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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001682
  • pan locus tag?: SAUPAN004324000
  • symbol: pyk
  • pan gene symbol?: pykA
  • synonym:
  • product: pyruvate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001682
  • symbol: pyk
  • product: pyruvate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1720417..1722174
  • length: 1758
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAGAAAAACTAAAATTGTATGTACAATTGGACCAGCTTCAGAATCAGAAGAAATGATT
    GAGAAATTAATCAATGCTGGTATGAACGTTGCACGATTAAACTTTTCACATGGTAGTCAT
    GAAGAGCATAAAGGTAGAATTGATACAATTCGTAAAGTAGCTAAAAGATTAGACAAAATT
    GTAGCAATTTTATTAGATACAAAAGGTCCAGAAATTCGTACGCATAATATGAAAGACGGT
    ATCATTGAACTTGAACGTGGTAACGAAGTTATTGTTAGCATGAATGAAGTTGAAGGAACA
    CCTGAAAAGTTCTCAGTAACATATGAAAACTTAATTAACGATGTTCAAGTAGGTTCATAC
    ATTTTACTTGATGATGGCTTAATTGAATTACAAGTTAAAGATATTGACCATGCTAAAAAA
    GAAGTTAAATGTGATATTTTAAACTCTGGTGAGCTTAAAAACAAAAAAGGTGTTAACTTA
    CCTGGCGTAAGAGTAAGTTTACCTGGTATTACAGAAAAAGATGCTGAAGATATCCGTTTC
    GGTATTAAAGAAAATGTTGACTTCATTGCAGCAAGTTTCGTACGTCGTCCTAGTGATGTT
    TTAGAAATTCGTGAAATTTTAGAAGAACAAAAAGCTAACATTTCAGTATTCCCTAAAATT
    GAAAACCAAGAAGGTATTGATAATATTGCGGAAATTCTTGAAGTGTCTGATGGTTTAATG
    GTTGCACGTGGTGACATGGGTGTTGAAATTCCACCTGAAAAAGTACCAATGGTTCAAAAA
    GATTTAATCAGACAATGTAACAAATTAGGTAAACCAGTTATTACAGCTACACAAATGTTA
    GATTCTATGCAACGTAACCCACGTGCTACACGTGCAGAAGCTAGTGACGTTGCCAACGCA
    ATCTATGATGGTACAGATGCAGTAATGTTATCTGGTGAAACTGCTGCTGGTTTATATCCT
    GAAGAAGCTGTTAAAACAATGAGAAATATTGCTGTATCAGCTGAAGCAGCCCAAGATTAC
    AAAAAGTTATTGTCAGATCGTACTAAATTAGTTGAAACTTCATTAGTGAATGCTATCGGT
    ATTTCGGTTGCACATACAGCTTTAAACTTAAATGTTAAAGCAATTGTAGCTGCTACTGAA
    AGTGGTTCAACGGCACGTACTATCTCTAAATATCGTCCACATTCAGACATTATTGCGGTG
    ACTCCAAGTGAAGAAACTGCACGTCAATGTTCAATTGTTTGGGGAGTTCAACCTGTAGTT
    AAAAAAGGACGTAAGAGTACAGATGCATTGTTAAACAATGCAGTTGCAACAGCTGTTGAA
    ACTGGTAGAGTATCTAATGGTGATTTAATCATTATTACTGCTGGTGTACCAACTGGTGAA
    ACTGGAACTACTAATATGATGAAAATCCACCTAGTTGGTGACGAAATTGCTAATGGTCAA
    GGTATTGGACGTGGATCAGTTGTTGGTACTACGTTAGTTGCTGAAACTGTTAAAGATTTA
    GAAGGTAAAGATTTATCTGACAAAGTTATCGTTACTAACTCAATCGATGAAACGTTTGTA
    CCTTATGTAGAAAAAGCTTTAGGCTTAATTACAGAAGAAAATGGTATTACATCACCAAGT
    GCAATTGTTGGTTTAGAAAAAGGTATTCCAACAGTTGTAGGTGTAGAAAAAGCTGTTAAA
    AACATAAGCAATAACATGTTAGTTACGATTGATGCTGCTCAAGGTAAAATCTTTGAAGGA
    TATGCAAACGTACTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1758

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001682
  • symbol: Pyk
  • description: pyruvate kinase
  • length: 585
  • theoretical pI: 4.99555
  • theoretical MW: 63101.9
  • GRAVY: -0.127863

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.40?  ExPASy
    Pyruvate kinase ATP + pyruvate = ADP + phosphoenolpyruvate
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate kinase (TIGR01064; EC 2.7.1.40; HMM-score: 619.1)
    and 4 more
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphoenolpyruvate synthase (TIGR01418; EC 2.7.9.2; HMM-score: 56.3)
    phosphoenolpyruvate-protein phosphotransferase (TIGR01417; EC 2.7.3.9; HMM-score: 27.2)
    2-dehydro-3-deoxyglucarate aldolase (TIGR03239; EC 4.1.2.20; HMM-score: 15.6)
    2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (TIGR02311; EC 4.1.2.-; HMM-score: 14.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) PK; Pyruvate kinase, barrel domain (PF00224; HMM-score: 492.9)
    and 6 more
    no clan defined PK_C; Pyruvate kinase, alpha/beta domain (PF02887; HMM-score: 137.2)
    Leu-IlvD (CL0364) PEP-utilizers; PEP-utilising enzyme, mobile domain (PF00391; HMM-score: 61.7)
    HTH (CL0123) IF2_N; Translation initiation factor IF-2, N-terminal region (PF04760; HMM-score: 18.4)
    TIM_barrel (CL0036) HpcH_HpaI; HpcH/HpaI aldolase/citrate lyase (PF03328; HMM-score: 14.2)
    Cellulase; Cellulase (glycosyl hydrolase family 5) (PF00150; HMM-score: 13)
    Mur_ligase_C_sf (CL0794) Mur_ligase_C; Mur ligase, glutamate ligase domain (PF02875; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9063
    • Cytoplasmic Membrane Score: 0.0119
    • Cell wall & surface Score: 0.0015
    • Extracellular Score: 0.0803
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012748
    • TAT(Tat/SPI): 0.000555
    • LIPO(Sec/SPII): 0.001816
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHKGRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPEKFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNLPGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFPKIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITATQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSAEAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYRPHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLIIITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDKVIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNMLVTIDAAQGKIFEGYANVL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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