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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1684 [new locus tag: SAUSA300_RS09205 ]
- pan locus tag?: SAUPAN004405000
- symbol: SAUSA300_1684
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1684 [new locus tag: SAUSA300_RS09205 ]
- symbol: SAUSA300_1684
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 1856424..1857623
- length: 1200
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913798 NCBI
- RefSeq: YP_494378 NCBI
- BioCyc: see SAUSA300_RS09205
- MicrobesOnline: 1293199 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGACGCAGCAACAAAATGATAAAAGAACATTAAAAAATAAACACACTTATCAAAATGAA
CCACTACCAAACCGTAAAGATTTTGTTGTTAGTTTTATAACTGGCGCGCTTGTTGGTTCA
GCTTTAGGTTTGTATTTTAAAAATAAAGTTTATCAAAAAGCAGATGATTTAAAAGTCAAA
GAACAAGAACTGTCGCAAAAGTTTGAAGAAAGAAAAACGCAACTTGAAGAAACGGTTGCC
TATACAAAAGAACGTGTTGAAGGATTTTTAAACAAATCTAAAAATGAGCAAGCGGCATTA
AAGGCACAACAAGCAGCGATAAAAGAAGAAGCAAGTGCAAATAATTTGAGTGATACATCA
CAAGAGGCACAAGAGATTCAAGAAGCTAAAAAAGAAGCACAAGCAGAAACAGATAAAAGT
GCAGCTGTATCAAATGAAGAACCAAAAGCAGTGGCATTGAAAGCACAACAAGCAGCGATA
AAAGAAGAAGCAAGTGCAAATAATTTAAGTGATATATCACAAGAGGCACAAGAGGTTCAA
GAAGCTAAAAAAGAAGCACAAGCAGAGAAAGACAGTGACACATTAACTAAAGATGCAAGT
GCAGCAAAGGTAGAAGTATCAAAACCAGAGTCACAAGCTGAAAGATTAGCAAACGCTGCA
AAACAGAAGCAAGCTAAATTAACACCAGGTTCAAAAGAGAGTCAATTAACTGAAGCGTTA
TTTGCAGAAAAACCAGTTGCTAAAAATGACTTGAAAGAAATTCCTCAATTAGTTACTAAA
AAGAATGATGTATCAGAGACAGAGACGGTTAATATAGATAATAAAGACACTGTTAAACAA
AAAGAAGCTAAATTTGAAAATGGTGTTATTACACGTAAAGCTGATGAAAAAACAACTAAT
AATACAGCTGTTGACAAGAAATCAGGTAAACAATCTAAAAAAACAACACCTTCAAATAAA
CGAAATGCATCAAAAGCATCTACAAATAAAACTTCAGGTCAGAAAAAGCAACATAATAAG
AAATCATCACAAGGTGCAAAGAAACAAAGTAGTTCAAGTAAGTCAACTCAAAAGAATAAT
CAAACTAGTAATAAGAATTCAAAAACAACAAATGCTAAATCATCCAATGCATCAAAAACG
CCAAATGCTAAAGTTGAGAAAGCTAAAAGTAAAATAGAGAAACGTACATTCAATGACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1684 [new locus tag: SAUSA300_RS09205 ]
- symbol: SAUSA300_1684
- description: hypothetical protein
- length: 399
- theoretical pI: 10.2731
- theoretical MW: 43925.4
- GRAVY: -1.25414
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cellwall
- Cytoplasmic Score: 0.01
- Cytoplasmic Membrane Score: 0.11
- Cellwall Score: 9.25
- Extracellular Score: 0.63
- Internal Helices: 0
- LocateP: N-terminally anchored (No CS)
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 5
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.014086
- TAT(Tat/SPI): 0.022843
- LIPO(Sec/SPII): 0.00329
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGLYFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKAQQAAIKEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEASANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTFND
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
SAUSA300_2058 (atpD) F0F1 ATP synthase subunit beta [1] (data from MRSA252) SAUSA300_0760 (eno) phosphopyruvate hydratase [1] (data from MRSA252) SAUSA300_1080 (ftsZ) cell division protein FtsZ [1] (data from MRSA252) SAUSA300_2067 (glyA) serine hydroxymethyltransferase [1] (data from MRSA252) SAUSA300_1362 (hup) DNA-binding protein HU [1] (data from MRSA252) SAUSA300_1627 (infC) translation initiation factor IF-3 [1] (data from MRSA252) SAUSA300_0996 (lpdA) dihydrolipoamide dehydrogenase [1] (data from MRSA252) SAUSA300_0032 (mecA) penicillin-binding protein 2' [1] (data from MRSA252) SAUSA300_1251 (parC) DNA topoisomerase IV subunit A [1] (data from MRSA252) SAUSA300_0993 (pdhA) pyruvate dehydrogenase E1 component, alpha subunit [1] (data from MRSA252) SAUSA300_0523 (rplA) 50S ribosomal protein L1 [1] (data from MRSA252) SAUSA300_2201 (rplB) 50S ribosomal protein L2 [1] (data from MRSA252) SAUSA300_2204 (rplC) 50S ribosomal protein L3 [1] (data from MRSA252) SAUSA300_2203 (rplD) 50S ribosomal protein L4 [1] (data from MRSA252) SAUSA300_2192 (rplE) 50S ribosomal protein L5 [1] (data from MRSA252) SAUSA300_2189 (rplF) 50S ribosomal protein L6 [1] (data from MRSA252) SAUSA300_0524 (rplJ) 50S ribosomal protein L10 [1] (data from MRSA252) SAUSA300_0522 (rplK) 50S ribosomal protein L11 [1] (data from MRSA252) SAUSA300_0525 (rplL) 50S ribosomal protein L7/L12 [1] (data from MRSA252) SAUSA300_2185 (rplO) 50S ribosomal protein L15 [1] (data from MRSA252) SAUSA300_2197 (rplP) 50S ribosomal protein L16 [1] (data from MRSA252) SAUSA300_2177 (rplQ) 50S ribosomal protein L17 [1] (data from MRSA252) SAUSA300_1134 (rplS) 50S ribosomal protein L19 [1] (data from MRSA252) SAUSA300_1603 (rplU) 50S ribosomal protein L21 [1] (data from MRSA252) SAUSA300_2199 (rplV) 50S ribosomal protein L22 [1] (data from MRSA252) SAUSA300_2202 (rplW) 50S ribosomal protein L23 [1] (data from MRSA252) SAUSA300_2074 (rpmE2) 50S ribosomal protein L31 type B [1] (data from MRSA252) SAUSA300_1666 (rpsD) 30S ribosomal protein S4 [1] (data from MRSA252) SAUSA300_2187 (rpsE) 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_0366 (rpsF) 30S ribosomal protein S6 [1] (data from MRSA252) SAUSA300_2171 (rpsI) 30S ribosomal protein S9 [1] (data from MRSA252) SAUSA300_2179 (rpsK) 30S ribosomal protein S11 [1] (data from MRSA252) SAUSA300_0530 (rpsL) 30S ribosomal protein S12 [1] (data from MRSA252) SAUSA300_2195 (rpsQ) 30S ribosomal protein S17 [1] (data from MRSA252) SAUSA300_2200 (rpsS) 30S ribosomal protein S19 [1] (data from MRSA252) SAUSA300_1593 (secF) bifunctional preprotein translocase subunit SecD/SecF [1] (data from MRSA252) SAUSA300_1305 (sucB) dihydrolipoamide succinyltransferase [1] (data from MRSA252) SAUSA300_0307 5'-nucleotidase [1] (data from MRSA252) SAUSA300_0489 putative cell division protein FtsH [1] (data from MRSA252) SAUSA300_0649 hypothetical protein [1] (data from MRSA252) SAUSA300_1083 hypothetical protein [1] (data from MRSA252) SAUSA300_1337 hypothetical protein [1] (data from MRSA252) SAUSA300_1554 hypothetical protein [1] (data from MRSA252) SAUSA300_1652 hypothetical protein [1] (data from MRSA252) SAUSA300_1674 putative serine protease HtrA [1] (data from MRSA252) SAUSA300_1687 FtsK/SpoIIIE family protein [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)