PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2055 [new locus tag: SAUSA300_RS11315 ]
• pan locus tag^?: SAUPAN005388000
• symbol: murA
• pan gene symbol^?: murA1
• synonym:
• product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2055 [new locus tag: SAUSA300_RS11315 ]
• symbol: murA
• product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
• replicon: chromosome
• strand: -
• coordinates: 2218346..2219611
• length: 1266
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912790 NCBI
• RefSeq: YP_494701 NCBI
• BioCyc: see SAUSA300_RS11315
• MicrobesOnline: 1293570 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
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  421
  481
  541
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  901
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  1021
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  1261
  
  ATGGATAAAATAGTAATCAAAGGTGGAAATAAATTAACGGGTGAAGTTAAAGTAGAAGGT
  GCTAAAAATGCAGTATTACCAATATTGACAGCATCTTTATTAGCTTCTGATAAACCGAGC
  AAATTAGTTAATGTTCCAGCTTTAAGTGATGTAGAAACAATAAATAATGTATTAACAACT
  TTAAATGCTGACGTTACATACAAAAAGGACGAAAATGCTGTTGTCGTTGATGCAACAAAG
  ACTCTAAATGAAGAGGCACCATATGAATATGTTAGTAAAATGCGTGCAAGTATTTTAGTT
  ATGGGACCTCTTTTAGCAAGACTAGGACATGCTATTGTTGCATTGCCTGGTGGTTGTGCA
  ATTGGAAGTAGACCGATTGAGCAACACATTAAAGGTTTTGAAGCTTTAGGCGCAGAAATT
  CATCTTGAAAATGGTAATATTTATGCTAATGCTAAAGATGGATTAAAAGGTACATCAATT
  CATTTAGATTTTCCAAGTGTAGGAGCAACACAAAATATTATTATGGCAGCATCATTAGCT
  AAGGGTAAGACTTTAATTGAAAATGCAGCTAAAGAACCTGAAATTGTCGATTTAGCAAAC
  TACATTAATGAAATGGGTGGTAGAATTACTGGTGCTGGTACAGACACAATTACAATCAAT
  GGTGTAGAATCATTACATGGTGTAGAACATGCTATCATTCCAGATAGAATTGAAGCAGGC
  ACATTACTAATCGCTGGTGCTATAACGCGTGGTGATATTTTTGTACGTGATGCAATCAAA
  GAACATATGGCGAGTTTAGTCTATAAACTAGAAGAAATGGGCGTTGAATTGGACTATCAA
  GAAGATGGTATTCGTGTACGTGCTGAAGGGGAATTACAACCTGTAGACATCAAAACTCTA
  CCACATCCTGGATTCCCGACTGATATGCAATCACAAATGATGGCATTGTTATTAACGGCA
  AATGGTCATAAAGTCGTAACCGAAACTGTTTTTGAAAACCGTTTTATGCATGTTGCAGAG
  TTCAAACGTATGAATGCTAATATCAATGTAGAAGGTCGTAGTGCTAAACTTGAAGGTAAA
  AGTCAATTGCAAGGTGCACAAGTTAAAGCGACTGATTTAAGAGCAGCAGCCGCCTTAATT
  TTAGCTGGATTAGTTGCTGATGGTAAAACAAGCGTTACTGAATTAACGCACCTAGATAGA
  GGCTATGTTGACTTACACGGTAAATTGAAGCAATTAGGTGCAGACATTGAACGTATTAAC
  GATTAA
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  1266
  

⊟Protein

⊟General

• locus tag: SAUSA300_2055 [new locus tag: SAUSA300_RS11315 ]
• symbol: MurA
• description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
• length: 421
• theoretical pI: 5.61613
• theoretical MW: 45053.5
• GRAVY: -0.0351544

⊟Function

• reaction:
  EC 2.5.1.7?  ExPASy
  UDP-N-acetylglucosamine 1-carboxyvinyltransferase Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine 1-carboxyvinyltransferase (TIGR01072; EC 2.5.1.7; HMM-score: 580.2)
  and 1 more
  Metabolism Amino acid biosynthesis Aromatic amino acid family 3-phosphoshikimate 1-carboxyvinyltransferase (TIGR01356; EC 2.5.1.19; HMM-score: 71.2)
• TheSEED  :
  • UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7)
  and 1 more
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7)
• PFAM:
  EPT_RTPC (CL0290) EPSP_synthase; EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (PF00275; HMM-score: 354.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.133088
  • TAT(Tat/SPI): 0.001887
  • LIPO(Sec/SPII): 0.031419
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161215 NCBI
• RefSeq: YP_494701 NCBI
• UniProt: A0A0H2XGP3 UniProt

⊟Protein sequence

• MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVNVPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMGPLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSIHLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTITINGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVELDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENRFMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSVTELTHLDRGYVDLHGKLKQLGADIERIND

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: fabZ < murA < SAUSA300_2056

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications