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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001922
  • pan locus tag?: SAUPAN004914000
  • symbol: murT
  • pan gene symbol?: murT
  • synonym:
  • product: lipid II isoglutaminyl synthase subunit MurT

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001922
  • symbol: murT
  • product: lipid II isoglutaminyl synthase subunit MurT
  • replicon: chromosome
  • strand: -
  • coordinates: 1935537..1936850
  • length: 1314
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGACAGTGGACGGCAATCCATCTAGCGAAATTGGCGCGTAAAGCAAGTAGAGCAGTA
    GGTAAAAGAGGAACAGATTTACCTGGACAAATCGCTAGAAAAGTGGATACAGATATATTA
    AGAAAATTAGCAGAGCAAGTTGATGATATTGTATTTATCAGTGGAACAAATGGTAAAACA
    ACGACTTCAAACTTAATTGGACATACTTTAAAAGCAAATAATATTCAAATTATACACAAT
    AATGAAGGTGCTAATATGGCTGCAGGTATAACTTCTGCATTCATCATGCAATCAACACCT
    AAGACTAAAATTGCGGTAATCGAAATTGATGAAGGTTCGATTCCACGTGTGTTAAAAGAA
    GTTACACCTTCAATGATGGTATTTACTAATTTCTTTAGAGATCAAATGGATCGCTTCGGT
    GAAATTGATATTATGGTTAATAACATTGCAGAGACAATTAGTAATAAAGGCATCAAATTA
    TTGCTAAATGCTGATGATCCATTTGTGAGTCGTTTGAAAATCGCAAGTGATACGATTGTG
    TACTATGGTATGAAAGCACATGCCCATGAATTTGAACAAAGTACGATGAATGAAAGTAGA
    TATTGTCCAAACTGTGGTCGCTTATTGCAATACGATTATATTCATTATAATCAAATTGGT
    CATTATCACTGTCAGTGTGGTTTCAAACGAGAGCAAGCAAAATATGAAATATCAAGTTTT
    GATGTGGCACCGTTTTTACATTTAAATATCAATGATGAAAAATATGATATGAAAATTGCA
    GGTGACTTTAACGCTTATAACGCGTTAGCAGCATATACTGTTTTAAGAGAGCTAGGGTTA
    AATGAACAAGCAATTAAAAATGGCTTTGAAACGTATACATCAGACAATGGTCGTATGCAG
    TACTTTAAAAAAGAACGAAAAGAAGCGATGATCAATTTAGCTAAAAATCCTGCAGGAATG
    AATGCAAGTTTATCAGTTGGTGAACAATTAGAAGGCGAAAAAGTGTATGTTATTTCGCTA
    AATGATAACGCTGCAGATGGTCGAGATACTTCATGGATTTATGATGCAGATTTTGAAAAA
    TTATCTAAGCAACAAATTGAAGCTATCATCGTGACAGGTACACGAGCAGAAGAACTTCAA
    TTGCGATTGAAGTTAGCAGAGGTTGAAGTAGCAATTATAGTTGAGCGTGATATTTATAAA
    GCAACGGCAAAGACTATGGATTATAAAGGTTTCACAGTTGCAATACCAAACTATACATCA
    TTAGCGCCTATGCTTGAACAATTAAACCGTTCGTTTGAAGGAGGTCAATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1314

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001922
  • symbol: MurT
  • description: lipid II isoglutaminyl synthase subunit MurT
  • length: 437
  • theoretical pI: 6.61113
  • theoretical MW: 49123.6
  • GRAVY: -0.340275

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 65.4)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 61.5)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 58.5)
    and 6 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 46.1)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 45.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 22.6)
    Cell structure Cell envelope Other poly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 20.8)
    HprK-related kinase A (TIGR04352; HMM-score: 16.9)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 14.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Mur_ligase_C_sf (CL0794) MurT_C; MurT ligase C-terminal (PF08353; HMM-score: 104.1)
    and 5 more
    P-loop_NTPase (CL0023) Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 64.7)
    Mur_ligase_C_sf (CL0794) Mur_ligase_C; Mur ligase, glutamate ligase domain (PF02875; HMM-score: 17.7)
    Zn_Beta_Ribbon (CL0167) Zn_Ribbon_TF; TFIIB zinc-binding (PF08271; HMM-score: 14.4)
    RING (CL0229) zf-C3H2C3; Zinc-finger (PF17122; HMM-score: 13.4)
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_9; C4-type zinc ribbon domain (PF02591; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.6968
    • Cytoplasmic Membrane Score: 0.2935
    • Cell wall & surface Score: 0.0025
    • Extracellular Score: 0.0073
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025235
    • TAT(Tat/SPI): 0.001676
    • LIPO(Sec/SPII): 0.003894
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLAEQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKTKIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKLLLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQIGHYHCQCGFKREQAKYEISSFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYTVLRELGLNEQAIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEKVYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVAIIVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Daniela Münch, Terry Roemer, Sang Ho Lee, Marianne Engeser, Hans Georg Sahl, Tanja Schneider
Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus.
PLoS Pathog: 2012, 8(1);e1002509
[PubMed:22291598] [WorldCat.org] [DOI] (I p)

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