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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_0453 [new locus tag: NWMN_RS02590 ]
- pan locus tag?: SAUPAN002227000
- symbol: metS
- pan gene symbol?: metS
- synonym:
- product: methionyl-tRNA synthetase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_0453 [new locus tag: NWMN_RS02590 ]
- symbol: metS
- product: methionyl-tRNA synthetase
- replicon: chromosome
- strand: +
- coordinates: 515662..517635
- length: 1974
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5330245 NCBI
- RefSeq: YP_001331487 NCBI
- BioCyc:
- MicrobesOnline: 3705987 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1921ATGGCTAAAGAAACATTTTATATAACAACCCCAATATACTATCCTAGTGGGAATTTACAT
ATAGGACATGCATATTCTACAGTGGCTGGAGATGTTATTGCAAGATATAAGAGAATGCAA
GGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAA
GCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAA
CAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGAACAACTGAAGAACGT
CATAAACATGTCGTTGAGCAAGTGTTTGAACGTTTATTAAAGCAAGGTGATATCTATTTA
GGTGAATATGAAGGTTGGTATTCTGTTCCGGATGAAACATACTATACAGAGTCACAATTA
GTAGACCCACAATACGAAAACGGTAAAATTATTGGTGGCAAAAGTCCAGATTCTGGACAC
GAAGTTGAACTAGTTAAAGAAGAAAGTTATTTCTTTAATATTAGTAAATATACAGACCGT
TTATTAGAGTTCTATGACCAAAATCCAGATTTTATACAACCACCATCAAGAAAAAATGAA
ATGATTAACAACTTCATTAAACCAGGACTTGCTGATTTAGCTGTTTCTCGTACATCATTT
AACTGGGGTGTCCATGTTCCGTCTAATCCAAAACATGTTGTTTATGTTTGGATTGATGCG
TTAGTTAACTATATTTCAGCATTAGGCTATTTATCAGATGATGAGTCACTATTTAACAAA
TACTGGCCAGCAGATATTCATTTAATGGCTAAGGAAATTGTGCGATTCCACTCAATTATT
TGGCCTATTTTATTGATGGCATTAGACTTACCGTTACCTAAAAAAGTCTTTGCACATGGT
TGGATTTTGATGAAAGATGGAAAAATGAGTAAATCTAAAGGTAATGTCGTAGACCCTAAT
ATTTTAATTGATCGCTATGGTTTAGATGCTACACGTTATTATCTAATGCGTGAATTACCA
TTTGGTTCAGATGGCGTATTTACACCTGAAGCATTTGTTGAGCGTACAAATTTCGATCTA
GCAAATGACTTAGGTAACTTAGTAAACCGTACGATTTCTATGGTTAATAAGTACTTTGAT
GGCGAATTACCAGCGTATCAAGGTCCACTTCATGAATTAGATGAAGAAATGGAAGCTATG
GCTTTAGAAACAGTGAAAAGCTACACTGAAAGCATGGAAAGTTTGCAATTTTCTGTGGCA
TTATCTACGGTATGGAAGTTTATTAGTAGAACGAATAAGTATATTGACGAAACAACGCCT
TGGGTATTAGCTAAGGACGATAGCCAAAAAGATATGTTAGGCAATGTAATGGCTCACTTA
GTTGAAAATATTCGTTATGCAGCTGTATTATTACGTCCATTCTTAACACATGCGCCGAAA
GAGATTTTTGAACAATTGAACATTAACAATCCTCAATTTATGGAATTTAGTAGTTTAGAG
CAATATGGTGTGCTTAATGAGTCAATTATGGTTACTGGGCAACCTAAACCTATTTTCCCA
AGATTGGATAGCGAAGCGGAAATTGCATATATCAAAGAATCAATGCAACCGCCTGCTACT
AAAGAGGAAAAAGAAGAGATTCCTAGCAAACCTCAAATTGATATTAAAGACTTTGATAAA
GTTGAAATTAAGGCAGCAACGATTATTGATGCTGAACATGTTAAGAAGTCAGATAAGCTT
TTAAAAATTCAAGTAGACTTAGATTCTGAACAAAGACAAATTGTATCAGGAATTGCCAAA
TTCTATACACCAGATGATATTATTGGTAAAAAAGTAGCAGTTGTTACTAACCTGAAACCA
GCTAAATTAATGGGACAAAAATCTGAAGGTATGATATTATCTGCTGAAAAAGATGGTGTA
TTAACCTTAGTAAGTTTACCAAGTGCAATTCCAAATGGTGCAGTGATTAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_0453 [new locus tag: NWMN_RS02590 ]
- symbol: MetS
- description: methionyl-tRNA synthetase
- length: 657
- theoretical pI: 4.94244
- theoretical MW: 74885
- GRAVY: -0.338661
⊟Function[edit | edit source]
- reaction: EC 6.1.1.10? ExPASyMethionine--tRNA ligase ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
- TIGRFAM: Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 564.6)and 12 moreProtein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 149)Protein synthesis tRNA aminoacylation methionine--tRNA ligase, beta subunit (TIGR00399; EC 6.1.1.10; HMM-score: 138.6)Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 114.5)Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 112.1)Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 81.2)Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 62)Protein fate Protein and peptide secretion and trafficking export-related chaperone protein CsaA (TIGR02222; HMM-score: 38.7)Protein fate Protein folding and stabilization export-related chaperone protein CsaA (TIGR02222; HMM-score: 38.7)Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, beta subunit (TIGR00472; EC 6.1.1.20; HMM-score: 27.2)Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 26.2)Protein synthesis tRNA aminoacylation arginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 23.8)Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 16.4)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 387.2)and 10 moretRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 105.5)OB (CL0021) tRNA_bind; Putative tRNA binding domain (PF01588; HMM-score: 89.1)HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 56.5)DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA (PF08264; HMM-score: 36.3)HUP (CL0039) tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 25.3)Pilus (CL0327) PilM; PilM (PF07419; HMM-score: 14.6)no clan defined ARL2_Bind_BART; The ARF-like 2 binding protein BART (PF11527; HMM-score: 12.9)HUP (CL0039) tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 12.6)no clan defined T4_neck-protein; Virus neck protein (PF11649; HMM-score: 10.8)HUP (CL0039) tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 9.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.014505
- TAT(Tat/SPI): 0.001142
- LIPO(Sec/SPII): 0.001179
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDVRYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHKHVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGHEVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRTSFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRFHSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYYLMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHELDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQKDMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNESIMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAATIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLMGQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
NWMN_1605 (ackA) acetate kinase [1] (data from MRSA252) NWMN_2131 (adk) adenylate kinase [1] (data from MRSA252) NWMN_0372 (ahpC) alkyl hydroperoxide reductase subunit C [1] (data from MRSA252) NWMN_2534 (arcA) arginine deiminase [1] (data from MRSA252) NWMN_2533 (arcB) ornithine carbamoyltransferase [1] (data from MRSA252) NWMN_1587 (citC) isocitrate dehydrogenase [1] (data from MRSA252) NWMN_2042 (deoD) purine nucleoside phosphorylase [1] (data from MRSA252) NWMN_1483 (dnaK) molecular chaperone DnaK [1] (data from MRSA252) NWMN_0745 (eno) phosphopyruvate hydratase [1] (data from MRSA252) NWMN_0551 (eutD) phosphotransacetylase [1] (data from MRSA252) NWMN_2003 (fabZ) (3R)-hydroxymyristoyl-ACP dehydratase [1] (data from MRSA252) NWMN_2029 (fbaA) fructose-bisphosphate aldolase [1] (data from MRSA252) NWMN_1625 (fhs) formate--tetrahydrofolate ligase [1] (data from MRSA252) NWMN_1096 (ftsZ) cell division protein FtsZ [1] (data from MRSA252) NWMN_0509 (fus) elongation factor G [1] (data from MRSA252) NWMN_0741 (gapA) glyceraldehyde 3-phosphate dehydrogenase 1 [1] (data from MRSA252) NWMN_1580 (gapB) glyceraldehyde 3-phosphate dehydrogenase 2 [1] (data from MRSA252) NWMN_1838 (gatA) aspartyl/glutamyl-tRNA amidotransferase subunit A [1] (data from MRSA252) NWMN_2056 (glmS) glucosamine--fructose-6-phosphate aminotransferase [1] (data from MRSA252) NWMN_1217 (glnA) glutamine synthetase [1] (data from MRSA252) NWMN_1417 (gnd) 6-phosphogluconate dehydrogenase [1] (data from MRSA252) NWMN_2315 (gpmA) phosphoglyceromutase [1] (data from MRSA252) NWMN_1937 (groEL) chaperonin GroEL [1] (data from MRSA252) NWMN_0380 (guaB) inosine-5'-monophosphate dehydrogenase [1] (data from MRSA252) NWMN_0828 (gudB) NAD-specific glutamate dehydrogenase [1] (data from MRSA252) NWMN_2499 (ldh) L-lactate dehydrogenase 2 [1] (data from MRSA252) NWMN_0176 (ldh1) L-lactate dehydrogenase [1] (data from MRSA252) NWMN_2446 (mvaS) 3-hydroxy-3-methylglutaryl coenzyme A synthase [1] (data from MRSA252) NWMN_1378 (ndk) nucleoside diphosphate kinase [1] (data from MRSA252) NWMN_0962 (pdhD) dihydrolipoamide dehydrogenase [1] (data from MRSA252) NWMN_2040 (pdp) pyrimidine-nucleoside phosphorylase [1] (data from MRSA252) NWMN_1593 (pfk) 6-phosphofructokinase [1] (data from MRSA252) NWMN_0162 (pflB) formate acetyltransferase [1] (data from MRSA252) NWMN_0833 (pgi) glucose-6-phosphate isomerase [1] (data from MRSA252) NWMN_0742 (pgk) phosphoglycerate kinase [1] (data from MRSA252) NWMN_0744 (pgm) phosphoglyceromutase [1] (data from MRSA252) NWMN_0959 (phdA) pyruvate dehydrogenase E1 component, alpha subunit [1] (data from MRSA252) NWMN_0463 (prs) ribose-phosphate pyrophosphokinase [1] (data from MRSA252) NWMN_1592 (pykA) pyruvate kinase [1] (data from MRSA252) NWMN_0500 (rplA) 50S ribosomal protein L1 [1] (data from MRSA252) NWMN_2149 (rplB) 50S ribosomal protein L2 [1] (data from MRSA252) NWMN_2152 (rplC) 50S ribosomal protein L3 [1] (data from MRSA252) NWMN_2151 (rplD) 50S ribosomal protein L4 [1] (data from MRSA252) NWMN_2140 (rplE) 50S ribosomal protein L5 [1] (data from MRSA252) NWMN_2137 (rplF) 50S ribosomal protein L6 [1] (data from MRSA252) NWMN_0014 (rplI) 50S ribosomal protein L9 [1] (data from MRSA252) NWMN_0501 (rplJ) 50S ribosomal protein L10 [1] (data from MRSA252) NWMN_0499 (rplK) 50S ribosomal protein L11 [1] (data from MRSA252) NWMN_2120 (rplM) 50S ribosomal protein L13 [1] (data from MRSA252) NWMN_2125 (rplQ) 50S ribosomal protein L17 [1] (data from MRSA252) NWMN_1151 (rplS) 50S ribosomal protein L19 [1] (data from MRSA252) NWMN_2147 (rplV) 50S ribosomal protein L22 [1] (data from MRSA252) NWMN_2150 (rplW) 50S ribosomal protein L23 [1] (data from MRSA252) NWMN_2141 (rplX) 50S ribosomal protein L24 [1] (data from MRSA252) NWMN_0464 (rplY) 50S ribosomal protein L25/general stress protein Ctc [1] (data from MRSA252) NWMN_2024 (rpmE2) 50S ribosomal protein L31 type B [1] (data from MRSA252) NWMN_0505 (rpoC) DNA-directed RNA polymerase subunit beta' [1] (data from MRSA252) NWMN_1385 (rpsA) 30S ribosomal protein S1 [1] (data from MRSA252) NWMN_1166 (rpsB) 30S ribosomal protein S2 [1] (data from MRSA252) NWMN_2146 (rpsC) 30S ribosomal protein S3 [1] (data from MRSA252) NWMN_1613 (rpsD) 30S ribosomal protein S4 [1] (data from MRSA252) NWMN_2135 (rpsE) 30S ribosomal protein S5 [1] (data from MRSA252) NWMN_0357 (rpsF) 30S ribosomal protein S6 [1] (data from MRSA252) NWMN_0508 (rpsG) 30S ribosomal protein S7 [1] (data from MRSA252) NWMN_2138 (rpsH) 30S ribosomal protein S8 [1] (data from MRSA252) NWMN_2119 (rpsI) 30S ribosomal protein S9 [1] (data from MRSA252) NWMN_2153 (rpsJ) 30S ribosomal protein S10 [1] (data from MRSA252) NWMN_2127 (rpsK) 30S ribosomal protein S11 [1] (data from MRSA252) NWMN_2128 (rpsM) 30S ribosomal protein S13 [1] (data from MRSA252) NWMN_1182 (rpsO) 30S ribosomal protein S15 [1] (data from MRSA252) NWMN_1148 (rpsP) 30S ribosomal protein S16 [1] (data from MRSA252) NWMN_2143 (rpsQ) 30S ribosomal protein S17 [1] (data from MRSA252) NWMN_0359 (rpsR) 30S ribosomal protein S18 [1] (data from MRSA252) NWMN_2148 (rpsS) 30S ribosomal protein S19 [1] (data from MRSA252) NWMN_1488 (rpsT) 30S ribosomal protein S20 [1] (data from MRSA252) NWMN_0008 (serS) seryl-tRNA synthetase [1] (data from MRSA252) NWMN_1456 (sodA) superoxide dismutase Mn/Fe family protein [1] (data from MRSA252) NWMN_1326 (sucA) 2-oxoglutarate dehydrogenase E1 component [1] (data from MRSA252) NWMN_1325 (sucB) dihydrolipoamide succinyltransferase [1] (data from MRSA252) NWMN_1155 (sucC) succinyl-CoA synthetase subunit beta [1] (data from MRSA252) NWMN_1156 (sucD) succinyl-CoA synthetase subunit alpha [1] (data from MRSA252) NWMN_1569 (tig) trigger factor [1] (data from MRSA252) NWMN_1254 (tkt) transketolase [1] (data from MRSA252) NWMN_0743 (tpiA) triosephosphate isomerase [1] (data from MRSA252) NWMN_1057 (trxA) thioredoxin [1] (data from MRSA252) NWMN_1167 (tsf) elongation factor Ts [1] (data from MRSA252) NWMN_0510 (tufA) elongation factor Tu [1] (data from MRSA252) NWMN_0346 acetyl-CoA acetyltransferase [1] (data from MRSA252) NWMN_0428 ABC transporter substrate-binding protein [1] (data from MRSA252) NWMN_0443 hypothetical protein [1] (data from MRSA252) NWMN_0475 cysteine synthase-like protein [1] (data from MRSA252) NWMN_0481 pyridoxal biosynthesis lyase PdxS [1] (data from MRSA252) NWMN_0601 hypothetical protein [1] (data from MRSA252) NWMN_0811 hypothetical protein [1] (data from MRSA252) NWMN_0839 fumarylacetoacetate hydrolase family protein [1] (data from MRSA252) NWMN_0870 oligoendopeptidase F [1] (data from MRSA252) NWMN_1263 aconitate hydratase [1] (data from MRSA252) NWMN_1333 phosphotransferase system glucose-specific IIA component [1] (data from MRSA252) NWMN_1382 DNA-binding protein HU [1] (data from MRSA252) NWMN_1600 universal stress protein family protein [1] (data from MRSA252) NWMN_1672 putative translaldolase [1] (data from MRSA252) NWMN_1831 ferritin [1] (data from MRSA252) NWMN_2086 alkaline shock protein 23 [1] (data from MRSA252) NWMN_2205 D-isomer specific 2-hydroxyacid dehydrogenase family protein [1] (data from MRSA252) NWMN_2422 D-lactate dehydrogenase [1] (data from MRSA252) NWMN_2454 1-pyrroline-5-carboxylate dehydrogenase [1] (data from MRSA252) NWMN_2503 fructose-1,6-bisphosphate aldolase [1] (data from MRSA252) NWMN_2504 malate:quinone oxidoreductase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)