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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08760 [old locus tag: SA1553 ]
  • pan locus tag?: SAUPAN004396000
  • symbol: SA_RS08760
  • pan gene symbol?: fhs
  • synonym:
  • product: formate--tetrahydrofolate ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08760 [old locus tag: SA1553 ]
  • symbol: SA_RS08760
  • product: formate--tetrahydrofolate ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1778288..1779955
  • length: 1668
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1778288..1779955) NCBI
  • BioCyc: SA_RS08760 BioCyc
  • MicrobesOnline: see SA1553

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
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    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    TTGACTCATTTATCAGATTTAGATATTGCGAATCAATCAACACTACAACCAATTAAGGAT
    ATTGCTGCATCAGTAGGTATTTCAGAGGATGCATTAGAACCTTATGGTCATTACAAAGCT
    AAAATCGACATTAATAAAATTACGCCAAGAGAAAACAAAGGGAAAGTTGTTTTAGTAACT
    GCGATGAGCCCAACACCAGCTGGTGAAGGTAAATCAACGGTTACAGTTGGTTTAGCTGAT
    GCATTCCATGAGTTAAATAAAAACGTTATGGTTGCATTAAGAGAGCCTGCTTTAGGACCA
    ACATTTGGTATCAAAGGTGGTGCGACTGGTGGTGGTTATGCGCAAGTCTTACCTATGGAA
    GATATCAACTTACATTTCAACGGAGATTTCCATGCGATTACAACTGCAAATAATGCATTG
    TCTGCGTTTATAGATAATCATATTCACCAAGGTAATGAATTAGGAATCGATCAAAGACGT
    ATTGAATGGAAACGTGTATTAGATATGAATGATCGTGCACTTAGACATGTAAACGTTGGG
    TTAGGTGGACCTACAAATGGTGTACCACGTGAAGATGGCTTTAATATTACAGTAGCGTCT
    GAAATTATGGCGATTTTATGTTTAAGTAGAAGTATTAAAGACTTAAAAGATAAAATTAGT
    CGTATTACTATTGGTTACACTAGAGATCGCAAGCCAGTTACAGTTGCAGATTTAAAAGTG
    CAAGGTGCACTTGCAATGATTTTAAAAGATGCAATTAAACCAAACTTAGTACAATCAATT
    GAAGGGACACCTGCATTAGTTCATGGTGGACCATTTGCGAATATCGCACACGGTTGTAAC
    TCAATTTTAGCAACTGAAACAGCACGTGATTTAGCTGATATCGTTGTAACGGAAGCTGGA
    TTTGGTTCAGACTTAGGCGCTGAAAAATTCATGGACATTAAAGCGCGTGAAGCAGGATTT
    GATCTGGCAGCTGTCGTTGTTGTTGCGACAATTCGTGCGTTAAAAATGCATGGTGGTGTA
    GCGAAAGATAATTTAAAAGAAGAAAATGTAGAAGCAGTAAAAGCAGGAATTGTTAATTTA
    GAGCGTCATGTTAATAATATTAAAAAATTCGGTGTAGAACCGGTTGTTGCAATTAATGCA
    TTTATACATGATACCGATGCAGAAGTAGAATATGTAAAATCTTGGGCTAAAGAAAATAAC
    GTACGAATTGCCTTAACTGAAGTTTGGGAAAAAGGTGGTAAAGGTGGCGTTGACTTAGCA
    AATGAAGTATTAGAAGTCATTGATCAACCTAATTCATTTAAACCTTTATATGAATTAGAA
    TTACCATTAGAGCAAAAGATTGAAAAGATTGTGACTGAAATCTATGGCGGTTCAAAAGTA
    ACGTTTAGCAGTAAAGCGCAAAAACAATTAAAACAATTTAAAGAAAATGGTTGGGATAAT
    TACCCAGTATGTATGGCGAAAACACAATATTCATTCTCAGATGATCAAACGTTGTTAGGT
    GCACCATCAGGATTTGAAATTACAATTCGTGAATTAGAAGCGAAAACAGGTGCAGGATTT
    ATCGTAGCGTTGACAGGTGCAATCATGACTATGCCTGGTTTACCTAAAAAACCAGCAGCA
    TTAAACATGGATGTTACTGATGATGGTCATGCAATTGGGTTATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1668

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08760 [old locus tag: SA1553 ]
  • symbol: SA_RS08760
  • description: formate--tetrahydrofolate ligase
  • length: 555
  • theoretical pI: 5.91333
  • theoretical MW: 59871.1
  • GRAVY: -0.105405

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.4.3?  ExPASy
    Formate--tetrahydrofolate ligase ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
  • TIGRFAM:
  • TheSEED: see SA1553
  • PFAM:
    P-loop_NTPase (CL0023) FTHFS; Formate--tetrahydrofolate ligase (PF01268; HMM-score: 878.3)
    and 2 more
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 16.1)
    POTRA (CL0191) POTRA_TamA_1; POTRA domain TamA domain 1 (PF17243; HMM-score: 16)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005497
    • TAT(Tat/SPI): 0.001617
    • LIPO(Sec/SPII): 0.001074
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDINKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTFGIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRRIEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDKISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIAHGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDLAAVVVVATIRALKMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYVKSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKIVTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEITIRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS02960elongation factor Tu  [1] (data from MRSA252)
    SA_RS05350pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA_RS05360dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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