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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004913000
- symbol?: gatD
- synonym:
- description?: glutamine amidotransferase
- glutamine amidotransferase
- type 1 glutamine amidotransferase
- CobB/CobQ-like glutamine amidotransferase
- cobyric acid synthase
- cobyric acid synthase, putative
- adenosylcobyric acid synthase (glutamine-hydrolyzing)
- CobB/CobQ domain-containing protein glutamine amidotransferase
- CobB/CobQ-like glutamine amidotransferase domain-containing protein
- cobB/CobQ-like glutamine amidotransferase domain protein
- cobyric acid synthase CobQ
- putative amidotransferase, putative cobyricacid synthase
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 5102349..5103080
- synteny block?: BlockID0038190
- occurrence?: in 100% of 34 strains
gatD : lipid II isoglutaminyl synthase glutamine amidotransferase subunit [1]
• A crystal structure is available : 6GS2
During cell wall synthesis, the staphylococcal peptidoglycan precursor Lipid II is sequestered at the inner leaflet of the cell membrane for final modifications prior to translocation to the extracellular environment. Two final modifications must occur prior to translocation: addition of a pentaglycine crosslink to the peptide stem lysine and an optional amidation of the peptide stem γ-ᴅ-glutamate to generate isoglutamine. The GatD-MurT complex catalyzes the conversion of peptide stem γ-ᴅ-glutamate to isoglutamine. GatD liberates ammonia from L-glutamine and channels it to the MurT subunit which amidates the glutamate α-carboxylate to generate isoglutamine. Most residues are modified and this modification enhances staphylococcal viability and methicillin resistance.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1847 (cobQ)
08BA02176:
C248_1964
11819-97:
MS7_1926
6850:
RSAU_001778 (cobQ)
71193:
ST398NM01_1984
ECT-R 2:
ECTR2_1762
ED133:
SAOV_1990
ED98:
SAAV_1956
HO 5096 0412:
SAEMRSA15_17980
JH1:
SaurJH1_1979
JH9:
SaurJH9_1945
JKD6008:
SAA6008_01911
JKD6159:
SAA6159_01822
JSNZ:
JSNZ_001921
LGA251:
SARLGA251_17730
M013:
M013TW_1927
MRSA252:
SAR1982
MSHR1132:
SAMSHR1132_17350
MSSA476:
SAS1813
Mu3:
SAHV_1876
Mu50:
SAV1891
MW2:
MW1832
RF122:
SAB1823c
ST398:
SAPIG1984
T0131:
SAT0131_02016 (clpP)
TCH60:
HMPREF0772_11252
TW20:
SATW20_18850
USA300_TCH1516:
USA300HOU_1889
VC40:
SAVC_08715
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINETEGITFDECDIFFIGG
N315 MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINETEGITFDECDIFFIGG
NCTC8325 MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINETEGITFDECDIFFIGG
Newman MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINETEGITFDECDIFFIGG
USA300_FPR3757 MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINETEGITFDECDIFFIGG
************************************************************
COL GSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGKKYITPDGTELEGLGILDF
N315 GSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGKKYITPDGTELEGLGILDF
NCTC8325 GSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGKKYITPDGTELEGLGILDF
Newman GSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGKKYITPDGTELEGLGILDF
USA300_FPR3757 GSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGKKYITPDGTELEGLGILDF
************************************************************
COL YTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTLGHVTFGYGNNDEDKKEGIHY
N315 YTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTLGHVTFGYGNNDEDKKEGIHY
NCTC8325 YTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTLGHVTFGYGNNDEDKKEGIHY
Newman YTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTLGHVTFGYGNNDEDKKEGIHY
USA300_FPR3757 YTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTLGHVTFGYGNNDEDKKEGIHY
************************************************************
COL KNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPKEIDNEAEIQAKQVLIDRANRQK
N315 KNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPKEIDNEAEIQAKQVLIDRANRQK
NCTC8325 KNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPKEIDNEAEIQAKQVLIDRANRQK
Newman KNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPKEIDNEAEIQAKQVLIDRANRQK
USA300_FPR3757 KNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPKEIDNEAEIQAKQVLIDRANRQK
************************************************************
COL KSR
N315 KSR
NCTC8325 KSR
Newman KSR
USA300_FPR3757 KSR
***
- ↑ Daniela Münch, Terry Roemer, Sang Ho Lee, Marianne Engeser, Hans Georg Sahl, Tanja Schneider
Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus.
PLoS Pathog: 2012, 8(1);e1002509
[PubMed:22291598] [WorldCat.org] [DOI] (I p)Erik R Nöldeke, Lena M Muckenfuss, Volker Niemann, Anna Müller, Elena Störk, Georg Zocher, Tanja Schneider, Thilo Stehle
Structural basis of cell wall peptidoglycan amidation by the GatD/MurT complex of Staphylococcus aureus.
Sci Rep: 2018, 8(1);12953
[PubMed:30154570] [WorldCat.org] [DOI] (I e)Maria D Barbuti, Ine S Myrbråten, Danae Morales Angeles, Morten Kjos
The cell cycle of Staphylococcus aureus: An updated review.
Microbiologyopen: 2023, 12(1);e1338
[PubMed:36825883] [WorldCat.org] [DOI] (I p)