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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2125 [new locus tag: SAUSA300_RS11705 ]
  • pan locus tag?: SAUPAN005553000
  • symbol: SAUSA300_2125
  • pan gene symbol?: salA
  • synonym:
  • product: ATP-binding Mrp/Nbp35 family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2125 [new locus tag: SAUSA300_RS11705 ]
  • symbol: SAUSA300_2125
  • product: ATP-binding Mrp/Nbp35 family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2298031..2299035
  • length: 1005
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGCCTTTAAAAGAAACAGAAGGTATTGTTGAAGTTTCTATTAAGGAAGAAAAAGAACAT
    GTGAGTGTTAAATTAGCAATGGCACAATTAGGTGGTGCACCGCAATTAGATTTACAGATG
    GCTGTTGTTAATGCATTAAAAGAAAACGGTGCGAAAACGGTCGGTATACGATTTGAAACA
    TTGCCGGAAGAAAAAGTAAATCAATTTAAACCAAAAGAAGAAAATAAACCTAAAACGATA
    GAAGGCCTATTATCTCAAAATAATCCAGTCGAATTTATTGCAATAGCCTCCGGTAAAGGT
    GGTGTCGGTAAATCTACTGTTGCAGTAAATTTAGCCGTTGCCTTAGCTCGTGAAGGGAAA
    AAAGTCGGATTAGTAGATGCCGATATATATGGATTTAGTGTACCAGATATGATGGGTATT
    GATGAAAAGCCTGGAATTAAAGGGAAGGAAGTAATTCCAGTTGAACGTCATGGCGTTAAA
    GTTATATCAATGGCCTTTTTTGTGGAAGAAAATGCGCCAGTTATATGGAGAGGGCCAATG
    TTAGGTAAAATGTTGACGAATTTCTTTACAGAAGTTAAATGGGGAGACATTGAATATTTA
    ATACTCGATCTTCCACCTGGAACAGGAGATGTAGCTTTAGATGTTCATACGATGTTACCT
    TCAAGTAAGGAAATTATTGTAACGACACCTCATCCTACAGCAGCATTTGTTGCAGCTCGC
    GCAGGTGCGATGGCAAAACATACGGATCATTCTATTCTTGGCGTAATTGAAAACATGAGT
    TATTTTGAAAGTAAAGAGACGGGTAATAAAGAATATGTCTTTGGCAAAGGTGGCGGTACT
    AAGTTAGCTGATGAACTTAATACTCAATTACTTGGGGAATTACCTTTAGAGCAACCATCT
    TGGAATCCAAAAGATTTTGCACCGTCAATATATCAATCAGATGATCGTCTAGGTAAAATT
    TATAGCTCGATTGCACAAAAAGTTATAGCTGCTACAAATAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2125 [new locus tag: SAUSA300_RS11705 ]
  • symbol: SAUSA300_2125
  • description: ATP-binding Mrp/Nbp35 family protein
  • length: 334
  • theoretical pI: 5.49057
  • theoretical MW: 36152.5
  • GRAVY: -0.148204

⊟Function[edit | edit source]

  • TIGRFAM:
    cell division ATPase MinD (TIGR01969; HMM-score: 70)
    and 20 more
    Cellular processes Cellular processes Cell division septum site-determining protein MinD (TIGR01968; HMM-score: 51.3)
    helicase/secretion neighborhood CpaE-like protein (TIGR03815; HMM-score: 45.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids capsular exopolysaccharide family (TIGR01007; HMM-score: 37)
    exopolysaccharide/PEP-CTERM locus tyrosine autokinase (TIGR03018; EC 2.7.10.2; HMM-score: 35.4)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides cellulose synthase operon protein YhjQ (TIGR03371; HMM-score: 30.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll light-independent protochlorophyllide reductase, iron-sulfur ATP-binding protein (TIGR01281; EC 1.3.7.7; HMM-score: 27.9)
    arsenical pump-driving ATPase (TIGR04291; EC 3.6.1.-; HMM-score: 27.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids exopolysaccharide transport protein family (TIGR01005; HMM-score: 25.8)
    Hypothetical proteins Conserved transport-energizing ATPase, TRC40/GET3/ArsA family (TIGR00345; EC 3.6.1.-; HMM-score: 25.7)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogenase iron protein (TIGR01287; EC 1.18.6.1; HMM-score: 24)
    chain length determinant protein tyrosine kinase EpsG (TIGR03029; HMM-score: 21.7)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid partitioning protein RepA (TIGR03453; HMM-score: 18.1)
    DNA repair and recombination protein RadB (TIGR02237; HMM-score: 16.5)
    Metabolism Energy metabolism Photosynthesis chlorophyllide reductase iron protein subunit X (TIGR02016; HMM-score: 16.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 15.8)
    Signal transduction Regulatory functions Protein interactions LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 15.8)
    Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 15.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal recognition particle-docking protein FtsY (TIGR00064; HMM-score: 14.1)
    Metabolism Central intermediary metabolism Sulfur metabolism adenylyl-sulfate kinase (TIGR00455; EC 2.7.1.25; HMM-score: 12.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin putative heme d1 biosynthesis radical SAM protein NirJ2 (TIGR04055; EC 1.3.99.-; HMM-score: 12.3)
  • TheSEED  :
    • Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like
  • PFAM:
    P-loop_NTPase (CL0023) ParA; NUBPL iron-transfer P-loop NTPase (PF10609; HMM-score: 289.5)
    and 17 more
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 52.4)
    AAA_31; AAA domain (PF13614; HMM-score: 45.3)
    MipZ; ATPase MipZ (PF09140; HMM-score: 32.2)
    Fer4_NifH; 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family (PF00142; HMM-score: 27.4)
    ArsA_ATPase; Anion-transporting ATPase (PF02374; HMM-score: 27.3)
    AAA_25; AAA domain (PF13481; HMM-score: 21.4)
    VirC1; VirC1 protein (PF07015; HMM-score: 20.4)
    CLP1_P; mRNA cleavage and polyadenylation factor CLP1 P-loop (PF16575; HMM-score: 18.8)
    CBP_BcsQ; Cellulose biosynthesis protein BcsQ (PF06564; HMM-score: 18.3)
    SRP54; SRP54-type protein, GTPase domain (PF00448; HMM-score: 16.4)
    ArgK; ArgK protein (PF03308; HMM-score: 15.2)
    APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 13.5)
    IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 12.7)
    no clan defined PilZ; PilZ domain (PF07238; HMM-score: 12.7)
    P-loop_NTPase (CL0023) KTI12; Chromatin associated protein KTI12 (PF08433; HMM-score: 12.2)
    PhoH; PhoH-like protein (PF02562; HMM-score: 11.9)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010617
    • TAT(Tat/SPI): 0.002248
    • LIPO(Sec/SPII): 0.000647
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPLKETEGIVEVSIKEEKEHVSVKLAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEGLLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGIDEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIEYLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVIENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSDDRLGKIYSSIAQKVIAATNK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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