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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS09205 [old locus tag: SAUSA300_1684 ]
- pan locus tag?: SAUPAN004405000
- symbol: SAUSA300_RS09205
- pan gene symbol?: —
- synonym:
- product: membrane protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS09205 [old locus tag: SAUSA300_1684 ]
- symbol: SAUSA300_RS09205
- product: membrane protein
- replicon: chromosome
- strand: -
- coordinates: 1856424..1857623
- length: 1200
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1856424..1857623) NCBI
- BioCyc: SAUSA300_RS09205 BioCyc
- MicrobesOnline: see SAUSA300_1684
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGACGCAGCAACAAAATGATAAAAGAACATTAAAAAATAAACACACTTATCAAAATGAA
CCACTACCAAACCGTAAAGATTTTGTTGTTAGTTTTATAACTGGCGCGCTTGTTGGTTCA
GCTTTAGGTTTGTATTTTAAAAATAAAGTTTATCAAAAAGCAGATGATTTAAAAGTCAAA
GAACAAGAACTGTCGCAAAAGTTTGAAGAAAGAAAAACGCAACTTGAAGAAACGGTTGCC
TATACAAAAGAACGTGTTGAAGGATTTTTAAACAAATCTAAAAATGAGCAAGCGGCATTA
AAGGCACAACAAGCAGCGATAAAAGAAGAAGCAAGTGCAAATAATTTGAGTGATACATCA
CAAGAGGCACAAGAGATTCAAGAAGCTAAAAAAGAAGCACAAGCAGAAACAGATAAAAGT
GCAGCTGTATCAAATGAAGAACCAAAAGCAGTGGCATTGAAAGCACAACAAGCAGCGATA
AAAGAAGAAGCAAGTGCAAATAATTTAAGTGATATATCACAAGAGGCACAAGAGGTTCAA
GAAGCTAAAAAAGAAGCACAAGCAGAGAAAGACAGTGACACATTAACTAAAGATGCAAGT
GCAGCAAAGGTAGAAGTATCAAAACCAGAGTCACAAGCTGAAAGATTAGCAAACGCTGCA
AAACAGAAGCAAGCTAAATTAACACCAGGTTCAAAAGAGAGTCAATTAACTGAAGCGTTA
TTTGCAGAAAAACCAGTTGCTAAAAATGACTTGAAAGAAATTCCTCAATTAGTTACTAAA
AAGAATGATGTATCAGAGACAGAGACGGTTAATATAGATAATAAAGACACTGTTAAACAA
AAAGAAGCTAAATTTGAAAATGGTGTTATTACACGTAAAGCTGATGAAAAAACAACTAAT
AATACAGCTGTTGACAAGAAATCAGGTAAACAATCTAAAAAAACAACACCTTCAAATAAA
CGAAATGCATCAAAAGCATCTACAAATAAAACTTCAGGTCAGAAAAAGCAACATAATAAG
AAATCATCACAAGGTGCAAAGAAACAAAGTAGTTCAAGTAAGTCAACTCAAAAGAATAAT
CAAACTAGTAATAAGAATTCAAAAACAACAAATGCTAAATCATCCAATGCATCAAAAACG
CCAAATGCTAAAGTTGAGAAAGCTAAAAGTAAAATAGAGAAACGTACATTCAATGACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS09205 [old locus tag: SAUSA300_1684 ]
- symbol: SAUSA300_RS09205
- description: membrane protein
- length: 399
- theoretical pI: 10.2731
- theoretical MW: 43925.4
- GRAVY: -1.25414
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Unknown substrate transport protein (TIGR00833; HMM-score: 3.5)
- TheSEED: see SAUSA300_1684
- PFAM: no clan defined Peptidase_M24; Metallopeptidase family M24 (PF00557; HMM-score: 9.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cellwall
- Cytoplasmic Score: 0.01
- Cytoplasmic Membrane Score: 0.11
- Cellwall Score: 9.25
- Extracellular Score: 0.63
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.014086
- TAT(Tat/SPI): 0.022843
- LIPO(Sec/SPII): 0.00329
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
- GI: 446116043 NCBI
- RefSeq: WP_000193898 NCBI
- UniProt: see SAUSA300_1684
⊟Protein sequence[edit | edit source]
- MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGLYFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKAQQAAIKEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEASANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTFND
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
SAUSA300_RS00165 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA [1] (data from MRSA252) SAUSA300_RS01635 5'-nucleotidase, lipoprotein e(P4) family [1] (data from MRSA252) SAUSA300_RS01940 30S ribosomal protein S6 [1] (data from MRSA252) SAUSA300_RS02625 zinc metalloprotease [1] (data from MRSA252) SAUSA300_RS02795 50S ribosomal protein L11 [1] (data from MRSA252) SAUSA300_RS02800 50S ribosomal protein L1 [1] (data from MRSA252) SAUSA300_RS02805 50S ribosomal protein L10 [1] (data from MRSA252) SAUSA300_RS02810 50S ribosomal protein L7/L12 [1] (data from MRSA252) SAUSA300_RS02835 30S ribosomal protein S12 [1] (data from MRSA252) SAUSA300_RS03480 hypothetical protein [1] (data from MRSA252) SAUSA300_RS04100 enolase [1] (data from MRSA252) SAUSA300_RS05350 pyruvate dehydrogenase E1 component subunit alpha [1] (data from MRSA252) SAUSA300_RS05365 dihydrolipoyl dehydrogenase [1] (data from MRSA252) SAUSA300_RS05855 cell division protein FtsZ [1] (data from MRSA252) SAUSA300_RS05870 cell division protein SepF [1] (data from MRSA252) SAUSA300_RS06135 50S ribosomal protein L19 [1] (data from MRSA252) SAUSA300_RS06790 DNA topoisomerase 4 subunit A [1] (data from MRSA252) SAUSA300_RS07100 dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex [1] (data from MRSA252) SAUSA300_RS07290 cell cycle protein GpsB [1] (data from MRSA252) SAUSA300_RS07430 DNA-binding protein HU [1] (data from MRSA252) SAUSA300_RS08470 RNA-binding protein [1] (data from MRSA252) SAUSA300_RS08680 protein translocase subunit SecDF [1] (data from MRSA252) SAUSA300_RS08730 50S ribosomal protein L21 [1] (data from MRSA252) SAUSA300_RS08875 translation initiation factor IF-3 [1] (data from MRSA252) SAUSA300_RS09015 universal stress protein [1] (data from MRSA252) SAUSA300_RS09090 30S ribosomal protein S4 [1] (data from MRSA252) SAUSA300_RS09140 serine protease [1] (data from MRSA252) SAUSA300_RS09220 DNA translocase FtsK [1] (data from MRSA252) SAUSA300_RS11335 ATP synthase subunit beta [1] (data from MRSA252) SAUSA300_RS11385 serine hydroxymethyltransferase [1] (data from MRSA252) SAUSA300_RS11420 50S ribosomal protein L31 type B [1] (data from MRSA252) SAUSA300_RS11985 30S ribosomal protein S9 [1] (data from MRSA252) SAUSA300_RS12015 50S ribosomal protein L17 [1] (data from MRSA252) SAUSA300_RS12025 30S ribosomal protein S11 [1] (data from MRSA252) SAUSA300_RS12055 50S ribosomal protein L15 [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS12075 50S ribosomal protein L6 [1] (data from MRSA252) SAUSA300_RS12090 50S ribosomal protein L5 [1] (data from MRSA252) SAUSA300_RS12105 30S ribosomal protein S17 [1] (data from MRSA252) SAUSA300_RS12115 50S ribosomal protein L16 [1] (data from MRSA252) SAUSA300_RS12125 50S ribosomal protein L22 [1] (data from MRSA252) SAUSA300_RS12130 30S ribosomal protein S19 [1] (data from MRSA252) SAUSA300_RS12135 50S ribosomal protein L2 [1] (data from MRSA252) SAUSA300_RS12140 50S ribosomal protein L23 [1] (data from MRSA252) SAUSA300_RS12145 50S ribosomal protein L4 [1] (data from MRSA252) SAUSA300_RS12150 50S ribosomal protein L3 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)