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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1782 [new locus tag: SACOL_RS09125 ]
  • pan locus tag?: SAUPAN004396000
  • symbol: fhs
  • pan gene symbol?: fhs
  • synonym:
  • product: formate--tetrahydrofolate ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1782 [new locus tag: SACOL_RS09125 ]
  • symbol: fhs
  • product: formate--tetrahydrofolate ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1824399..1826066
  • length: 1668
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    TTGACTCATTTATCAGATTTAGATATTGCGAATCAATCAACACTACAACCAATTAAGGAT
    ATTGCTGCATCAGTAGGTATTTCAGAGGATGCATTAGAACCTTATGGTCATTACAAAGCT
    AAAATCGACATTAATAAAATTACGCCAAGAGAAAACAAAGGGAAAGTTGTTTTAGTAACT
    GCGATGAGCCCAACACCAGCTGGTGAAGGTAAATCAACGGTTACAGTTGGTTTAGCTGAT
    GCATTCCATGAGTTAAATAAAAACGTTATGGTTGCATTAAGAGAGCCTGCTTTAGGACCA
    ACATTTGGTATCAAAGGTGGTGCGACTGGTGGTGGTTATGCGCAAGTCTTACCTATGGAA
    GATATCAACTTACATTTCAACGGAGATTTCCATGCGATTACAACTGCAAATAATGCATTG
    TCTGCGTTTATCGATAATCATATTCACCAAGGTAACGAATTAGGAATCGATCAAAGACGT
    ATTGAGTGGAAACGTGTATTAGATATGAATGATCGTGCACTTAGACATGTAAACGTTGGG
    TTAGGTGGACCTACAAATGGTGTACCACGTGAAGATGGCTTTAATATTACAGTAGCGTCT
    GAAATTATGGCGATTTTATGTTTAAGTAGAAGTATTAAAGACTTAAAAGATAAAATTAGT
    CGTATTACTATTGGTTACACTAGAGATCGCAAGCCAGTTACAGTTGCAGATTTAAAAGTG
    GAAGGTGCACTTGCAATGATTTTAAAAGATGCAATAAAACCAAACTTAGTACAATCAATT
    GAAGGGACACCTACATTAGTTCATGGTGGACCATTTGCGAATATCGCACACGGTTGTAAC
    TCAATTTTAGCAACTGAAACAGCACGTGATTTAGCTGATATCGTTGTAACGGAAGCTGGA
    TTTGGTTCAGACTTAGGCGCTGAAAAATTCATGGACATTAAAGTGCGTGAAGCAGGATTT
    GATCCGGCAGCTGTCGTTGTTGTTGCGACAATTCGTGCGTTAAAAATGCATGGTGGTGTA
    GCGAAAGATAATTTAAAAGAAGAAAATGTAGAAGCAGTAAAAGCAGGAATTGTTAATTTA
    GAGCGTCATGTTAATAATATTAAAAAATTCGGTGTAGAACCGGTTGTTGCAATTAATGCA
    TTTATACATGATACCGATGCAGAAGTAGAATATGTAAAATCTTGGGCTAAAGAAAATAAC
    GTACGAATTGCCTTAACTGAAGTTTGGAAAAAAGGTGGTAAAGGTGGCGTTGACTTAGCA
    AATGAAGTATTAGAAGTCATTGATCAACCTAATTCATTTAAACCTTTATATGAATTAGAA
    TTACCATTAGAGCAAAAGATTGAAAAGATTGTGACTGAAATCTATGGCGGTTCAAAAGTA
    ACGTTTAGCAGTAAAGCGCAAAAACAATTAAAACAATTTAAAGAAAATGGTTGGGATAAT
    TACCCAGTATGTATGGCGAAAACACAATATTCATTCTCAGATGATCAAACGTTGTTAGGT
    GCACCATCAGGATTTGAAATTACAATTCGTGAATTAGAAGCGAAAACAGGTGCAGGATTT
    ATCGTAGCGTTGACAGGTGCAATCATGACTATGCCTGGTTTACCTAAAAAACCAGCAGCA
    TTAAACATGGATGTTACTGATGATGGTCATGCAATTGGGTTATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1668

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1782 [new locus tag: SACOL_RS09125 ]
  • symbol: Fhs
  • description: formate--tetrahydrofolate ligase
  • length: 555
  • theoretical pI: 6.04634
  • theoretical MW: 59913.2
  • GRAVY: -0.116036

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.4.3?  ExPASy
    Formate--tetrahydrofolate ligase ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
  • TIGRFAM:
  • TheSEED  :
    • Formate--tetrahydrofolate ligase (EC 6.3.4.3)
    Carbohydrates One-carbon Metabolism One-carbon metabolism by tetrahydropterines  Formate--tetrahydrofolate ligase (EC 6.3.4.3)
    and 3 more
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Formate--tetrahydrofolate ligase (EC 6.3.4.3)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Formate--tetrahydrofolate ligase (EC 6.3.4.3)
    Miscellaneous Miscellaneous - no subcategory EC 6.3.4.- Ligases that form carbon-nitrogen bonds  Formate--tetrahydrofolate ligase (EC 6.3.4.3)
  • PFAM:
    P-loop_NTPase (CL0023) FTHFS; Formate--tetrahydrofolate ligase (PF01268; HMM-score: 880.6)
    and 2 more
    POTRA (CL0191) POTRA_TamA_1; POTRA domain TamA domain 1 (PF17243; HMM-score: 15.9)
    P-loop_NTPase (CL0023) CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005497
    • TAT(Tat/SPI): 0.001617
    • LIPO(Sec/SPII): 0.001074
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDINKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTFGIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRRIEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDKISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPTLVHGGPFANIAHGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRALKMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYVKSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKIVTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEITIRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 2017 [5]
  • interaction partners:
    SACOL1102(pdhA)pyruvate dehydrogenase complex E1 component subunit alpha  [6] (data from MRSA252)
    SACOL1104(pdhC)branched-chain alpha-keto acid dehydrogenase E2  [6] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [6] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 83.11 h [7]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ 6.0 6.1 6.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  7. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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