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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS08680 [old locus tag: SAUSA300_1593 ]
  • pan locus tag?: SAUPAN004243000
  • symbol: SAUSA300_RS08680
  • pan gene symbol?: secDF
  • synonym:
  • product: protein translocase subunit SecDF

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS08680 [old locus tag: SAUSA300_1593 ]
  • symbol: SAUSA300_RS08680
  • product: protein translocase subunit SecDF
  • replicon: chromosome
  • strand: -
  • coordinates: 1746579..1748858
  • length: 2280
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    GTGAAGAAAAGTAGTAGAATAATTGCGTTCTTGCTTTTAGTCGTATTGTTGTTTGCCGGA
    ATGGCTGCAACTTATAAAAGTGTTATAAAGAATGTCAATTTAGGACTTGATTTGCAAGGT
    GGATTTGAGGTGCTTTATCAAGTCGATCCTTTAAATAAAGGCGACAAAATAGATAAAAAA
    GCCTTACAATCAACAGCGCAAACATTAGAAAATCGTGTTAACGTTTTAGGTGTTTCGGAA
    CCTAAAATTCAAGTTGAAGAACCTAATAGAATTAGAGTACAACTTGCTGGTGTAACAGAC
    CAGAATGAAGCACGTAAAATTTTATCTTCTCAAGCTAATTTAACAATTCGCGATGCAGAA
    GATAAAGTAAAATTAAGCGGTTCTGATATTAAACAGGGATCTGCCAAACAAGAATTTAAA
    CAGGAAACAAACCAGCCTACAGTTACTTTTAAAGTAAAAGACAAAAACAAATTTAAAAAA
    GTAACGGAAGAAATCTCTAAGAAACGTGATAATGTCATGGTTGTTTGGTTAGATTTCAAA
    AAAGGTGACAGCTATAAAAAAGAAGCACAAAAGAAAAACCCTAAATTTATTTCTGCAGCA
    AGTGTTGATCAACCAATCAATTCTGATAGCGTAGAAATCTCGGGTGGATTTAAAGGACAA
    GAAGGCGTTAAAAAAGCAAAACAAATCGCCGAATTGTTAAATGCAGGTTCTTTACCAGTT
    GACTTGAAAGAAATTTATTCAAACTCAGTAGGTGCCCAATTTGGTCAAGATGCATTAGAT
    AAAACTGTGTTTGCATCGTTTATTGGTGTAGCATTAATTTATTTATTCATGCTTGGATTC
    TACCGATTACCTGGTTTAGTAGCGATTATTGCATTGACAACTTATATCTATCTAACGTTA
    GTAGCATTTAATTTCATTTCCGGGGTTCTAACTTTACCAGGATTAGCGGCGTTGGTATTA
    GGTGTAGGTATGGCTGTAGATGCCAATATTATCATGTATGAGCGTATTAAGGATGAACTT
    CGAATAGGTAGAACGATAAAGCAAGCCTTTTCTAAAGCAAACAAAAGTTCATTCCTAACA
    ATTTTTGATTCTAACTTAACAACAGTTATCGCCGCAGCAGTATTATTCTTCTTCGGTGAA
    AGTTCAGTTAAAGGTTTCGCGACAATGTTATTATTAGGTATTCTAATGATCTTTGTTACA
    GCCGTGTTCTTATCAAGATTCTTATTATCATTACTTGTTTCATCAAATATATTCAAAAAT
    CAATTTTGGTTATTTGGTGTTAAAAAGAATAAACGACATGATATTAATGAAGGTGTAGAT
    GTTCATGACCTTAAAACTTCATTCGAGAAATGGAATTTTGTTAAATTAGCTAAGCCATTA
    ATTGGAGTAAGTATTTTAATTGTAGTTGTCGGTTTAGTTATTCTTTATATCTTCAAGTTA
    AACTTAGGAATTGATTTCTCAAGTGGTACACGCGTAGATTTCCAATCTAAACAAGCAATT
    ACTCAACAAAAGGTAGAGCAAGTTGTAAAAGATAGTGGACTTAAAGCGGATCAAATTCAG
    ATCAATGGTAAAGACAATAAAGTAGCAACTGTTCAATTTAAAGACGATTTAACGCGTGCT
    CAAGATAATAAATTGAGTGATAATATTAAATCGAAATTCGGTGACACACCTCAAATTAAC
    ACTGTATCACCAATTATTGGACAGGAATTAGCAAAAAATGCAATGCTTGCATTAATCTAT
    GCATCGATAGGTATCATCATCTATGTATCATTACGATTTGAATGGCGCATGGGTCTTTCA
    TCTGTATTGGCATTATTACATGATGTATTCATTATAGTAGCGATTTTCAGTTTATTTAGA
    ATTGAAGTAGATTTAACATTTATCGCCGCTGTATTAACAATTGTCGGTTATTCAATTAAT
    GATACAATCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACG
    ACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCA
    ATTAATACAGTATTAACAGTTATTGTAGTAGTAGTTGCTATACTATTCTTCGGTGCTCCT
    ACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCA
    ATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAATCGCCG
    AAACACAAATTAGTTGTATATAAAGAAAAGAAATCGAACGATGAAAAGATTTTAGTTTAA
    60
    120
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    300
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    420
    480
    540
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    720
    780
    840
    900
    960
    1020
    1080
    1140
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    1260
    1320
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    1560
    1620
    1680
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    1980
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    2160
    2220
    2280

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS08680 [old locus tag: SAUSA300_1593 ]
  • symbol: SAUSA300_RS08680
  • description: protein translocase subunit SecDF
  • length: 759
  • theoretical pI: 10.3191
  • theoretical MW: 84253.3
  • GRAVY: 0.234124

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein SecD (TIGR01129; HMM-score: 386.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein, SecD/SecF family (TIGR00916; HMM-score: 338.2)
    and 3 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein SecF (TIGR00966; HMM-score: 276.2)
    Metabolism Transport and binding proteins Unknown substrate efflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 58.2)
    Metabolism Transport and binding proteins Unknown substrate transport protein (TIGR00833; HMM-score: 27.2)
  • TheSEED: see SAUSA300_1593
  • PFAM:
    RND_permease (CL0322) SecD_SecF; Protein export membrane protein (PF02355; HMM-score: 256)
    and 6 more
    no clan defined Sec_GG; SecD/SecF GG Motif (PF07549; HMM-score: 39.6)
    RND_permease (CL0322) MMPL; MMPL family (PF03176; HMM-score: 39.2)
    no clan defined Selenoprotein_S; Selenoprotein S (SelS) (PF06936; HMM-score: 12.7)
    POTRA (CL0191) POTRA; Surface antigen variable number repeat (PF07244; HMM-score: 12.4)
    no clan defined SecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 11.4)
    BRCT-like (CL0459) RTT107_BRCT_6; Regulator of Ty1 transposition protein 107 BRCT domain (PF16771; HMM-score: 8.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 22 aa
    • SP(Sec/SPI): 0.427618
    • TAT(Tat/SPI): 0.00166
    • LIPO(Sec/SPII): 0.373685
    • Cleavage Site: CS pos: 22-23. GMA-AT. Pr: 0.1458
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEVLYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQNEARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKKVTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFKGQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLFMLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYERIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLLGILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEKWNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQVVKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPIIGQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVDLTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSINTVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSPKHKLVVYKEKKSNDEKILV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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