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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS02585 [old locus tag: SA0448 ]
- pan locus tag?: SAUPAN002227000
- symbol: SA_RS02585
- pan gene symbol?: metS
- synonym:
- product: methionine--tRNA ligase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1921ATGGCTAAAGAAACATTTTATATAACAACCCCAATATACTATCCTAGTGGGAATTTACAT
ATAGGACATGCATATTCTACAGTGGCTGGAGATGTTATTGCAAGATATAAGAGAATGCAA
GGATATGATGTTCGTTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAA
GCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAA
CAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGAACAACTGAAGAACGT
CATAAACATGTCGTTGAGCAAGTGTTTGAACGTTTATTAAAGCAAGGTGATATCTATTTA
GGTGAATATGAAGGTTGGTATTCTGTTCCGGATGAAACATACTATACAGAGTCACAATTA
GTAGACCCACAATACGAAAACGGTAAAATTATTGGTGGCAAAAGTCCAGATTCTGGACAC
GAAGTTGAACTAGTTAAAGAAGAAAGTTATTTCTTTAATATTAGTAAATATACAGACCGT
TTATTAGAGTTCTATGACCAAAATCCAGATTTTATACAACCACCATCAAGAAAAAATGAA
ATGATTAACAACTTCATTAAACCAGGACTTGCTGATTTAGCTGTTTCTCGTACATCATTT
AACTGGGGTGTCCATGTTCCGTCTAATCCAAAACATGTTGTTTATGTTTGGATTGATGCG
TTAGTTAACTATATTTCAGCATTAGGCTATTTATCAGATGATGAGTCACTATTTAACAAA
TACTGGCCAGCAGATATTCATTTAATGGCTAAGGAAATTGTGCGATTCCACTCAATTATT
TGGCCTATTTTATTGATGGCATTAGACTTACCGTTACCTAAAAAAGTCTTTGCACATGGT
TGGATTTTGATGAAAGATGGAAAAATGAGTAAATCTAAAGGTAATGTTGTAGACCCTAAT
ATTTTAATTGATCGCTATGGTTTAGATGCTACACGTTATTATCTAATGCGTGAATTACCA
TTTGGTTCAGATGGCGTATTTACACCTGAAGCATTTGTTGAGCGTACAAATTTCGATCTA
GCAAATGACTTAGGTAACTTAGTAAACCGTACGATTTCTATGGTTAATAAGTACTTTGAT
GGCGAATTACCAGCGTATCAAGGTCCACTTCATGAATTAGATGAAGAAATGGAAGCTATG
GCTTTAGAAACAGTGAAAAGCTACACTGAAAGCATGGAAAGTTTGCAATTTTCTGTGGCA
TTATCTACGGTATGGAAGTTTATAAGTAGAACGAATAAGTATATTGACGAAACAACGCCT
TGGGTATTAGCTAAGGACGATAGCCAAAAAGATATGTTAGGCAATGTAATGGCTCACTTA
GTTGAAAATATTCGTTATGCAGCTGTATTATTACGTCCATTCTTAACACATGCGCCGAAA
GAGATTTTTGAACAATTGAACATAAACAATCCTCAATTTATGGAATTTAGTAGTTTAGAG
CAATATGGTGTGCTTACTGAGTCAATTATGGTTACTGGGCAACCTAAACCTATTTTCCCA
AGATTGGATAGCGAAGCGGAAATTGCATATATCAAAGAATCAATGCAACCGCCTGCTACT
GAAGAGGAAAAAGAAGAGATTCCTAGCAAACCTCAAATTGATATTAAAGACTTTGATAAA
GTTGAAATTAAGGCAGCAACGATTATTGATGCTGAACATGTTAAGAAGTCAGATAAGCTT
TTAAAAATTCAAGTAGACTTAGATTCTGAACAAAGACAAATTGTATCAGGAATTGCCAAA
TTCTATACACCAGATGATATTATTGGTAAAAAAGTAGCAGTTGTTACTAACCTGAAACCA
GCTAAATTAATGGGACAAAAATCTGAAGGTATGATATTATCTGCTGAAAAAGATGGTGTA
TTAACCTTAGTAAGTTTACCAAGTGCAATTCCAAATGGTGCAGTGATTAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA_RS02585 [old locus tag: SA0448 ]
- symbol: SA_RS02585
- description: methionine--tRNA ligase
- length: 657
- theoretical pI: 4.86419
- theoretical MW: 74872.9
- GRAVY: -0.33379
⊟Function[edit | edit source]
- reaction: EC 6.1.1.10? ExPASyMethionine--tRNA ligase ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
- TIGRFAM: Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 564.5)and 12 moreProtein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 148.9)Protein synthesis tRNA aminoacylation methionine--tRNA ligase, beta subunit (TIGR00399; EC 6.1.1.10; HMM-score: 139.1)Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 114.4)Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 112)Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 81.2)Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 62)Protein fate Protein and peptide secretion and trafficking export-related chaperone protein CsaA (TIGR02222; HMM-score: 38.7)Protein fate Protein folding and stabilization export-related chaperone protein CsaA (TIGR02222; HMM-score: 38.7)Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, beta subunit (TIGR00472; EC 6.1.1.20; HMM-score: 27.5)Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 26.2)Protein synthesis tRNA aminoacylation arginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 24)Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 16.4)
- TheSEED: see SA0448
- PFAM: HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 387.2)and 10 moretRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 105.5)OB (CL0021) tRNA_bind; Putative tRNA binding domain (PF01588; HMM-score: 89.1)HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 56.5)DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA (PF08264; HMM-score: 35.3)HUP (CL0039) tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 25.3)Pilus (CL0327) PilM; PilM (PF07419; HMM-score: 14.6)no clan defined ARL2_Bind_BART; The ARF-like 2 binding protein BART (PF11527; HMM-score: 13)HUP (CL0039) tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 12.6)no clan defined T4_neck-protein; Virus neck protein (PF11649; HMM-score: 10.8)HUP (CL0039) tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 9.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.014505
- TAT(Tat/SPI): 0.001142
- LIPO(Sec/SPII): 0.001179
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDVRYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHKHVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGHEVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRTSFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRFHSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYYLMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHELDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQKDMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTESIMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAATIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLMGQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SA_RS11130 (deoA) pyrimidine-nucleoside phosphorylase [2] (data from MRSA252) SA_RS09855 (gatA) glutamyl-tRNA(Gln) amidotransferase subunit A [2] (data from MRSA252) SA_RS00185 serine--tRNA ligase [2] (data from MRSA252) SA_RS00215 50S ribosomal protein L9 [2] (data from MRSA252) SA_RS00310 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia [2] (data from MRSA252) SA_RS01275 formate acetyltransferase [2] (data from MRSA252) SA_RS01365 L-lactate dehydrogenase [2] (data from MRSA252) SA_RS01960 acetyl-CoA acyltransferase [2] (data from MRSA252) SA_RS02015 30S ribosomal protein S6 [2] (data from MRSA252) SA_RS02025 30S ribosomal protein S18 [2] (data from MRSA252) SA_RS02095 alkyl hydroperoxide reductase subunit C [2] (data from MRSA252) SA_RS02145 IMP dehydrogenase [2] (data from MRSA252) SA_RS02405 methionine ABC transporter substrate-binding protein [2] (data from MRSA252) SA_RS02490 YbaB/EbfC family nucleoid-associated protein [2] (data from MRSA252) SA_RS02640 ribose-phosphate pyrophosphokinase [2] (data from MRSA252) SA_RS02650 50S ribosomal protein L25/general stress protein Ctc [2] (data from MRSA252) SA_RS02710 cysteine synthase [2] (data from MRSA252) SA_RS02810 pyridoxal 5'-phosphate synthase lyase subunit PdxS [2] (data from MRSA252) SA_RS02905 50S ribosomal protein L11 [2] (data from MRSA252) SA_RS02910 50S ribosomal protein L1 [2] (data from MRSA252) SA_RS02915 50S ribosomal protein L10 [2] (data from MRSA252) SA_RS02935 DNA-directed RNA polymerase subunit beta' [2] (data from MRSA252) SA_RS02950 30S ribosomal protein S7 [2] (data from MRSA252) SA_RS02955 elongation factor G [2] (data from MRSA252) SA_RS02960 elongation factor Tu [2] (data from MRSA252) SA_RS03155 phosphate acetyltransferase [2] (data from MRSA252) SA_RS03380 metal ABC transporter substrate-binding protein [2] (data from MRSA252) SA_RS04140 aldehyde dehydrogenase [2] (data from MRSA252) SA_RS04145 phosphoglycerate kinase [2] (data from MRSA252) SA_RS04150 triose-phosphate isomerase [2] (data from MRSA252) SA_RS04155 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [2] (data from MRSA252) SA_RS04160 enolase [2] (data from MRSA252) SA_RS04575 NADH dehydrogenase [2] (data from MRSA252) SA_RS04660 NAD-specific glutamate dehydrogenase [2] (data from MRSA252) SA_RS04680 glucose-6-phosphate isomerase [2] (data from MRSA252) SA_RS04710 hypothetical protein [2] (data from MRSA252) SA_RS04865 oligoendopeptidase F [2] (data from MRSA252) SA_RS05350 pyruvate dehydrogenase E1 component subunit alpha [2] (data from MRSA252) SA_RS05365 dihydrolipoyl dehydrogenase [2] (data from MRSA252) SA_RS05620 thiol reductase thioredoxin [2] (data from MRSA252) SA_RS05860 cell division protein FtsZ [2] (data from MRSA252) SA_RS06125 30S ribosomal protein S16 [2] (data from MRSA252) SA_RS06140 50S ribosomal protein L19 [2] (data from MRSA252) SA_RS06165 succinyl-CoA ligase subunit beta [2] (data from MRSA252) SA_RS06170 succinyl-CoA ligase subunit alpha [2] (data from MRSA252) SA_RS06225 30S ribosomal protein S2 [2] (data from MRSA252) SA_RS06235 elongation factor Ts [2] (data from MRSA252) SA_RS06315 30S ribosomal protein S15 [2] (data from MRSA252) SA_RS06490 glutamine synthetase [2] (data from MRSA252) SA_RS06690 transketolase [2] (data from MRSA252) SA_RS06730 aconitate hydratase [2] (data from MRSA252) SA_RS07060 dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex [2] (data from MRSA252) SA_RS07065 2-oxoglutarate dehydrogenase E1 component [2] (data from MRSA252) SA_RS07120 glucose-specific phosphotransferase enzyme IIA component [2] (data from MRSA252) SA_RS07360 nucleoside-diphosphate kinase [2] (data from MRSA252) SA_RS07385 DNA-binding protein HU [2] (data from MRSA252) SA_RS07400 30S ribosomal protein S1 [2] (data from MRSA252) SA_RS07605 phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating) [2] (data from MRSA252) SA_RS07820 superoxide dismutase [2] (data from MRSA252) SA_RS07955 molecular chaperone DnaK [2] (data from MRSA252) SA_RS07985 30S ribosomal protein S20 [2] (data from MRSA252) SA_RS08435 trigger factor [2] (data from MRSA252) SA_RS08505 aldehyde dehydrogenase [2] (data from MRSA252) SA_RS08545 isocitrate dehydrogenase (NADP(+)) [2] (data from MRSA252) SA_RS08560 pyruvate kinase [2] (data from MRSA252) SA_RS08565 ATP-dependent 6-phosphofructokinase [2] (data from MRSA252) SA_RS08600 universal stress protein [2] (data from MRSA252) SA_RS08630 acetate kinase [2] (data from MRSA252) SA_RS08675 30S ribosomal protein S4 [2] (data from MRSA252) SA_RS08760 formate--tetrahydrofolate ligase [2] (data from MRSA252) SA_RS09005 transaldolase [2] (data from MRSA252) SA_RS09810 non-heme ferritin [2] (data from MRSA252) SA_RS10535 molecular chaperone GroEL [2] (data from MRSA252) SA_RS10940 beta-hydroxyacyl-ACP dehydratase [2] (data from MRSA252) SA_RS11050 50S ribosomal protein L31 type B [2] (data from MRSA252) SA_RS11075 fructose-bisphosphate aldolase [2] (data from MRSA252) SA_RS11145 purine-nucleoside phosphorylase [2] (data from MRSA252) SA_RS11245 glutamine--fructose-6-phosphate aminotransferase [2] (data from MRSA252) SA_RS11430 Asp23/Gls24 family envelope stress response protein [2] (data from MRSA252) SA_RS11600 30S ribosomal protein S9 [2] (data from MRSA252) SA_RS11605 50S ribosomal protein L13 [2] (data from MRSA252) SA_RS11630 50S ribosomal protein L17 [2] (data from MRSA252) SA_RS11640 30S ribosomal protein S11 [2] (data from MRSA252) SA_RS11645 30S ribosomal protein S13 [2] (data from MRSA252) SA_RS11660 adenylate kinase [2] (data from MRSA252) SA_RS11680 30S ribosomal protein S5 [2] (data from MRSA252) SA_RS11690 50S ribosomal protein L6 [2] (data from MRSA252) SA_RS11695 30S ribosomal protein S8 [2] (data from MRSA252) SA_RS11705 50S ribosomal protein L5 [2] (data from MRSA252) SA_RS11710 50S ribosomal protein L24 [2] (data from MRSA252) SA_RS11720 30S ribosomal protein S17 [2] (data from MRSA252) SA_RS11735 30S ribosomal protein S3 [2] (data from MRSA252) SA_RS11740 50S ribosomal protein L22 [2] (data from MRSA252) SA_RS11745 30S ribosomal protein S19 [2] (data from MRSA252) SA_RS11750 50S ribosomal protein L2 [2] (data from MRSA252) SA_RS11755 50S ribosomal protein L23 [2] (data from MRSA252) SA_RS11760 50S ribosomal protein L4 [2] (data from MRSA252) SA_RS11765 50S ribosomal protein L3 [2] (data from MRSA252) SA_RS11770 30S ribosomal protein S10 [2] (data from MRSA252) SA_RS12060 2-hydroxyacid dehydrogenase [2] (data from MRSA252) SA_RS12645 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase [2] (data from MRSA252) SA_RS13255 lactate dehydrogenase [2] (data from MRSA252) SA_RS13375 hydroxymethylglutaryl-CoA synthase [2] (data from MRSA252) SA_RS13420 L-glutamate gamma-semialdehyde dehydrogenase [2] (data from MRSA252) SA_RS13705 L-lactate dehydrogenase [2] (data from MRSA252) SA_RS13730 class I fructose-bisphosphate aldolase [2] (data from MRSA252) SA_RS13735 malate:quinone oxidoreductase [2] (data from MRSA252) SA_RS13915 ornithine carbamoyltransferase [2] (data from MRSA252) SA_RS13920 arginine deiminase [2] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
Mol Microbiol: 2002, 43(6);1387-400
[PubMed:11952893] [WorldCat.org] [DOI] (P p) - ↑ 2.000 2.001 2.002 2.003 2.004 2.005 2.006 2.007 2.008 2.009 2.010 2.011 2.012 2.013 2.014 2.015 2.016 2.017 2.018 2.019 2.020 2.021 2.022 2.023 2.024 2.025 2.026 2.027 2.028 2.029 2.030 2.031 2.032 2.033 2.034 2.035 2.036 2.037 2.038 2.039 2.040 2.041 2.042 2.043 2.044 2.045 2.046 2.047 2.048 2.049 2.050 2.051 2.052 2.053 2.054 2.055 2.056 2.057 2.058 2.059 2.060 2.061 2.062 2.063 2.064 2.065 2.066 2.067 2.068 2.069 2.070 2.071 2.072 2.073 2.074 2.075 2.076 2.077 2.078 2.079 2.080 2.081 2.082 2.083 2.084 2.085 2.086 2.087 2.088 2.089 2.090 2.091 2.092 2.093 2.094 2.095 2.096 2.097 2.098 2.099 2.100 2.101 2.102 2.103 2.104 2.105 2.106 2.107 2.108 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)