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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS12135 [old locus tag: SAUSA300_2201 ]
- pan locus tag?: SAUPAN005699000
- symbol: SAUSA300_RS12135
- pan gene symbol?: rplB
- synonym:
- product: 50S ribosomal protein L2
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS12135 [old locus tag: SAUSA300_2201 ]
- symbol: SAUSA300_RS12135
- product: 50S ribosomal protein L2
- replicon: chromosome
- strand: -
- coordinates: 2368564..2369397
- length: 834
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2368564..2369397) NCBI
- BioCyc: SAUSA300_RS12135 BioCyc
- MicrobesOnline: see SAUSA300_2201
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGGCTATTAAAAAGTATAAGCCAATAACAAATGGTCGTCGTAATATGACTTCGTTAGAT
TTCGCAGAAATCACGAAAACTACACCTGAAAAGTCATTATTAAAACCGCTACCGAAAAAA
GCGGGACGTAACAACCAAGGTAAATTGACTGTAAGACACCATGGTGGTGGACACAAACGT
CAATACCGTGTTATCGATTTCAAACGTAACAAAGATGGTATCAATGCAAAAGTTGATTCT
ATTCAATATGATCCAAACCGCTCAGCAAACATCGCTTTAGTTGTATATGCAGACGGTGAA
AAACGATATATCATTGCTCCTAAAGGATTAGAAGTAGGTCAAATCGTTGAAAGTGGTGCT
GAAGCTGACATCAAAGTTGGTAACGCATTACCATTACAAAACATTCCAGTTGGTACAGTA
GTACACAACATCGAGCTTAAACCTGGTAAAGGTGGACAAATCGCTCGTTCAGCTGGTGCA
AGTGCTCAAGTACTTGGTAAAGAAGGTAAATACGTATTAATCAGATTAAGATCTGGTGAA
GTTCGTATGATCTTATCTACTTGCCGTGCTACAATCGGTCAAGTTGGTAACCTACAACAC
GAATTAGTTAACGTTGGTAAAGCCGGACGTTCAAGATGGAAAGGTATCCGTCCAACAGTT
CGTGGTTCTGTAATGAACCCTAACGATCACCCACACGGTGGTGGTGAAGGTCGTGCTCCT
ATCGGTAGACCATCTCCAATGTCACCATGGGGTAAACCTACGCTTGGTAAGAAAACTCGT
CGTGGTAAAAAATCATCAGACAAACTTATCGTTCGTGGACGTAAGAAAAAATAA60
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834
⊟Protein[edit | edit source]
Protein Data Bank: 4WCE
Protein Data Bank: 4WF9
Protein Data Bank: 4WFA
Protein Data Bank: 4WFB
Protein Data Bank: 5HKV
Protein Data Bank: 5HL7
Protein Data Bank: 5LI0
Protein Data Bank: 5ND8
Protein Data Bank: 5ND9
Protein Data Bank: 5NRG
Protein Data Bank: 5TCU
⊟General[edit | edit source]
- locus tag: SAUSA300_RS12135 [old locus tag: SAUSA300_2201 ]
- symbol: SAUSA300_RS12135
- description: 50S ribosomal protein L2
- length: 277
- theoretical pI: 11.4477
- theoretical MW: 30154.8
- GRAVY: -0.664621
⊟Function[edit | edit source]
- TIGRFAM: Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uL2 (TIGR01171; HMM-score: 460.3)
- TheSEED: see SAUSA300_2201
- PFAM: KOW (CL0107) Ribosomal_L2_C; Ribosomal Proteins L2, C-terminal domain (PF03947; HMM-score: 192.1)and 1 moreOB (CL0021) Ribosomal_L2; Ribosomal Proteins L2, RNA binding domain (PF00181; HMM-score: 122.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.01024
- TAT(Tat/SPI): 0.002969
- LIPO(Sec/SPII): 0.002384
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446908216 NCBI
- RefSeq: WP_000985472 NCBI
- UniProt: see SAUSA300_2201
⊟Protein sequence[edit | edit source]
- MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRNNQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKRYIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGASAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKKK
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS01635 5'-nucleotidase, lipoprotein e(P4) family [1] (data from MRSA252) SAUSA300_RS01940 30S ribosomal protein S6 [1] (data from MRSA252) SAUSA300_RS02050 hypothetical protein [1] (data from MRSA252) SAUSA300_RS02800 50S ribosomal protein L1 [1] (data from MRSA252) SAUSA300_RS02840 30S ribosomal protein S7 [1] (data from MRSA252) SAUSA300_RS04885 NAD(+) kinase [1] (data from MRSA252) SAUSA300_RS05325 ribonuclease J 1 [1] (data from MRSA252) SAUSA300_RS06320 ribonuclease J 2 [1] (data from MRSA252) SAUSA300_RS06430 glycerol-3-phosphate responsive antiterminator [1] (data from MRSA252) SAUSA300_RS07430 DNA-binding protein HU [1] (data from MRSA252) SAUSA300_RS08665 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [1] (data from MRSA252) SAUSA300_RS08730 50S ribosomal protein L21 [1] (data from MRSA252) SAUSA300_RS09015 universal stress protein [1] (data from MRSA252) SAUSA300_RS09090 30S ribosomal protein S4 [1] (data from MRSA252) SAUSA300_RS09205 membrane protein [1] (data from MRSA252) SAUSA300_RS11985 30S ribosomal protein S9 [1] (data from MRSA252) SAUSA300_RS12015 50S ribosomal protein L17 [1] (data from MRSA252) SAUSA300_RS12025 30S ribosomal protein S11 [1] (data from MRSA252) SAUSA300_RS12030 30S ribosomal protein S13 [1] (data from MRSA252) SAUSA300_RS12055 50S ribosomal protein L15 [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS12075 50S ribosomal protein L6 [1] (data from MRSA252) SAUSA300_RS12105 30S ribosomal protein S17 [1] (data from MRSA252) SAUSA300_RS12110 50S ribosomal protein L29 [1] (data from MRSA252) SAUSA300_RS12115 50S ribosomal protein L16 [1] (data from MRSA252) SAUSA300_RS12120 30S ribosomal protein S3 [1] (data from MRSA252) SAUSA300_RS12125 50S ribosomal protein L22 [1] (data from MRSA252) SAUSA300_RS12140 50S ribosomal protein L23 [1] (data from MRSA252) SAUSA300_RS12145 50S ribosomal protein L4 [1] (data from MRSA252) SAUSA300_RS12215 lipid II:glycine glycyltransferase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)