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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2086 [new locus tag: NWMN_RS12080 ]
- pan locus tag?: SAUPAN005578000
- symbol: NWMN_2086
- pan gene symbol?: asp23
- synonym:
- product: alkaline shock protein 23
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2086 [new locus tag: NWMN_RS12080 ]
- symbol: NWMN_2086
- product: alkaline shock protein 23
- replicon: chromosome
- strand: -
- coordinates: 2323828..2324337
- length: 510
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331278 NCBI
- RefSeq: YP_001333120 NCBI
- BioCyc:
- MicrobesOnline: 3707688 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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421
481ATGACTGTAGATAACAATAAAGCAAAACAAGCATACGACAATCAAACTGGTGTTAACGAA
AAAGAAAGAGAAGAGCGTCAAAAACAACAAGAACAAAATCAGGAGCCTCAATTCAAAAAC
AAATTAACATTCTCTGATGAAGTTGTTGAAAAAATTGCTGGTATCGCTGCACGTGAAGTT
AAAGGTATCTTAGATATGAAAGGTGGCTTAACTGATACATTCACTAATGCATTCTCAAGT
GGCAACAATGTTACTCAAGGTGTATCTGTTGAAGTTGGTGAAAAACAAGCTGCTGTAGAC
TTAAAAGTAATCTTAGAATATGGTGAATCAGCACCAAAAATCTTCCGTAAAGTAACTGAA
TTAGTTAAAGAACAAGTTAAATATATTACTGGTTTAGATGTAGTTGAAGTTAACATGCAA
GTTGACGATGTAATGACTCAAAAAGAGTGGAAACAAAAACACGAAAAAAATAACGAAAAC
AATAACCAAGAAAGACAAGGTTTACAATAA60
120
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510
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2086 [new locus tag: NWMN_RS12080 ]
- symbol: NWMN_2086
- description: alkaline shock protein 23
- length: 169
- theoretical pI: 4.8448
- theoretical MW: 19191.3
- GRAVY: -0.956805
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: no clan defined Asp23; Asp23 family, cell envelope-related function (PF03780; HMM-score: 117)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007466
- TAT(Tat/SPI): 0.00095
- LIPO(Sec/SPII): 0.001397
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTFSDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLKVILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNENNNQERQGLQ
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
NWMN_0167 (fadA) acetyl-CoA acetyltransferase-like protein [1] (data from MRSA252) NWMN_0168 (fadB) 3-hydroxyacyl-CoA dehydrogenase FadB-like protein [1] (data from MRSA252) NWMN_0961 (pdhC) branched-chain alpha-keto acid dehydrogenase subunit E2 [1] (data from MRSA252) NWMN_0962 (pdhD) dihydrolipoamide dehydrogenase [1] (data from MRSA252) NWMN_2152 (rplC) 50S ribosomal protein L3 [1] (data from MRSA252) NWMN_1151 (rplS) 50S ribosomal protein L19 [1] (data from MRSA252) NWMN_0510 (tufA) elongation factor Tu [1] (data from MRSA252) NWMN_0641 hypothetical protein [1] (data from MRSA252) NWMN_0811 hypothetical protein [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: SigB (activation) regulon
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p) - ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
J Bacteriol: 2004, 186(13);4085-99
[PubMed:15205410] [WorldCat.org] [DOI] (P p) - ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
J Bacteriol: 2011, 193(18);4954-62
[PubMed:21725011] [WorldCat.org] [DOI] (I p)