PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1872 [new locus tag: SAUSA300_RS10240 ]
• pan locus tag^?: SAUPAN004913000
• symbol: SAUSA300_1872
• pan gene symbol^?: gatD
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1872 [new locus tag: SAUSA300_RS10240 ]
• symbol: SAUSA300_1872
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2032024..2032755
• length: 732
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914474 NCBI
• RefSeq: YP_494524 NCBI
• BioCyc: see SAUSA300_RS10240
• MicrobesOnline: 1293387 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGCATGAATTGACTATTTATCATTTTATGTCAGATAAATTGAATTTATACAGTGATATA
  GGAAATATTATTGCTTTAAGACAACGTGCTAAAAAACGAAATATTAAAGTTAATGTCGTA
  GAAATCAATGAAACAGAAGGTATTACCTTTGATGAATGTGATATTTTCTTTATCGGTGGT
  GGAAGTGATAGAGAACAAGCATTAGCAACAAAAGAATTAAGTAAAATTAAGACACCACTT
  AAAGAAGCGATTGAAGATGGTATGCCGGGATTAACGATTTGTGGAGGCTATCAATTTTTA
  GGGAAAAAATATATCACGCCTGATGGTACAGAATTAGAAGGGTTAGGTATTTTAGATTTT
  TATACTGAATCAAAGACAAACCGATTAACAGGAGATATTGTTATCGAAAGTGATACTTTT
  GGAACTATTGTAGGTTTTGAAAATCACGGTGGTAGAACATATCATGATTTCGGTACACTT
  GGTCATGTTACTTTTGGTTATGGTAATAATGATGAAGATAAAAAAGAAGGCATTCATTAT
  AAAAATTTATTAGGTACTTATTTACATGGACCAATTTTACCTAAAAATTACGAAATCACT
  GATTATCTGTTAGAAAAAGCTTGTGAACGTAAGGGTATTCCGTTTGAGCCTAAAGAAATA
  GATAATGAAGCGGAAATACAAGCGAAACAAGTATTAATAGACAGAGCAAATAGACAGAAG
  AAATCTCGTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1872 [new locus tag: SAUSA300_RS10240 ]
• symbol: SAUSA300_1872
• description: hypothetical protein
• length: 243
• theoretical pI: 5.41856
• theoretical MW: 27429.8
• GRAVY: -0.530864

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 55)
  and 4 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, glutaminase subunit Pdx2 (TIGR03800; EC 2.6.-.-; HMM-score: 19.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyrinic acid a,c-diamide synthase (TIGR00379; EC 6.3.1.-; HMM-score: 19)
  Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 16.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 14.4)
• TheSEED  :
  • Lipid II isoglutaminyl synthase (glutamine-hydrolyzing), GatD subunit (EC 6.3.5.13)
• PFAM:
  Glutaminase_I (CL0014) GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 148.6)
  and 3 more
  GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 23.4)
  DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 14.3)
  no clan defined DUF4154; Domain of unknown function (DUF4154) (PF13689; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003013
  • TAT(Tat/SPI): 0.000175
  • LIPO(Sec/SPII): 0.000358
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161962 NCBI
• RefSeq: YP_494524 NCBI
• UniProt: A0A0H2XJQ7 UniProt

⊟Protein sequence

• MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINETEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGKKYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTLGHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPKEIDNEAEIQAKQVLIDRANRQKKSR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1872 < SAUSA300_1873

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Daniela Münch, Terry Roemer, Sang Ho Lee, Marianne Engeser, Hans Georg Sahl, Tanja Schneider
Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus.
PLoS Pathog: 2012, 8(1);e1002509
[PubMed:22291598] [WorldCat.org] [DOI] (I p)