PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0427 [new locus tag: NWMN_RS02415 ]
• pan locus tag^?: SAUPAN002177000
• symbol: NWMN_0427
• pan gene symbol^?: metP2
• synonym:
• product: ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0427 [new locus tag: NWMN_RS02415 ]
• symbol: NWMN_0427
• product: ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 479404..480063
• length: 660
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330229 NCBI
• RefSeq: YP_001331461 NCBI
• BioCyc:
• MicrobesOnline: 3705958 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTTTGGTTCTGATTTAGACAGTGCACAGTTATTACAAGCATTGTACGAAACGCTATAT
  ATGGTATCTATTGCTTTATTTTTAGGAGCAGTGATTGGTATTCCATTAGGTGTCTTATTG
  GTAATTACTCGAAAACAAGGCATATGGCCCAATATAGTGATACATCAAGTTTTAAATCCT
  TTAATCAATATTTTAAGGTCACTACCATTTATTATTTTGTTAATTGCGATTGTGCCATTC
  ACAAAATTAGTAGTAGGTACTTCAATTGGTACGACTGCTGCCATCGTGCCTTTAACAGTA
  TATGTGGCACCTTACATTGCAAGACTTGTTGAAAACTCATTATTGGAAGTAGACGAGGGG
  ATTATTGAAGCGGCGAAAGCGATGGGCGCTTCACCACTACAAATCATTAGATATTTTTTA
  ATTCCTGAAGCTTTAGGTTCGTTAGTATTAGCAATTACCACTGCGATTATTGGACTTATT
  GGAAGTACGGCGATGGCAGGAGCTGTTGGCGGTGGTGGTATAGGAGACTTAGCTTTAGTG
  TATGGTTATCAAAGATTTGATACGACGGTCATTATTATTACCGTTATTGTATTAGTCATT
  ATTGTCCAAGTGATTCAAACGCTAGGGAATGTTCTAGCTAGATTCATACGTAGACATTAA
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⊟Protein

⊟General

• locus tag: NWMN_0427 [new locus tag: NWMN_RS02415 ]
• symbol: NWMN_0427
• description: ABC transporter permease
• length: 219
• theoretical pI: 7.66251
• theoretical MW: 23309.9
• GRAVY: 1.15479

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 73.4)
  and 11 more
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 38.3)
  ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 33.2)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 31.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 22.7)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 21.9)
  ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 20.3)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 19)
  2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 18.4)
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.2)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 16.6)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 14.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 71.6)
  and 1 more
  FtsX; FtsX-like permease family (PF02687; HMM-score: 7.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001296
  • TAT(Tat/SPI): 0.001172
  • LIPO(Sec/SPII): 0.002057
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 151220639 NCBI
• RefSeq: YP_001331461 NCBI
• UniProt: A0A0H3K6C4 UniProt

⊟Protein sequence

• MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITRKQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYVAPYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLIGSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRRH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0426 > NWMN_0427 > NWMN_0428

⊟Regulation

• regulator: CymR* (repression) regulon

  CymR*

  (TF)

  important in Cysteine metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications