACATGATGAAGCG
    TTGGATCACTTGTTTGATGATATTGTACAAGGTGACGTACAAGCATCTTTTAAAAAATAC
    CATCAGTTTATAACAGAAGGTAAAGAAGTGAATCGCCTAATAAATGATATGATTTATTTT
    GTCAGAGATACGATTATGAATAAAACATCTGAGAAAGATACTGAGTATCGAGCACTGATG
    AACTTAGAATTAGATATGTTATATCAAATGATTGATCTTATTAATGATACATTAGTGTCG
    ATTCGTTTTAGTGTGAATCAAAACGTTCATTTTGAAGTGTTGTTAGTAAAATTAGCTGAG
    CAGATTAAGGGTCAACCACAAGTGATTGCGAATGTAGCTGAACCAGCACAAATTGCTTCA
    TCGCCAAACACAGATGTATTGTTGCAACGTATGGAACAGTTAGAGCAAGAACTAAAAACA
    CTAAAAGCACAAGGAGTGAGTGTCGCTCCTGTTCAAAAATCTTCGAAAAAGCCTGCGAGA
    GGCATACAAAAATCTAAAAATGCATTTTCAATGCAACAAATTGCAAAAGTGCTAGATAAA
    GCGAATAAGGCAGATATCAAATTGTTGAAAGATCATTGGCAAGAAGTGATTGATCATGCC
    AAAAATAATGATAAAAAATCACTCGTTAGTTTATTGCAAAATTCGGAACCTGTGGCGGCA
    AGTGAAGATCACGTACTTGTGAAATTTGAGGAAGAGATCCATTGTGAAATCGTCAATAAA
    GACGACGAGAAACGTAGTAGTATAGAAAGTGTTGTATGTAATATCGTTAATAAAAACGTT
    AAAGTTGTTGGTGTACCATCAGATCAATGGCAAAGAGTTCGAACGGAATATTTACAAAAT
    CGTAAAAACGAAGGCGATGATATGCCAAAGCAACAAGCACAACAAACAGATATTGCTCAA
    AAAGCAAAAGATCTTTTCGGTGAAGAAACTGTACATGTGATAGATGAAGAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1674

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00442
  • symbol: SAOUHSC_00442
  • description: DNA polymerase III subunits gamma and tau
  • length: 557
  • theoretical pI: 5.24004
  • theoretical MW: 62458.4
  • GRAVY: -0.412388

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 452.2)
    and 10 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 168.3)
    Cellular processes Cellular processes Sporulation and germination ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 39.3)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 39.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 35.1)
    Cellular processes Cellular processes Sporulation and germination ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 29.4)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 29.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta subunit (TIGR01128; EC 2.7.7.7; HMM-score: 24.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion AAA-ATPase EccA (TIGR03922; HMM-score: 22)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 16.4)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein K (TIGR02881; HMM-score: 15.6)
  • TheSEED  :
    • DNA polymerase III subunits gamma and tau (EC 2.7.7.7)
    DNA Metabolism DNA replication DNA-replication  DNA polymerase III subunits gamma and tau (EC 2.7.7.7)
    and 1 more
    DNA Metabolism DNA uptake, competence DNA processing cluster  DNA polymerase III subunits gamma and tau (EC 2.7.7.7)
  • PFAM:
    P-loop_NTPase (CL0023) DNA_pol3_delta2; DNA polymerase III, delta subunit (PF13177; HMM-score: 161.9)
    and 20 more
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 54.2)
    RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 32.3)
    TIP49; TIP49 C-terminus (PF06068; HMM-score: 28.6)
    post-AAA (CL0604) DNA_pol3_gamma3; DNA polymerase III subunits gamma and tau domain III (PF12169; HMM-score: 24.4)
    P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 24.3)
    AAA_30; AAA domain (PF13604; HMM-score: 22.9)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 22.5)
    AAA_19; AAA domain (PF13245; HMM-score: 20.6)
    PIF1; PIF1-like helicase (PF05970; HMM-score: 20.4)
    AAA_14; AAA domain (PF13173; HMM-score: 20.1)
    AAA_22; AAA domain (PF13401; HMM-score: 20.1)
    Mg_chelatase; Magnesium chelatase, subunit ChlI (PF01078; HMM-score: 18.8)
    AAA_18; AAA domain (PF13238; HMM-score: 18.4)
    TniB; Bacterial TniB protein (PF05621; HMM-score: 17.7)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 17.5)
    no clan defined AAA_assoc_2; AAA C-terminal domain (PF16193; HMM-score: 17.1)
    P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 16.9)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 14)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 12.7)
    no clan defined Com_YlbF; Control of competence regulator ComK, YlbF/YmcA (PF06133; HMM-score: 7.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.140965
    • TAT(Tat/SPI): 0.016577
    • LIPO(Sec/SPII): 0.00438
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFSGPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVDEIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPHKIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDALSIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGKEVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVNQNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQGVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKNNDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNVKVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.000 4.001 4.002 4.003 4.004 4.005 4.006 4.007 4.008 4.009 4.010 4.011 4.012 4.013 4.014 4.015 4.016 4.017 4.018 4.019 4.020 4.021 4.022 4.023 4.024 4.025 4.026 4.027 4.028 4.029 4.030 4.031 4.032 4.033 4.034 4.035 4.036 4.037 4.038 4.039 4.040 4.041 4.042 4.043 4.044 4.045 4.046 4.047 4.048 4.049 4.050 4.051 4.052 4.053 4.054 4.055 4.056 4.057 4.058 4.059 4.060 4.061 4.062 4.063 4.064 4.065 4.066 4.067 4.068 4.069 4.070 4.071 4.072 4.073 4.074 4.075 4.076 4.077 4.078 4.079 4.080 4.081 4.082 4.083 4.084 4.085 4.086 4.087 4.088 4.089 4.090 4.091 4.092 4.093 4.094 4.095 4.096 4.097 4.098 4.099 4.100 4.101 4.102 4.103 4.104 4.105 4.106 4.107 4.108 4.109 4.110 4.111 4.112 4.113 4.114 4.115 4.116 4.117 4.118 4.119 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  5. ↑ 5.0 5.1 5.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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