PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1873 [new locus tag: SAUSA300_RS10245 ]
• pan locus tag^?: SAUPAN004914000
• symbol: SAUSA300_1873
• pan gene symbol^?: murT
• synonym:
• product: Mur ligase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1873 [new locus tag: SAUSA300_RS10245 ]
• symbol: SAUSA300_1873
• product: Mur ligase family protein
• replicon: chromosome
• strand: -
• coordinates: 2032757..2034070
• length: 1314
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913850 NCBI
• RefSeq: YP_494525 NCBI
• BioCyc: see SAUSA300_RS10245
• MicrobesOnline: 1293388 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAGACAGTGGACGGCAATCCATCTAGCGAAATTGGCGCGTAAAGCAAGTAGAGCAGTA
  GGTAAAAGAGGAACAGATTTACCTGGACAAATCGCTAGAAAAGTGGATACAGATATATTA
  AGAAAATTAGCAGAGCAAGTTGATGATATTGTATTTATCAGTGGAACAAATGGTAAAACA
  ACGACTTCAAACTTAATTGGACATACTTTAAAAGCAAATAATATTCAAATTATACACAAT
  AATGAAGGTGCTAATATGGCTGCAGGTATAACTTCTGCATTCATCATGCAATCAACACCT
  AAGACTAAAATTGCGGTAATCGAAATTGATGAAGGTTCGATTCCACGTGTGTTAAAAGAA
  GTTACACCTTCAATGATGGTATTTACTAATTTCTTTAGAGATCAAATGGATCGCTTCGGT
  GAAATTGATATTATGGTTAATAACATTGCAGAGACAATTAGTAATAAAGGCATCAAATTA
  TTGCTAAATGCTGATGATCCATTTGTGAGTCGTTTGAAAATCGCAAGTGATACGATTGTG
  TACTATGGTATGAAAGCACATGCCCATGAATTTGAACAAAGTACGATGAATGAAAGTAGA
  TATTGTCCAAACTGTGGTCGCTTATTGCAATACGATTATATTCATTATAATCAAATTGGT
  CATTATCACTGTCAGTGTGGTTTCAAACGAGAGCAAGCAAAATATGAAATATCAAGTTTT
  GATGTGGCACCGTTTTTATATTTAAATATCAATGATGAAAAATATGATATGAAAATTGCA
  GGTGACTTTAACGCTTATAACGCGTTAGCAGCATATACTGTTTTAAGAGAGCTAGGGTTA
  AATGAACAAACAATTAAAAATGGCTTTGAAACGTATACATCAGACAATGGTCGTATGCAG
  TACTTTAAAAAAGAACGAAAAGAAGCGATGATCAATTTAGCTAAAAATCCTGCAGGAATG
  AATGCAAGTTTATCAGTTGGTGAACAATTAGAAGGCGAAAAAGTGTATGTTATTTCGCTA
  AATGATAACGCTGCAGATGGTCGAGATACTTCATGGATTTATGATGCAGATTTTGAAAAA
  TTATCTAAGCAACAAATTGAAGCTATCATCGTGACAGGTACACGAGCAGAAGAACTTCAA
  TTGCGATTGAAGTTAGCAGAGGTTGAAGTACCAATTATAGTTGAGCGTGATATTTATAAA
  GCAACGGCAAAGACTATGGATTATAAAGGTTTCACAGTTGCAATACCAAACTATACATCA
  TTAGCGCCTATGCTTGAACAATTAAACCGTTCGTTTGAAGGAGGTCAATCATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1873 [new locus tag: SAUSA300_RS10245 ]
• symbol: SAUSA300_1873
• description: Mur ligase family protein
• length: 437
• theoretical pI: 6.52509
• theoretical MW: 49205.7
• GRAVY: -0.349428

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 64.6)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 61.2)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 58.3)
  and 6 more
  Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 46.7)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 45.8)
  Cell structure Cell envelope Other poly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 23.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 22)
  HprK-related kinase A (TIGR04352; HMM-score: 16.9)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 14.1)
• TheSEED  :
  • proposed amino acid ligase found clustered with an amidotransferase
• PFAM:
  no clan defined DUF1727; Domain of unknown function (DUF1727) (PF08353; HMM-score: 101.6)
  and 6 more
  Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 68.6)
  Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 16.6)
  RING (CL0229) zf-C3H2C3; Zinc-finger (PF17122; HMM-score: 13.5)
  Zn_Beta_Ribbon (CL0167) OrfB_Zn_ribbon; Putative transposase DNA-binding domain (PF07282; HMM-score: 12.8)
  TF_Zn_Ribbon; TFIIB zinc-binding (PF08271; HMM-score: 12.7)
  zf-RING_7; C4-type zinc ribbon domain (PF02591; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.025235
  • TAT(Tat/SPI): 0.001676
  • LIPO(Sec/SPII): 0.003894
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159963 NCBI
• RefSeq: YP_494525 NCBI
• UniProt: A0A0H2XFN5 UniProt

⊟Protein sequence

• MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLAEQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKTKIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKLLLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQIGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLRELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEKVYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPIIVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1872 < SAUSA300_1873

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Daniela Münch, Terry Roemer, Sang Ho Lee, Marianne Engeser, Hans Georg Sahl, Tanja Schneider
Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus.
PLoS Pathog: 2012, 8(1);e1002509
[PubMed:22291598] [WorldCat.org] [DOI] (I p)