From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001921
  • pan locus tag?: SAUPAN004913000
  • symbol: JSNZ_001921
  • pan gene symbol?: gatD
  • synonym:
  • product: type 1 glutamine amidotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001921
  • symbol: JSNZ_001921
  • product: type 1 glutamine amidotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1934804..1935535
  • length: 732
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCATGAATTGACTATTTATCATTTTATGTCAGATAAATTGAATTTATACAGTGATATA
    GGAAATATTATTGCTTTAAGACAACGTGCTAAAAAACGAAATATTAAAGTTAATGTCGTA
    GAAATCAATGAAACAGAAGGTATTACCTTTGATGAATGTGATATTTTCTTTATCGGTGGT
    GGAAGTGATAGAGAACAAGCATTAGCAACAAAAGAATTAAGTAAAATTAAGACACCACTT
    AAAGAAGCGATTGAAGATGGTATGCCGGGATTAACAATTTGTGGAGGCTATCAATTTTTA
    GGAAAAAAATATATCACGCCTGATGGTACAGAATTAGAAGGGTTAGGTATTTTAGATTTT
    TATACTGAATCAAAGACAAACCGATTAACAGGAGATATTATTATCGAAAGTGATACTTTT
    GGAACTATTGTAGGTTTTGAAAATCACGGTGGTAGAACATATCATGATTTCGGTACACTT
    GGTCATGTCACTTTTGGTTATGGTAATAATGATGAAGATAAAAAAGAAGGCATTCATTAT
    AAAAATTTATTAGGTACTTATTTACACGGACCAATTTTACCTAAAAATTACGAAATCACT
    GATTATCTGTTAGAAAAAGCTTGTGAACGTAAGGGTATTCCGTTTGAGCCTAAAGAAATA
    GATAATGAAGCGGAAATACAAGCGAAACAAGTATTAATAGACAGAGCAAATAGACAGAAG
    AAATCTCGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    732

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001921
  • symbol: JSNZ_001921
  • description: type 1 glutamine amidotransferase
  • length: 243
  • theoretical pI: 5.41856
  • theoretical MW: 27443.9
  • GRAVY: -0.52963

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 54.9)
    and 4 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, glutaminase subunit Pdx2 (TIGR03800; EC 2.6.-.-; HMM-score: 19.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyrinic acid a,c-diamide synthase (TIGR00379; EC 6.3.1.-; HMM-score: 19)
    Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 16.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 14.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Glutaminase_I (CL0014) GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 147.7)
    and 3 more
    GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 23.4)
    DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 14.9)
    no clan defined DUF4154; YfiR/HmsC-like (PF13689; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9222
    • Cytoplasmic Membrane Score: 0.0108
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0669
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003013
    • TAT(Tat/SPI): 0.000175
    • LIPO(Sec/SPII): 0.000358
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINETEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGKKYITPDGTELEGLGILDFYTESKTNRLTGDIIIESDTFGTIVGFENHGGRTYHDFGTLGHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPKEIDNEAEIQAKQVLIDRANRQKKSR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Daniela Münch, Terry Roemer, Sang Ho Lee, Marianne Engeser, Hans Georg Sahl, Tanja Schneider
Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus.
PLoS Pathog: 2012, 8(1);e1002509
[PubMed:22291598] [WorldCat.org] [DOI] (I p)

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_001921&oldid=107562"