PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1957 [new locus tag: SAUSA300_RS10735 ]
• pan locus tag^?: SAUPAN001394000
• symbol: SAUSA300_1957
• pan gene symbol^?: dnaD2
• synonym:
• product: phiPVL ORF046-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1957 [new locus tag: SAUSA300_RS10735 ]
• symbol: SAUSA300_1957
• product: phiPVL ORF046-like protein
• replicon: chromosome
• strand: -
• coordinates: 2114638..2115531
• length: 894
• essential: unknown

⊟Accession numbers

• Gene ID: 3914952 NCBI
• RefSeq: YP_494608 NCBI
• BioCyc: see SAUSA300_RS10735
• MicrobesOnline: 1293472 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACTGGTTGGATAAGTATTGATCGCTCAATTCAAAATCATTGGCTATTTAAAGAAAAG
  AGAACATTTTCAAAGTTTGAAGCATGGATATATTTACTCATGGAAGCGAATCATTCAAAG
  GCAAAAGTGCCTATAGGAAACCAAATTGTAACCGTTGAAAGAGGACAAAGATTAACATCT
  ATTTTGACCTTGTCTGACCTTTTTAACTGGTCACGATTTAAAGTGAAAACCTTCCTTGAC
  TTACTCGAGAGTGATGGAATGTTAGAAGTCAAAACAACATCAAAATATACCCTTATAACC
  ATTGTCAATTATGACTTTTATCAAAGTGAGCAGGGCAGGAACCAACATCAAAACGACATC
  AAACCAACATCAAAACAACATCAGTCAAACATCAACCCAACATCAAAACAACATCAAACC
  AACACAAACAATAATGATAATAAAGATAATAATGAAAAGAATGTGAATAATGAGAAGAAG
  AAGACAACCGCCTTCGACTTCTTCCAAGATAACGGATTCGGTTTCATAACTTCTTACAAT
  TTAGACGATTTAAATTATTATCTTGATTCATTTGAAAATGATTCAGATGAAATAGTTACC
  GCATCACTTAAAATCGCTAAAGACAGAAACAAAGTTACTTGGGGATATGCTAAAAGCATT
  TTGAATACATGGCTTAATGCAAACTTGAAATCTATTGAACAAGTACGTGCATTTGAAAAG
  CAACAACTTGAAAGCAAAAAACAAAATTATAAACCTTTCGTTAAACAATCAAAAGAAAAA
  ACACCCAAATGGCTCACAGACAGCACGAGAGAAACGAAAACGCCGGAAGTAGATGAAAAC
  CTTGAGAAAGACAGAGAAGCTTTTATTAAGCGTCTAAATAGCAAATGGGAGTGA
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⊟Protein

⊟General

• locus tag: SAUSA300_1957 [new locus tag: SAUSA300_RS10735 ]
• symbol: SAUSA300_1957
• description: phiPVL ORF046-like protein
• length: 297
• theoretical pI: 9.58803
• theoretical MW: 34981.9
• GRAVY: -0.967677

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 79.1)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 79.1)
• TheSEED  :
  • Phage DNA replication protein O
• PFAM:
  no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 47.6)
  and 3 more
  HTH (CL0123) Phage_rep_org_N; N-terminal phage replisome organiser (Phage_rep_org_N) (PF09681; HMM-score: 14)
  no clan defined UPF0561; Uncharacterised protein family UPF0561 (PF10573; HMM-score: 11.8)
  CPSF100_C; Cleavage and polyadenylation factor 2 C-terminal (PF13299; HMM-score: 10.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007995
  • TAT(Tat/SPI): 0.000465
  • LIPO(Sec/SPII): 0.000664
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159906 NCBI
• RefSeq: YP_494608 NCBI
• UniProt: A0A0H2XDV9 UniProt

⊟Protein sequence

• MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVPIGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIVNYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKKKTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAKSILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPEVDENLEKDREAFIKRLNSKWE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: dut < SAUSA300_1950 < SAUSA300_1951 < SAUSA300_1952 < SAUSA300_1953 < SAUSA300_1954 < SAUSA300_1955 < SAUSA300_1956 < SAUSA300_1957 < SAUSA300_1958 < SAUSA300_1959

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications