PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1337 [new locus tag: SAUSA300_RS07290 ]
• pan locus tag^?: SAUPAN003904000
• symbol: SAUSA300_1337
• pan gene symbol^?: gpsB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1337 [new locus tag: SAUSA300_RS07290 ]
• symbol: SAUSA300_1337
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1502503..1502847
• length: 345
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913581 NCBI
• RefSeq: YP_494034 NCBI
• BioCyc: see SAUSA300_RS07290
• MicrobesOnline: 1292852 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  
  ATGTCAGATGTTTCATTGAAATTATCAGCAAAAGATATTTATGAAAAAGATTTTGAAAAA
  ACGATGGCTCGTGGCTATAGAAGAGAAGAAGTAGATGCATTTTTAGATGACATTATTGCT
  GATTATCAAAAAATGGCCGATATGAATAATGAAGTTGTAAAATTATCAGAAGAGAATCAT
  AAACTTAAAAAAGAATTAGAAGAATTAAGACTACGTGTAGCAACATCAAGACCTCAGGAC
  AATAAAAGTTTTTCTTCGAATAATACAACAACAAATACATCTTCAAATAATGTAGATATT
  TTAAAACGTATTTCAAACTTAGAAAAAGCTGTATTTGGTAAATAA
  60
  120
  180
  240
  300
  345
  

⊟Protein

⊟General

• locus tag: SAUSA300_1337 [new locus tag: SAUSA300_RS07290 ]
• symbol: SAUSA300_1337
• description: hypothetical protein
• length: 114
• theoretical pI: 5.97468
• theoretical MW: 13150.7
• GRAVY: -0.867544

⊟Function

• TIGRFAM:
  DivIVA domain (TIGR03544; HMM-score: 52.8)
  and 4 more
  Cellular processes Cellular processes Sporulation and germination transcription factor, RsfA family (TIGR02894; HMM-score: 16.8)
  Signal transduction Regulatory functions DNA interactions transcription factor, RsfA family (TIGR02894; HMM-score: 16.8)
  DivIVA domain repeat protein (TIGR03543; HMM-score: 16.1)
  SH3 domain protein (TIGR04211; HMM-score: 13.5)
• TheSEED  :
  • Cell division protein GpsB, coordinates the switch between cylindrical and septal cell wall synthesis by re-localization of PBP1
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Control of cell elongation - division cycle in Bacilli  Cell division protein GpsB, coordinates the switch between cylindrical and septal cell wall synthesis by re-localization of PBP1
• PFAM:
  no clan defined DivIVA; DivIVA protein (PF05103; HMM-score: 45.9)
  and 19 more
  YabB; Initiation control protein YabA (PF06156; HMM-score: 24.9)
  HMMR_C; Hyaluronan mediated motility receptor C-terminal (PF15908; HMM-score: 19.7)
  Ax_dynein_light; Axonemal dynein light chain (PF10211; HMM-score: 18.9)
  bZIP (CL0018) bZIP_1; bZIP transcription factor (PF00170; HMM-score: 16.7)
  no clan defined HOOK; HOOK protein (PF05622; HMM-score: 16.3)
  CtIP_N; Tumour-suppressor protein CtIP N-terminal domain (PF10482; HMM-score: 15.8)
  FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 15.7)
  no clan defined ABC_tran_CTD; ABC transporter C-terminal domain (PF16326; HMM-score: 15)
  DASH_Spc19; Spc19 (PF08287; HMM-score: 14.9)
  HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 14.7)
  SlyX; SlyX (PF04102; HMM-score: 14.5)
  CENP-H; Centromere protein H (CENP-H) (PF05837; HMM-score: 14.2)
  Ribo_L29 (CL0346) Ribosomal_L29; Ribosomal L29 protein (PF00831; HMM-score: 13.8)
  no clan defined TolA_bind_tri; TolA binding protein trimerisation (PF16331; HMM-score: 13.7)
  bZIP (CL0018) bZIP_2; Basic region leucine zipper (PF07716; HMM-score: 13.3)
  no clan defined Cnn_1N; Centrosomin N-terminal motif 1 (PF07989; HMM-score: 12.5)
  IncA; IncA protein (PF04156; HMM-score: 12.3)
  Herpes_BLRF2; Herpesvirus BLRF2 protein (PF05812; HMM-score: 12)
  CC2-LZ; Leucine zipper of domain CC2 of NEMO, NF-kappa-B essential modulator (PF16516; HMM-score: 10.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001477
  • TAT(Tat/SPI): 0.000385
  • LIPO(Sec/SPII): 0.000377
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162117 NCBI
• RefSeq: YP_494034 NCBI
• UniProt: Q2FGZ4 UniProt

⊟Protein sequence

• MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQKMADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILKRISNLEKAVFGK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1337 < SAUSA300_1338 < SAUSA300_1339

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications