⊟Summary[edit | edit source]

pan ID^?: SAUPAN006375000
symbol^?: —
synonym:
description^?: N-acetylmuramoyl-L-alanine amidase
- N-acetylmuramoyl-L-alanine amidase
- amidase domain-containing protein
- exported protein
- mannosyl-glycoendo-beta-N-acetylglucosaminidase family protein
- mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase family protein
- N-acetylmuramidase
- N-acetylmuramoyl-L-alanine amidase, family 4
- N-acetylmuramoyl-L-alanine amidase domain-containing protein
- N-acetylmuramoyl-L-alanine amidase family protein
strand^?: +
coordinates^?: 6415000..6416883
synteny block^?: BlockID0050840
occurrence^?: in 100% of 34 strains

scaH : peptidoglycan N-acetylglucosaminidase Aly ^[1]

• A crystal structure is available: 5T1Q

Staphylocci produce multiple cell wall remodeling enzymes that differ in where they digest peptidoglycan, their localization on the membrane and regulation of their activity
- Staphylococci produce one bifunctional N-acetylglucosaminidase/amidase: Atl (AtlA)
- Staphylococci produce three N-acetylglucosaminidases: SagA (LytD, AtlE), SagB and ScaH (Aly)
- Staphylococci produce two bifunctional amidase/endopeptidases: LytN and EssH
- Staphylococci produce two lytic transglycosylases: SceD and IsaA
- Staphylococci produce two monofunctional amidases: SsaA and Sle1
- Staphylococci produce three monofunctional endopeptidases: LytH, LytM and LytU
- Staphylococci produce five uncharacterized probable monofunctional amidases: (SsaA2), (SsaA3), (SsaA4), (SsaA5) and (SsaA6)

⊟Orthologs[edit | edit source]

COL:

N315:

NCTC8325:

Newman:

USA300_FPR3757:

04-02981:

SA2981_2583

08BA02176:

C248_2712

11819-97:

MS7_2649

6850:

RSAU_002487

71193:

ST398NM01_2694

ECT-R 2:

ECTR2_2498

ED133:

SAOV_2693

ED98:

SAAV_2714

HO 5096 0412:

SAEMRSA15_25490

JH1:

SaurJH1_2724

JH9:

SaurJH9_2668

JKD6008:

SAA6008_02703

JKD6159:

SAA6159_02540

JSNZ:

JSNZ_002634

LGA251:

SARLGA251_24160

M013:

M013TW_2625

MRSA252:

SAR2723

MSHR1132:

SAMSHR1132_24680

MSSA476:

SAS2530

Mu3:

SAHV_2628

Mu50:

SAV2644

MW2:

MW2565

RF122:

SAB2519

ST398:

SAPIG2694

T0131:

SAT0131_02912

TCH60:

HMPREF0772_10543

TW20:

SATW20_27820

USA300_TCH1516:

USA300HOU_2644

VC40:

SAVC_12100

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]

alignment of orthologues:
CLUSTAL format alignment by MAFFT L-INS-i (v7.307)

COL             MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSKKSNDDETSKDTTSKDI
N315            MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSKKSNDDETSKDTTSKDT
NCTC8325        MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSKKSNDDETSKDTTSKDI
Newman          MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSKKSNDDETSKDTTSKDI
USA300_FPR3757  MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSKKSNDDETSKDTTSKDI
                ***********************************************************

COL             DKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQTNINQLLTKNKYDDNYSLT
N315            DKADNNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQTNINQLLTKNKYDDNYSLT
NCTC8325        DKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQTNINQLLTKNKYDDNYSLT
Newman          DKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQTNINQLLTKNKYDDNYSLT
USA300_FPR3757  DKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQTNINQLLTKNKYDDNYSLT
                ****:*******************************************************

COL             TLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKNDTDTQSSKQDKADNQKAPKSN
N315            TLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKNDTDTQSSKQDKADNQKAPKSN
NCTC8325        TLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKNDTDTQSSKQDKADNQKAPKSN
Newman          TLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKNDTDTQSSKQDKADNQKAPKSN
USA300_FPR3757  TLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKNDTDTQSSKQDKADNQKAPKSN
                ************************************************************

COL             NTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSSKDNQSMSDSALDSILDQYSEDA
N315            NTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSSKDNQSMSDSALDSILDQYSEDA
NCTC8325        NTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSSKDNQSMSDSALDSILDQYSEDA
Newman          NTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSSKDNQSMSDSALDSILDQYSEDA
USA300_FPR3757  NTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSSKDNQSMSDSALDSILDQYSEDA
                ************************************************************

COL             KKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKSKPAQSFNNDVNQKDTRATSLFETD
N315            KKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKSKPAQSFNNDVNQKDTRATSLFETD
NCTC8325        KKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKSKPAQSFNNDVNQKDTRATSLFETD
Newman          KKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKSKPAQSFNNDVNQKDTRATSLFETD
USA300_FPR3757  KKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKSKPAQSFNNDVNQKDTRATSLFETD
                **:*********************************************************

COL             PSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRIGQDNDIYASVMIAQAILESDSGRSAL
N315            PSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRIGQDNDIYASVMIAQAILESDSGRSAL
NCTC8325        PSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRIGQDNDIYASVMIAQAILESDSGRSAL
Newman          PSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRIGQDNDIYASVMIAQAILESDSGRSAL
USA300_FPR3757  PSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRIGQDNDIYASVMIAQAILESDSGRSAL
                ************************************************************

COL             AKSPNHNLFGIKGAFEGNSVPFNTLEADGNQLYSINAGFRKYPSTKESLKDYSDLIKNGI
N315            AKSPNHNLFGIKGAFEGNSVPFNTLEADGNKLYSINAGFRKYPSTKESLKDYSDLIKNGI
NCTC8325        AKSPNHNLFGIKGAFEGNSVPFNTLEADGNQLYSINAGFRKYPSTKESLKDYSDLIKNGI
Newman          AKSPNHNLFGIKGAFEGNSVPFNTLEADGNQLYSINAGFRKYPSTKESLKDYSDLIKNGI
USA300_FPR3757  AKSPNHNLFGIKGAFEGNSVPFNTLEADGNQLYSINAGFRKYPSTKESLKDYSDLIKNGI
                ******************************:*****************************

COL             DGNRTIYKPTWKSEADSYKDATSHLSKTYATDPNYAKKLNSIIKHYQLTQFDDERMPDLD
N315            DGNRTIYKPTWKSEADSYKDATSHLSKTYATDPNYAKKLNSIIKHYQLTQFDDERMPDLD
NCTC8325        DGNRTIYKPTWKSEADSYKDATSHLSKTYATDPNYAKKLNSIIKHYQLTQFDDERMPDLD
Newman          DGNRTIYKPTWKSEADSYKDATSHLSKTYATDPNYAKKLNSIIKHYQLTQFDDERMPDLD
USA300_FPR3757  DGNRTIYKPTWKSEADSYKDATSHLSKTYATDPNYAKKLNSIIKHYQLTQFDDERMPDLD
                ************************************************************

COL             KYERSIKDYDDSSDEFKPFREVSDSMPYPHGQCTWYVYNRMKQFGTSISGDLGDAHNWNN
N315            KYERSIKDYDDSSDEFKPFREVSDSMPYPHGQCTWYVYNRMKQFGTSISGDLGDAHNWNN
NCTC8325        KYERSIKDYDDSSDEFKPFREVSDSMPYPHGQCTWYVYNRMKQFGTSISGDLGDAHNWNN
Newman          KYERSIKDYDDSSDEFKPFREVSDSMPYPHGQCTWYVYNRMKQFGTSISGDLGDAHNWNN
USA300_FPR3757  KYERSIKDYDDSSDEFKPFREVSDSMPYPHGQCTWYVYNRMKQFGTSISGDLGDAHNWNN
                ************************************************************

COL             RAQYRDYQVSHTPKRHAAVVFEAGQFGADQHYGHVAFVEKVNSDGSIVISESNVKGLGII
N315            RAQYRDYQVSHTPKRHAAVVFEAGQFGADQHYGHVAFVEKVNSDGSIVISESNVKGLGII
NCTC8325        RAQYRDYQVSHTPKRHAAVVFEAGQFGADQHYGHVAFVEKVNSDGSIVISESNVKGLGII
Newman          RAQYRDYQVSHTPKRHAAVVFEAGQFGADQHYGHVAFVEKVNSDGSIVISESNVKGLGII
USA300_FPR3757  RAQYRDYQVSHTPKRHAAVVFEAGQFGADQHYGHVAFVEKVNSDGSIVISESNVKGLGII
                ************************************************************

COL             SHRTINAAAAEELSYITGK
N315            SHRTINAAAAEELSYITGK
NCTC8325        SHRTINAAAAEELSYITGK
Newman          SHRTINAAAAEELSYITGK
USA300_FPR3757  SHRTINAAAAEELSYITGK
                *******************

↑ Min Wang, Girbe Buist, Jan Maarten van Dijl
Staphylococcus aureus cell wall maintenance - the multifaceted roles of peptidoglycan hydrolases in bacterial growth, fitness, and virulence.
FEMS Microbiol Rev: 2022, 46(5);
[PubMed:35675307] [WorldCat.org] [DOI] (I p)Min Wang, Xiaofang Li, Francis M Cavallo, Harita Yedavally, Sjouke Piersma, Elisa J M Raineri, Elias Vera Murguia, Jeroen Kuipers, Zhenhua Zhang, Jan Maarten van Dijl, Girbe Buist
Functional profiling of CHAP domain-containing peptidoglycan hydrolases of Staphylococcus aureus USA300 uncovers potential targets for anti-staphylococcal therapies.
Int J Med Microbiol: 2024, 316;151632
[PubMed:39142057] [WorldCat.org] [DOI] (I p)Richard Wheeler, Robert D Turner, Richard G Bailey, Bartłomiej Salamaga, Stéphane Mesnage, Sharifah A S Mohamad, Emma J Hayhurst, Malcolm Horsburgh, Jamie K Hobbs, Simon J Foster
Bacterial Cell Enlargement Requires Control of Cell Wall Stiffness Mediated by Peptidoglycan Hydrolases.
mBio: 2015, 6(4);e00660
[PubMed:26220963] [WorldCat.org] [DOI] (I e)

[1] Min Wang, Girbe Buist, Jan Maarten van Dijl
Staphylococcus aureus cell wall maintenance - the multifaceted roles of peptidoglycan hydrolases in bacterial growth, fitness, and virulence.
FEMS Microbiol Rev: 2022, 46(5);
[PubMed:35675307] [WorldCat.org] [DOI] (I p)Min Wang, Xiaofang Li, Francis M Cavallo, Harita Yedavally, Sjouke Piersma, Elisa J M Raineri, Elias Vera Murguia, Jeroen Kuipers, Zhenhua Zhang, Jan Maarten van Dijl, Girbe Buist
Functional profiling of CHAP domain-containing peptidoglycan hydrolases of Staphylococcus aureus USA300 uncovers potential targets for anti-staphylococcal therapies.
Int J Med Microbiol: 2024, 316;151632
[PubMed:39142057] [WorldCat.org] [DOI] (I p)Richard Wheeler, Robert D Turner, Richard G Bailey, Bartłomiej Salamaga, Stéphane Mesnage, Sharifah A S Mohamad, Emma J Hayhurst, Malcolm Horsburgh, Jamie K Hobbs, Simon J Foster
Bacterial Cell Enlargement Requires Control of Cell Wall Stiffness Mediated by Peptidoglycan Hydrolases.
mBio: 2015, 6(4);e00660
[PubMed:26220963] [WorldCat.org] [DOI] (I e)

[1]

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Contents

⊟Summary[edit | edit source]

⊟Orthologs[edit | edit source]

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757

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⊟Summary[edit | edit source]

⊟Orthologs[edit | edit source]

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]