PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2205 [new locus tag: NWMN_RS12725 ]
• pan locus tag^?: SAUPAN005775000
• symbol: NWMN_2205
• pan gene symbol^?: —
• synonym:
• product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2205 [new locus tag: NWMN_RS12725 ]
• symbol: NWMN_2205
• product: D-isomer specific 2-hydroxyacid dehydrogenase family protein
• replicon: chromosome
• strand: +
• coordinates: 2425295..2426248
• length: 954
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331329 NCBI
• RefSeq: YP_001333239 NCBI
• BioCyc:
• MicrobesOnline: 3707807 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGAAAAAGTTTATGTTGCTGGTGCAATACCAGAAGTAGGTTTAAAACTTTTACAAGAA
  CATTTTGAAGTTGAAATGTATGAAGGTAAAGGATTAGTCGATAAAGACACTTTAATTAAA
  GGTGTTAAAAACGCGACTGCCTTAATTAGTTTATTATCTACAAACGTTGATAAAGATGTT
  ATCGATGCTGGTAAAGACTTAAAAATCATTGCCAACTATGGCGCTGGTTTTAATAATATT
  GATATCGAGTATGCCCGAGAAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACA
  AACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAA
  GGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGT
  AGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTA
  GCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTAATCGCAAAGAA
  GAAAAAGAACGAGAAATCGGTGCAAAATATGTAGATTTAGATACACTATTAAAGAATGCA
  GATTTTATCACTATCAACGCTGCTTATAATCCTAAAATGCATCATTTAATTGATACAGAA
  CAATTTAAAATGATGAAATCTACGGCGTATTTAATCAATGCCTCTCGTGGTCCAATCGTG
  CACGAACAAGCACTAGTGCAAGCATTGAAAGATAATGAAATTGAAGGTGCTGCACTTGAT
  GTATATGAATTTGAACCAGATATTACCGATGACTTAAAATCACTTAATAATGTAGTATTA
  ACACCGCATATTGGTAATGCTACATTTGAAGCACGTGACATGATGTCTAAAATTGTTGCT
  AATGCTGCAATTAGTGCCGTTCAAGGTGAGAAACCACAATTTGTCGTTAACTAA
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⊟Protein

⊟General

• locus tag: NWMN_2205 [new locus tag: NWMN_RS12725 ]
• symbol: NWMN_2205
• description: D-isomer specific 2-hydroxyacid dehydrogenase family protein
• length: 317
• theoretical pI: 4.91492
• theoretical MW: 34674.3
• GRAVY: -0.127445

⊟Function

• TIGRFAM:
  Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 268)
  and 2 more
  Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 17.5)
  Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 12.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 203)
  and 5 more
  Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 113.3)
  NADP_Rossmann (CL0063) IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 21)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 19.4)
  NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 18.3)
  AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001313
  • TAT(Tat/SPI): 0.000138
  • LIPO(Sec/SPII): 0.000235
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222417 NCBI
• RefSeq: YP_001333239 NCBI
• UniProt: A0A0H3KA24 UniProt

⊟Protein sequence

• MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAVARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications