From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1311 [new locus tag: SAUSA300_RS07140 ]
  • pan locus tag?: SAUPAN003843000
  • symbol: murG
  • pan gene symbol?: murG
  • synonym:
  • product: undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1311 [new locus tag: SAUSA300_RS07140 ]
  • symbol: murG
  • product: undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1444430..1445500
  • length: 1071
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACGAAAATCGCATTTACCGGAGGGGGAACAGTTGGACACGTATCAGTAAATTTAAGT
    TTAATTCCAACTGCATTATCACAAGGTTATGAAGCGCTTTATATTGGTTCTAAAAATGGT
    ATTGAAAGAGAAATGATTGAATCACAACTACCAGAAATTAAGTATTATCCTATTTCGAGT
    GGTAAATTAAGAAGATATATTTCTTTAGAAAATGCCAAAGACGTATTTAAAGTATTGAAA
    GGTATTCTTGATGCTCGTAAAGTTTTGAAAAAAGAAAAACCTGATCTATTATTTTCAAAA
    GGTGGATTTGTATCTGTGCCTGTTGTTATTGCAGCCAAATCATTAAATATACCAACTCTT
    ATTCATGAATCTGACTTAACACCAGGATTAGCGAATAAGATAGCACTTAAATTTGCCAAG
    AAAATATATACAACATTTGAAGAAACGCTAAACTACTTACCTAAAGAGAAAGCTGATTTT
    ATTGGAGCAACAATTCGAGAAGATTTAAAAAATGGTAATGCACATAATGGTTATCAATTA
    ACAGGCTTTAATGAAAATAAAAAAGTTTTACTCGTTATGGGTGGAAGCTTAGGAAGTAAA
    AAATTAAATAGCATTATTCGCGAAAACTTAGATGCATTATTACAACAATATCAAGTGATA
    CATTTAACTGGTAAAGGATTAAAAGATGCTCAAGTTAAAAAATCAGGATATATACAATAT
    GAATTTGTTAAAGAGGATTTAACAGATTTATTAGCAATTACGGATACAGTAATAAGTAGA
    GCTGGATCAAATGCGATTTATGAGTTCTTAACATTACGTATACCAATGTTATTAGTACCA
    TTAGGTTTAGATCAATCCCGAGGCGACCAAATTGACAATGCAAATCATTTTGCTGATAAA
    GGATATGCTAAAGCGATTGATGAAGAACAATTAACAGCACAAATTTTATTACAAGAACTA
    AATGAAATGGAACAGGAAAGAACTCGAATTATCAATAATATGAAATCGTATGAACAAAGT
    TATACGAAAGAAGCTTTATTTGATAAGATGATTAAAGACGCATTGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1071

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1311 [new locus tag: SAUSA300_RS07140 ]
  • symbol: MurG
  • description: undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase
  • length: 356
  • theoretical pI: 9.13983
  • theoretical MW: 39696.5
  • GRAVY: -0.229494

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.1.227?  ExPASy
    Undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (TIGR01133; EC 2.4.1.227; HMM-score: 244)
    and 2 more
    Cellular processes Cellular processes Toxin production and resistance glycosyltransferase, MGT family (TIGR01426; EC 2.4.1.-; HMM-score: 40.6)
    Hypothetical proteins Conserved conserved hypothetical protein (TIGR00661; HMM-score: 29.7)
  • TheSEED  :
    • UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)
  • PFAM:
    GT-B (CL0113) Glyco_transf_28; Glycosyltransferase family 28 N-terminal domain (PF03033; HMM-score: 128.5)
    and 6 more
    Glyco_tran_28_C; Glycosyltransferase family 28 C-terminal domain (PF04101; HMM-score: 95)
    UDPGT; UDP-glucoronosyl and UDP-glucosyl transferase (PF00201; HMM-score: 29.7)
    Glyco_trans_1_3; Glycosyl transferase family 1 (PF13528; HMM-score: 17.9)
    no clan defined Oberon_cc; Coiled-coil region of Oberon (PF16312; HMM-score: 14.2)
    GT-B (CL0113) Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 13.6)
    no clan defined TFIIA_gamma_N; Transcription initiation factor IIA, gamma subunit, helical domain (PF02268; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.310983
    • TAT(Tat/SPI): 0.006498
    • LIPO(Sec/SPII): 0.013024
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIEREMIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGGFVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADFIGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQVIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPMLLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNMKSYEQSYTKEALFDKMIKDALN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_2570(arcA)arginine deiminase  [1] (data from MRSA252)
    SAUSA300_0532(fusA)elongation factor G  [1] (data from MRSA252)
    SAUSA300_1633(gap)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    SAUSA300_2055(murA)UDP-N-acetylglucosamine 1-carboxyvinyltransferase  [1] (data from MRSA252)
    SAUSA300_0524(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SAUSA300_0533(tuf)elongation factor Tu  [1] (data from MRSA252)
    SAUSA300_2066(upp)uracil phosphoribosyltransferase  [1] (data from MRSA252)
    SAUSA300_0235L-lactate dehydrogenase  [1] (data from MRSA252)
    SAUSA300_0637dihydroxyacetone kinase subunit DhaL  [1] (data from MRSA252)
    SAUSA300_0658LysR family transcriptional regulator  [1] (data from MRSA252)
    SAUSA300_0844hypothetical protein  [1] (data from MRSA252)
    SAUSA300_0912enoyl-(acyl carrier protein) reductase  [1] (data from MRSA252)
    SAUSA300_1656universal stress protein  [1] (data from MRSA252)
    SAUSA300_2484hydroxymethylglutaryl-CoA synthase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_1311&oldid=94711"