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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08560 [old locus tag: SA1520 ]
  • pan locus tag?: SAUPAN004324000
  • symbol: SA_RS08560
  • pan gene symbol?: pykA
  • synonym:
  • product: pyruvate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08560 [old locus tag: SA1520 ]
  • symbol: SA_RS08560
  • product: pyruvate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1734257..1736014
  • length: 1758
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1734257..1736014) NCBI
  • BioCyc: SA_RS08560 BioCyc
  • MicrobesOnline: see SA1520

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAGAAAAACTAAAATTGTATGTACAATTGGACCAGCTTCAGAATCAGAAGAAATGATT
    GAGAAATTAATCAATGCTGGTATGAACGTTGCACGATTAAACTTTTCACATGGTAGTCAT
    GAAGAGCATAAAGGTAGAATTGATACAATTCGTAAAGTAGCTAAAAGATTAGACAAAATT
    GTAGCAATTTTATTAGATACGAAAGGTCCAGAAATTCGTACGCATAATATGAAAGACGGT
    ATCATTGAACTTGAACGTGGTAACGAAGTTATTGTTAGCATGAATGAAGTTGAAGGAACA
    CCTGAAAAGTTCTCAGTAACATATGAAAACTTAATTAACGATGTTCAAGTAGGTTCATAC
    ATTTTACTTGATGATGGCTTAATTGAATTACAAGTTAAAGATATTGACCATGCTAAAAAA
    GAAGTTAAATGTGATATTTTAAACTCTGGTGAGCTTAAAAACAAAAAAGGTGTTAACTTA
    CCTGGTGTAAGAGTAAGTTTACCTGGTATTACAGAAAAAGATGCTGAAGATATCCGTTTC
    GGTATTAAAGAAAATGTTGACTTCATTGCAGCAAGTTTCGTACGTCGTCCTAGTGATGTT
    TTAGAAATTCGTGAAATTTTAGAAGAACAAAAAGCTAACATTTCAGTATTCCCTAAAATT
    GAAAACCAAGAAGGTATTGATAATATTGCGGAAATTCTTGAAGTGTCTGATGGTTTAATG
    GTTGCACGTGGTGACATGGGTGTTGAAATTCCACCTGAAAAAGTACCAATGGTTCAAAAA
    GATTTAATCAGACAATGTAACAAATTAGGTAAACCAGTTATTACAGCTACACAAATGTTA
    GATTCTATGCAACGTAACCCACGTGCTACACGTGCAGAAGCTAGTGACGTTGCCAACGCA
    ATCTATGATGGTACAGATGCAGTAATGTTATCTGGTGAAACTGCTGCTGGTTTATATCCT
    GAAGAAGCTGTTAAAACAATGAGAAATATTGCTGTATCAGCTGAAGCAGCCCAAGATTAT
    AAAAAGTTATTGTCAGATCGTACTAAATTAGTTGAAACTTCATTAGTGAATGCTATCGGT
    ATTTCGGTTGCACATACAGCTTTAAACTTAAATGTTAAAGCAATTGTAGCTGCTACTGAA
    AGTGGTTCAACGGCACGTACTATCTCTAAATATCGTCCACATTCAGACATTATTGCGGTG
    ACTCCAAGTGAAGAAACAGCACGTCAATGTTCAATTGTTTGGGGAGTTCAACCTGTAGTT
    AAAAAAGGACGTAAGAGTACAGATGCATTGTTAAACAATGCAGTTGCAACAGCTGTTGAA
    ACTGGTAGAGTATCTAATGGTGATTTAATCATTATTACTGCTGGTGTACCAACTGGTGAA
    ACTGGAACTACTAATATGATGAAAATCCACCTAGTTGGTGACGAAATTGCTAATGGTCAA
    GGTATTGGACGTGGATCAGTTGTTGGTACTACGTTAGTTGCTGAAACTGTTAAAGATTTA
    GAAGGTAAAGATTTATCTGACAAAGTTATCGTTACTAACTCAATCGATGAAACGTTTGTA
    CCTTATGTAGAAAAAGCTTTAGGATTAATTACAGAAGAAAATGGTATTACATCACCAAGT
    GCAATTGTTGGTTTAGAAAAAGGTATTCCAACAGTTGTAGGTGTAGAAAAAGCTGTTAAA
    AACATAAGCAATAACATGTTAGTTACGATTGATGCTGCTCAAGGTAAAATCTTTGAAGGA
    TATGCAAACGTACTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1758

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08560 [old locus tag: SA1520 ]
  • symbol: SA_RS08560
  • description: pyruvate kinase
  • length: 585
  • theoretical pI: 4.99555
  • theoretical MW: 63101.9
  • GRAVY: -0.127863

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.40?  ExPASy
    Pyruvate kinase ATP + pyruvate = ADP + phosphoenolpyruvate
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate kinase (TIGR01064; EC 2.7.1.40; HMM-score: 619.1)
    and 4 more
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphoenolpyruvate synthase (TIGR01418; EC 2.7.9.2; HMM-score: 56.3)
    phosphoenolpyruvate-protein phosphotransferase (TIGR01417; EC 2.7.3.9; HMM-score: 27.2)
    2-dehydro-3-deoxyglucarate aldolase (TIGR03239; EC 4.1.2.20; HMM-score: 15.6)
    2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (TIGR02311; EC 4.1.2.-; HMM-score: 14.4)
  • TheSEED: see SA1520
  • PFAM:
    PK_TIM (CL0151) PK; Pyruvate kinase, barrel domain (PF00224; HMM-score: 511.6)
    and 4 more
    no clan defined PK_C; Pyruvate kinase, alpha/beta domain (PF02887; HMM-score: 127.9)
    Leu-IlvD (CL0364) PEP-utilizers; PEP-utilising enzyme, mobile domain (PF00391; HMM-score: 55.7)
    HTH (CL0123) IF2_N; Translation initiation factor IF-2, N-terminal region (PF04760; HMM-score: 19)
    PK_TIM (CL0151) HpcH_HpaI; HpcH/HpaI aldolase/citrate lyase family (PF03328; HMM-score: 14.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: K+, Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012748
    • TAT(Tat/SPI): 0.000555
    • LIPO(Sec/SPII): 0.001816
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHKGRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPEKFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNLPGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFPKIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITATQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSAEAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYRPHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLIIITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDKVIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNMLVTIDAAQGKIFEGYANVL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS11130(deoA)pyrimidine-nucleoside phosphorylase  [2] (data from MRSA252)
    SA_RS00185serine--tRNA ligase  [2] (data from MRSA252)
    SA_RS00310aminoglycoside O-nucleotidyltransferase ANT(4')-Ia  [2] (data from MRSA252)
    SA_RS00690immunoglobulin G-binding protein A  [2] (data from MRSA252)
    SA_RS01275formate acetyltransferase  [2] (data from MRSA252)
    SA_RS0201530S ribosomal protein S6  [2] (data from MRSA252)
    SA_RS02090alkyl hydroperoxide reductase subunit F  [2] (data from MRSA252)
    SA_RS02145IMP dehydrogenase  [2] (data from MRSA252)
    SA_RS02150GMP synthase (glutamine-hydrolyzing)  [2] (data from MRSA252)
    SA_RS02490YbaB/EbfC family nucleoid-associated protein  [2] (data from MRSA252)
    SA_RS0265050S ribosomal protein L25/general stress protein Ctc  [2] (data from MRSA252)
    SA_RS02710cysteine synthase  [2] (data from MRSA252)
    SA_RS02810pyridoxal 5'-phosphate synthase lyase subunit PdxS  [2] (data from MRSA252)
    SA_RS0290550S ribosomal protein L11  [2] (data from MRSA252)
    SA_RS0291050S ribosomal protein L1  [2] (data from MRSA252)
    SA_RS0291550S ribosomal protein L10  [2] (data from MRSA252)
    SA_RS0292050S ribosomal protein L7/L12  [2] (data from MRSA252)
    SA_RS02930DNA-directed RNA polymerase subunit beta  [2] (data from MRSA252)
    SA_RS02955elongation factor G  [2] (data from MRSA252)
    SA_RS02960elongation factor Tu  [2] (data from MRSA252)
    SA_RS03155phosphate acetyltransferase  [2] (data from MRSA252)
    SA_RS03250zinc-dependent alcohol dehydrogenase  [2] (data from MRSA252)
    SA_RS03910ribonucleotide-diphosphate reductase subunit alpha  [2] (data from MRSA252)
    SA_RS04020ribosomal subunit interface protein  [2] (data from MRSA252)
    SA_RS04140aldehyde dehydrogenase  [2] (data from MRSA252)
    SA_RS04145phosphoglycerate kinase  [2] (data from MRSA252)
    SA_RS04150triose-phosphate isomerase  [2] (data from MRSA252)
    SA_RS04160enolase  [2] (data from MRSA252)
    SA_RS04330glycine cleavage system protein H  [2] (data from MRSA252)
    SA_RS04680glucose-6-phosphate isomerase  [2] (data from MRSA252)
    SA_RS04935hypothetical protein  [2] (data from MRSA252)
    SA_RS05295phosphocarrier protein HPr  [2] (data from MRSA252)
    SA_RS05350pyruvate dehydrogenase E1 component subunit alpha  [2] (data from MRSA252)
    SA_RS05360dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex  [2] (data from MRSA252)
    SA_RS05365dihydrolipoyl dehydrogenase  [2] (data from MRSA252)
    SA_RS05620thiol reductase thioredoxin  [2] (data from MRSA252)
    SA_RS05860cell division protein FtsZ  [2] (data from MRSA252)
    SA_RS0614050S ribosomal protein L19  [2] (data from MRSA252)
    SA_RS06165succinyl-CoA ligase subunit beta  [2] (data from MRSA252)
    SA_RS0622530S ribosomal protein S2  [2] (data from MRSA252)
    SA_RS06490glutamine synthetase  [2] (data from MRSA252)
    SA_RS06730aconitate hydratase  [2] (data from MRSA252)
    SA_RS07005cold-shock protein CspA  [2] (data from MRSA252)
    SA_RS07205serine/threonine dehydratase  [2] (data from MRSA252)
    SA_RS07210alanine dehydrogenase  [2] (data from MRSA252)
    SA_RS07605phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)  [2] (data from MRSA252)
    SA_RS07695elongation factor P  [2] (data from MRSA252)
    SA_RS07955molecular chaperone DnaK  [2] (data from MRSA252)
    SA_RS0829550S ribosomal protein L21  [2] (data from MRSA252)
    SA_RS08435trigger factor  [2] (data from MRSA252)
    SA_RS08545isocitrate dehydrogenase (NADP(+))  [2] (data from MRSA252)
    SA_RS08625universal stress protein UspA  [2] (data from MRSA252)
    SA_RS08630acetate kinase  [2] (data from MRSA252)
    SA_RS0867530S ribosomal protein S4  [2] (data from MRSA252)
    SA_RS09810non-heme ferritin  [2] (data from MRSA252)
    SA_RS11245glutamine--fructose-6-phosphate aminotransferase  [2] (data from MRSA252)
    SA_RS11430Asp23/Gls24 family envelope stress response protein  [2] (data from MRSA252)
    SA_RS1160030S ribosomal protein S9  [2] (data from MRSA252)
    SA_RS11635DNA-directed RNA polymerase subunit alpha  [2] (data from MRSA252)
    SA_RS1164030S ribosomal protein S11  [2] (data from MRSA252)
    SA_RS1164530S ribosomal protein S13  [2] (data from MRSA252)
    SA_RS1167050S ribosomal protein L15  [2] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [2] (data from MRSA252)
    SA_RS1169050S ribosomal protein L6  [2] (data from MRSA252)
    SA_RS1170550S ribosomal protein L5  [2] (data from MRSA252)
    SA_RS1172030S ribosomal protein S17  [2] (data from MRSA252)
    SA_RS1173050S ribosomal protein L16  [2] (data from MRSA252)
    SA_RS1173530S ribosomal protein S3  [2] (data from MRSA252)
    SA_RS1174050S ribosomal protein L22  [2] (data from MRSA252)
    SA_RS1175050S ribosomal protein L2  [2] (data from MRSA252)
    SA_RS1175550S ribosomal protein L23  [2] (data from MRSA252)
    SA_RS13420L-glutamate gamma-semialdehyde dehydrogenase  [2] (data from MRSA252)
    SA_RS13705L-lactate dehydrogenase  [2] (data from MRSA252)
    SA_RS13730class I fructose-bisphosphate aldolase  [2] (data from MRSA252)
    SA_RS13735malate:quinone oxidoreductase  [2] (data from MRSA252)
    SA_RS13915ornithine carbamoyltransferase  [2] (data from MRSA252)
    SA_RS13920arginine deiminase  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 2.36 2.37 2.38 2.39 2.40 2.41 2.42 2.43 2.44 2.45 2.46 2.47 2.48 2.49 2.50 2.51 2.52 2.53 2.54 2.55 2.56 2.57 2.58 2.59 2.60 2.61 2.62 2.63 2.64 2.65 2.66 2.67 2.68 2.69 2.70 2.71 2.72 2.73 2.74 2.75 2.76 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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