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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1605 [new locus tag: NWMN_RS09000 ]
  • pan locus tag?: SAUPAN004363000
  • symbol: ackA
  • pan gene symbol?: ackA
  • synonym:
  • product: acetate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1605 [new locus tag: NWMN_RS09000 ]
  • symbol: ackA
  • product: acetate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1782338..1783540
  • length: 1203
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTCAAAATTAATCTTGGCTATCAATGCTGGTAGTTCATCATTAAAATTTCAATTAATT
    AGAATGCCTGAAGAGGAATTAGTAACAAAAGGTTTAGTAGAAAGAATCGGATTGAAGGAT
    TCAATTTTTACAATTGAAGTCAACGGGGAAAAAGTTAAAACAGTACAAGATATCAAGGAT
    CACGTTGAGGCAGTTGATATCATGTTGGATGCTTTTAAAGCGCACAATATTATTAATGAT
    ATTAACGATATCGATGGAACAGGTCACCGTGTTGTACATGGTGGTGAAAAATTCCCTGAA
    TCAGTAGCTATTACTGATGAGGTTGAAAAGGAAATTGAAGAATTAAGCGAATTAGCACCG
    CTTCACAACCCAGCTAACTTGATGGGTATTCGTGCTTTCCGTAAATTATTACCAAATATC
    CCTCATGTGGCAATTTTTGATACAGCATTCCATCAAACAATGCCTGAAAAAGCATATCTA
    TATAGCTTGCCATATCATTATTATAAAGATTATGGCATTCGTAAGTATGGTTTCCATGGT
    ACAAGCCATAAATTTGTATCACAAAGAGCGGCAGAAATGTTAGATAAGCCAATTGAAGAT
    TTACGTATTATTTCATGTCACATTGGTAATGGAGCATCAATTGCAGCTATCGATGGCGGT
    AAATCTATTGATACATCAATGGGCTTCACACCACTTGCTGGTGTAACAATGGGTACTCGT
    TCTGGTAATATTGACCCTGCTTTAATTCCATTTATTATGGAAAAAACAGGTAAAACTGCT
    GAACAAGTATTGGAAATCTTAAACAAAGAATCAGGTTTATTAGGATTATCAGGTACATCT
    AGTGATTTACGTGACTTATCAGAAGAAGCTGAATCAGGTAAAGCACGTTCACAAATGGCT
    TTAGATGTATTTGCTTCTAAAATTCACAAATATATTGGATCATATGCTGCGAGAATGCAT
    GGTGTTGATGTTATCGTATTTACAGCAGGTATCGGTGAAAACTCAGTAGAAATTCGTGCG
    AAAGTTCTTGAAGGCTTAGAATTTATGGGTGTTTACTGGGATCCTAAGAAAAATGAAAAC
    TTACTACGTGGTAAAGAAGGATTTATTAACTATCCTCATTCACCAGTTAAAGTTGTCGTT
    ATTCCAACAGATGAAGAAAGTATGATTGCCCGTGATGTTATGACATTCGGTGGTTTAAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1203

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1605 [new locus tag: NWMN_RS09000 ]
  • symbol: AckA
  • description: acetate kinase
  • length: 400
  • theoretical pI: 5.88998
  • theoretical MW: 44042.3
  • GRAVY: -0.13125

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.1?  ExPASy
    Acetate kinase ATP + acetate = ADP + acetyl phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Fermentation acetate kinase (TIGR00016; EC 2.7.2.1; HMM-score: 561.8)
    and 1 more
    Metabolism Energy metabolism Fermentation butyrate kinase (TIGR02707; EC 2.7.2.7; HMM-score: 71.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Actin_ATPase (CL0108) Acetate_kinase; Acetokinase family (PF00871; HMM-score: 552.2)
    and 1 more
    no clan defined LacAB_rpiB; Ribose/Galactose Isomerase (PF02502; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003368
    • TAT(Tat/SPI): 0.000125
    • LIPO(Sec/SPII): 0.000381
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFTIEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESVAITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYLYSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAIDGGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGLSGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGENSVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARDVMTFGGLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_1580(gapB)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    NWMN_0961(pdhC)branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    NWMN_0962(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_0960(phdB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2149(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_2137(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_2120(rplM)50S ribosomal protein L13  [1] (data from MRSA252)
    NWMN_2133(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_2125(rplQ)50S ribosomal protein L17  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_2150(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    NWMN_1547(rpmA)50S ribosomal protein L27  [1] (data from MRSA252)
    NWMN_1166(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_2146(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_2119(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    NWMN_2127(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    NWMN_2148(rpsS)30S ribosomal protein S19  [1] (data from MRSA252)
    NWMN_1325(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_2086alkaline shock protein 23  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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