PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1959 [new locus tag: SAUSA300_RS10745 ]
• pan locus tag^?: SAUPAN001396000
• symbol: SAUSA300_1959
• pan gene symbol^?: —
• synonym:
• product: phiPVL ORF044-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1959 [new locus tag: SAUSA300_RS10745 ]
• symbol: SAUSA300_1959
• product: phiPVL ORF044-like protein
• replicon: chromosome
• strand: -
• coordinates: 2116032..2116517
• length: 486
• essential: unknown

⊟Accession numbers

• Gene ID: 3914443 NCBI
• RefSeq: YP_494610 NCBI
• BioCyc: see SAUSA300_RS10745
• MicrobesOnline: 1293474 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACTGCTGGAACACAACAAGCTATGAATTTTGAAAGTCATCGCTTATGCACGATTAAG
  GCAAAGCAAGAGCTGCGAATAGGTACATGGTCAATTCTACCGTTTGACATCGAACATGAT
  GCTAACGAGCCTGTGGCTTTCTTATTACAAAGCACACTAGGTTATAAGGTTCTGTATGTT
  ACTGATACAAAGTATTTGAAATACAAATTTAATGGCATTACGCACATGATGCTAGAAGTT
  AATTATATCTATGAACAAATGCAAGAAAACATAAAAAACGGCAGTGTGCACAGCACATTA
  GCAAACAGAATTATGGAGTCTCATTTTAGCTTAGAACATGCTATCGGAATGTTAAAAGCA
  AATGATTTAACTAGACTCGAAGAAATACATTTAATTCATTTAAGTAGTCAAAATTCAAAT
  GCAAAATACATTAAAAGTGAAATACAAAAAGTGACGGGCGCGCCCGTTTATTTTGGAGGT
  TTATAA
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  486
  

⊟Protein

⊟General

• locus tag: SAUSA300_1959 [new locus tag: SAUSA300_RS10745 ]
• symbol: SAUSA300_1959
• description: phiPVL ORF044-like protein
• length: 161
• theoretical pI: 7.29216
• theoretical MW: 18396
• GRAVY: -0.239752

⊟Function

• TIGRFAM:
• TheSEED  :
  • Metallo-beta-lactamase superfamily domain protein in prophage
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Metallo-beta-lactamase superfamily domain protein in prophage
• PFAM:
  Metallo-HOrase (CL0381) Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 20.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004348
  • TAT(Tat/SPI): 0.000191
  • LIPO(Sec/SPII): 0.001197
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161678 NCBI
• RefSeq: YP_494610 NCBI
• UniProt: A0A0H2XHS6 UniProt

⊟Protein sequence

• MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVLYVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIGMLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: dut < SAUSA300_1950 < SAUSA300_1951 < SAUSA300_1952 < SAUSA300_1953 < SAUSA300_1954 < SAUSA300_1955 < SAUSA300_1956 < SAUSA300_1957 < SAUSA300_1958 < SAUSA300_1959

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications