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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0428 [new locus tag: NWMN_RS02420 ]
  • pan locus tag?: SAUPAN002178000
  • symbol: NWMN_0428
  • pan gene symbol?: metQ2
  • synonym:
  • product: ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0428 [new locus tag: NWMN_RS02420 ]
  • symbol: NWMN_0428
  • product: ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 480100..480942
  • length: 843
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAAAGATTGATTGGGTTAGTTATCGTAGCACTTGTATTATTAGCAGCGTGTGGTGGT
    AACAATGATAAAAAAGTAACAATTGGTGTCGCATCAAATGACACTAAGGCTTGGGAGAAG
    GTTAAAGAATTAGCTAAAAAAGATGATATTGATGTGGAGATTAAGCACTTCTCAGATTAC
    AATTTACCGAATAAAGCATTAAATGATGGTGATATTGATATGAATGCATTCCAACATTTT
    GCATTTTTAGATCAATATAAAAAGGCGCATAAAGGAACAAAGATTTCAGCATTAAGTACA
    ACAGTTTTAGCACCGTTGGGCATTTACTCAGATAAAATTAAAGATGTCAAAAAGGTTAAA
    GATGGTGCTAAAGTTGTCATTCCAAATGATGTGTCAAACCAAGCACGTGCACTTAAACTA
    TTAGAAGCAGCTGGTTTAATAAAACTGAAAAAAGATTTCGGATTAGCAGGCACGGTGAAA
    GATATAACGTCAAATCCAAAACATTTAAAAATTACTGCAGTAGATGCACAACAAACTGCA
    CGTGCTTTATCTGATGTCGATATTGCAGTTATTAATAACGGTGTAGCAACTAAAGCGGGT
    AAAGATCCTAAAAATGATCCGATATTTTTAGAAAAATCAAATTCAGATGCAGTAAAGCCA
    TATATTAATATTGTTGCAGTTAATGACAAAGACTTGGATAACAAAACATATGCTAAAATC
    GTAGAATTGTATCATTCAAAAGAAGCTCAAAAAGCGTTGCAGGAAGATGTCAAAGATGGA
    GAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    843

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0428 [new locus tag: NWMN_RS02420 ]
  • symbol: NWMN_0428
  • description: ABC transporter substrate-binding protein
  • length: 280
  • theoretical pI: 9.62721
  • theoretical MW: 30455.9
  • GRAVY: -0.315714

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Other lipoprotein, YaeC family (TIGR00363; HMM-score: 196.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PBP (CL0177) Lipoprotein_9; NLPA lipoprotein (PF03180; HMM-score: 288.4)
    and 1 more
    NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 16.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LLAACGG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
    • SP(Sec/SPI): 0.000502
    • TAT(Tat/SPI): 0.000079
    • LIPO(Sec/SPII): 0.999216
    • Cleavage Site: CS pos: 17-18. LAA-CG. Pr: 0.9998
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKELAKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTVLAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVKDITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAVKPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIETSLAK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of the methionine metabolism in Gram-positive bacteria: a variety of regulatory systems.
Nucleic Acids Res: 2004, 32(11);3340-53
[PubMed:15215334] [WorldCat.org] [DOI] (I e)Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)

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