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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1674 [new locus tag: SAUSA300_RS09140 ]
  • pan locus tag?: SAUPAN004387000
  • symbol: SAUSA300_1674
  • pan gene symbol?: htrA1
  • synonym:
  • product: putative serine protease HtrA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1674 [new locus tag: SAUSA300_RS09140 ]
  • symbol: SAUSA300_1674
  • product: putative serine protease HtrA
  • replicon: chromosome
  • strand: +
  • coordinates: 1840096..1841370
  • length: 1275
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTCAGATTTTAATCATACAGATCATTCTACAACAAACCATAGCCAAACACCTAGATAC
    AGAAGACCTAAATTTCCATGGTTTAAAACAGTCATCGTTGCATTGATTGCTGGAATTATT
    GGTGCACTTCTAGTACTTGGTATAGGCAAAGTATTAAATAGTACAATTTTAAATAAAGAT
    GGTTCAACTGTTCAGACAACAAATAATAAAGGTGGCAATCAATTAGACGGTCAAAGCAAG
    AAATTCGGTACCGTTCATGAAATGATAAAATCTGTCTCCCCTACAATTGTTGGAGTTATT
    AACATGCAAAAAGCATCAAGTGTAGACGACTTATTAAAAGGCAAATCATCTAAACCATCT
    GAAGCTGGAGTAGGTTCAGGTGTTATCTATCAAATAAACAACAATTCAGCTTATATCGTT
    ACAAACAATCATGTTATTGATGGCGCAAATGAAATTAGAGTCCAATTACATAATAAAAAA
    CAAGTTAAAGCGAAATTAGTTGGTAAAGATGCAGTAACTGATATTGCTGTACTTAAAATT
    GAAAATACAAAAGGTATTAAAGCGATTCAATTTGCCAACTCTTCAAAAGTACAAACTGGC
    GATAGCGTATTCGCAATGGGTAACCCATTAGGATTACAATTTGCTAACTCTGTAACATCT
    GGTATCATTTCAGCAAGCGAACGTACGATTGACGCTGAGACAACTGGTGGCAATACAAAA
    GTTAGCGTTCTTCAAACAGATGCTGCTATTAACCCAGGTAACTCAGGTGGCGCATTAGTA
    GATATTAATGGTAATTTAGTTGGTATTAACTCAATGAAAATTGCTGCGACACAAGTTGAA
    GGTATCGGGTTTGCTATTCCAAGTAATGAAGTTAAAGTAACAATTGAACAACTTGTAAAA
    CATGGTAAAATTGACCGCCCTTCGATTGGTATTGGTTTAATTAATTTGAAAGATATTCCT
    GAAGAAGAGCGCGAGCAACTTCATACTGATAGAGAAGACGGTATTTATGTCGCCAAAGCT
    GATAGTGATATTGATCTTAAAAAAGGTGATATTATTACAGAAATTGATGGCAAGAAAATT
    AAAGATGATGTTGATTTAAGAAGCTATTTATATGAAAATAAAAAACCTGGTGAATCAGTC
    ACTGTTACCGTTATCCGTGATGGTAAAACAAAAGAAGTTAAAGTGAAATTAAAACAACAA
    AAAGAACAACCAAAACGTCAAAGCCGATCAGAACGTCAATCACCTGGCCAAGGCGATAGA
    GATTTCTTTAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1275

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1674 [new locus tag: SAUSA300_RS09140 ]
  • symbol: SAUSA300_1674
  • description: putative serine protease HtrA
  • length: 424
  • theoretical pI: 9.97226
  • theoretical MW: 45802.6
  • GRAVY: -0.416981

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 291.6)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 291.6)
    and 4 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 182.6)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 182.6)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides RIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 19)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein C (TIGR01713; HMM-score: 16.6)
  • TheSEED  :
    • Serine protease Do-like HtrA (EC 3.4.21.107)
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 109.3)
    and 8 more
    Trypsin; Trypsin (PF00089; HMM-score: 59.8)
    PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 57.6)
    Tricorn_PDZ; Tricorn protease PDZ domain (PF14685; HMM-score: 22)
    Peptidase_PA (CL0124) Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 17.3)
    PDZ-like (CL0466) PDZ; PDZ domain (Also known as DHR or GLGF) (PF00595; HMM-score: 15.6)
    Peptidase_PA (CL0124) DUF31; Putative peptidase (DUF31) (PF01732; HMM-score: 14)
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 11.9)
    Peptidase_PA (CL0124) Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 4.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.023768
    • TAT(Tat/SPI): 0.001027
    • LIPO(Sec/SPII): 0.005047
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALLVLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINMQKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKKQVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSVTSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAATQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDGIYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKEVKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 1.57 1.58 1.59 1.60 1.61 1.62 1.63 1.64 1.65 1.66 1.67 1.68 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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