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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004914000
- symbol?: murT
- synonym:
- description?: UDP-N-acetylmuramate--alanine ligase
- UDP-N-acetylmuramate--alanine ligase
- Mur ligase family protein
- UDP-N-acetylmuramyl tripeptide synthetase
- lipid II isoglutaminyl synthase subunit MurT
- Mur ligase
- UDP-N-acetylmuramyl tripeptide synthetase, putative
- UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase
- murein peptide ligase
- mur ligase middle domain protein
- putative amino acid ligase
- UDP-N-acetylmuramyl tripeptide synthase
- UDP-N-acetylmuramyl tripeptide synthetase-like
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 5103082..5104416
- synteny block?: BlockID0038200
- occurrence?: in 100% of 34 strains
murT : lipid II isoglutaminyl synthase ligase subunit [1]
• A crystal structure is available : 6GS2
During cell wall synthesis, the staphylococcal peptidoglycan precursor Lipid II is sequestered at the inner leaflet of the cell membrane for final modifications prior to translocation to the extracellular environment. Two final modifications must occur prior to translocation: addition of a pentaglycine crosslink to the peptide stem lysine and an optional amidation of the peptide stem γ-ᴅ-glutamate to generate isoglutamine. The GatD-MurT complex catalyzes the conversion of peptide stem γ-ᴅ-glutamate to isoglutamine. GatD liberates ammonia from L-glutamine and channels it to the MurT subunit which activates the α-carboxylate of peptide stem γ-ᴅ-glutamate with ATP and exchanges the acid-phosphate (or acid-adenylate) with ammonia to generate isoglutamine. Most γ-glutamyl residues are modified and this modification enhances staphylococcal viability and methicillin resistance.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1848
08BA02176:
C248_1965
11819-97:
MS7_1927
6850:
RSAU_001779
71193:
ST398NM01_1985
ECT-R 2:
ECTR2_1763
ED133:
SAOV_1991
ED98:
SAAV_1957
HO 5096 0412:
SAEMRSA15_17990
JH1:
SaurJH1_1980
JH9:
SaurJH9_1946
JKD6008:
SAA6008_01912 (murE2)
JKD6159:
SAA6159_01823 (murE2)
JSNZ:
JSNZ_001922 (murT)
LGA251:
SARLGA251_17740
M013:
M013TW_1928
MRSA252:
SAR1983
MSHR1132:
SAMSHR1132_17360
MSSA476:
SAS1814
Mu3:
SAHV_1877
Mu50:
SAV1892
MW2:
MW1833
RF122:
SAB1824c
ST398:
SAPIG1985
T0131:
SAT0131_02017
TCH60:
HMPREF0772_11251 (murE)
TW20:
SATW20_18860
USA300_TCH1516:
USA300HOU_1890 (murE2)
VC40:
SAVC_08720
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLAEQVDDIVFISGTNGKT
N315 MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDVLRKLAEQVDDIVFISGTNGKT
NCTC8325 MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLAEQVDDIVFISGTNGKT
Newman MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLAEQVDDIVFISGTNGKT
USA300_FPR3757 MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLAEQVDDIVFISGTNGKT
**************************************:*********************
COL TTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKTKIAVIEIDEGSIPRVLKE
N315 TTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKTKIAVIEIDEGSIPRVLKE
NCTC8325 TTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKTKIAVIEIDEGSIPRVLKE
Newman TTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKTKIAVIEIDEGSIPRVLKE
USA300_FPR3757 TTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKTKIAVIEIDEGSIPRVLKE
************************************************************
COL VTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKLLLNADDPFVSRLKIASDTIV
N315 VTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKLLLNADDPFVSRLKIASDTIV
NCTC8325 VTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKLLLNADDPFVSRLKIASDTIV
Newman VTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKLLLNADDPFVSRLKIASDTIV
USA300_FPR3757 VTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKLLLNADDPFVSRLKIASDTIV
************************************************************
COL YYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQIGHYHCQCGFKREQAKYEISSF
N315 YYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQIGHYHCQCGFKREQAKYEISSF
NCTC8325 YYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQIGHYHCQCGFKREQAKYEISSF
Newman YYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQIGHYHCQCGFKREQAKYEISSF
USA300_FPR3757 YYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQIGHYHCQCGFKREQAKYEISSF
************************************************************
COL DVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLRELGLNEQTIKNGFETYTSDNGRMQ
N315 DVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLRELGLNEQTIKNGFETYTSDNGRMQ
NCTC8325 DVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLRELGLNEQTIKNGFETYTSDNGRMQ
Newman DVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLRELGLNEQTIKNGFETYTSDNGRMQ
USA300_FPR3757 DVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLRELGLNEQTIKNGFETYTSDNGRMQ
************************************************************
COL YFKKERKEAMINLAKNPAGMNASLSVGEQLEGEKVYVISLNDNAADGRDTSWIYDADFEK
N315 YFKKERKEAMINLAKNPAGMNASLSVGEQLEGEKVYVISLNDNAADGRDTSWIYDADFEK
NCTC8325 YFKKERKEAMINLAKNPAGMNASLSVGEQLEGEKVYVISLNDNAADGRDTSWIYDADFEK
Newman YFKKERKEAMINLAKNPAGMNASLSVGEQLEGEKVYVISLNDNAADGRDTSWIYDADFEK
USA300_FPR3757 YFKKERKEAMINLAKNPAGMNASLSVGEQLEGEKVYVISLNDNAADGRDTSWIYDADFEK
************************************************************
COL LSKQQIEAIIVTGTRAEELQLRLKLAEVEVPIIVERDIYKATAKTMDYKGFTVAIPNYTS
N315 LSKQQIEAIIVTGTRAEELQLRLKLAEVEVPIIVERDIYKATAKTMDYKGFTVAIPNYTS
NCTC8325 LSKQQIEAIIVTGTRAEELQLRLKLAEVEVPIIVERDIYKATAKTMDYKGFTVAIPNYTS
Newman LSKQQIEAIIVTGTRAEELQLRLKLAEVEVPIIVERDIYKATAKTMDYKGFTVAIPNYTS
USA300_FPR3757 LSKQQIEAIIVTGTRAEELQLRLKLAEVEVPIIVERDIYKATAKTMDYKGFTVAIPNYTS
************************************************************
COL LAPMLEQLNRSFEGGQS
N315 LAPMLEQLNRSFEGGQS
NCTC8325 LAPMLEQLNRSFEGGQS
Newman LAPMLEQLNRSFEGGQS
USA300_FPR3757 LAPMLEQLNRSFEGGQS
*****************
- ↑ Daniela Münch, Terry Roemer, Sang Ho Lee, Marianne Engeser, Hans Georg Sahl, Tanja Schneider
Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus.
PLoS Pathog: 2012, 8(1);e1002509
[PubMed:22291598] [WorldCat.org] [DOI] (I p)Erik R Nöldeke, Lena M Muckenfuss, Volker Niemann, Anna Müller, Elena Störk, Georg Zocher, Tanja Schneider, Thilo Stehle
Structural basis of cell wall peptidoglycan amidation by the GatD/MurT complex of Staphylococcus aureus.
Sci Rep: 2018, 8(1);12953
[PubMed:30154570] [WorldCat.org] [DOI] (I e)Maria D Barbuti, Ine S Myrbråten, Danae Morales Angeles, Morten Kjos
The cell cycle of Staphylococcus aureus: An updated review.
Microbiologyopen: 2023, 12(1);e1338
[PubMed:36825883] [WorldCat.org] [DOI] (I p)