PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1952 [new locus tag: SAUSA300_RS10710 ]
• pan locus tag^?: SAUPAN001386000
• symbol: SAUSA300_1952
• pan gene symbol^?: —
• synonym:
• product: phiPV083 ORF027-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1952 [new locus tag: SAUSA300_RS10710 ]
• symbol: SAUSA300_1952
• product: phiPV083 ORF027-like protein
• replicon: chromosome
• strand: -
• coordinates: 2112993..2113364
• length: 372
• essential: unknown

⊟Accession numbers

• Gene ID: 3914140 NCBI
• RefSeq: YP_494603 NCBI
• BioCyc: see SAUSA300_RS10710
• MicrobesOnline: 1293467 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAGTGAACTATGAAACAGGATTCCAAATAGGCGTAATGGAAGCTAGGTTGAAGAAG
  ATGAGAAAACAACGTGATGAGTACAAGAAGCAACGTGACGAGCTTATTGGGGATATAGCT
  AAGTTAAGAGAGCGTAACGAAGAGCTGGAGAACATGTGGCGCACGCTTAAAAATGAATTG
  TTTGGAAGATACGAATTTTACCGTTTTAGACTTAGCGAACTACAGATTGAGAGCAGAGCG
  AACAAGGAAGTAGCTATATATAGAAGAGCTGAAATCAACTTAAGTGTTATATTGTGCCGA
  ATGGACAAACTAGACGGAACAAATGAGTTCTACGAATTTTTAGGGCAAATGGAGGATGAC
  ACAAATGAATAA
  60
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  372
  

⊟Protein

⊟General

• locus tag: SAUSA300_1952 [new locus tag: SAUSA300_RS10710 ]
• symbol: SAUSA300_1952
• description: phiPV083 ORF027-like protein
• length: 123
• theoretical pI: 5.43795
• theoretical MW: 15018
• GRAVY: -0.956911

⊟Function

• TIGRFAM:
• TheSEED  :
  • Hypothetical protein, SAB1734c homolog [SA bacteriophages 11, Mu50B]
• PFAM:
  no clan defined Myosin_tail_1; Myosin tail (PF01576; HMM-score: 18.1)
  Cep57_CLD_2; Centrosome localisation domain of PPC89 (PF14197; HMM-score: 15.9)
  Phage_GP20; Phage minor structural protein GP20 (PF06810; HMM-score: 15.5)
  TPR_MLP1_2; TPR/MLP1/MLP2-like protein (PF07926; HMM-score: 14.6)
  and 4 more
  TMF_TATA_bd; TATA element modulatory factor 1 TATA binding (PF12325; HMM-score: 14)
  NYD-SP28_assoc; Sperm tail C-terminal domain (PF14775; HMM-score: 13.8)
  Cortex-I_coil; Cortexillin I, coiled coil (PF09304; HMM-score: 13)
  FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 11)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006334
  • TAT(Tat/SPI): 0.000998
  • LIPO(Sec/SPII): 0.002299
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160868 NCBI
• RefSeq: YP_494603 NCBI
• UniProt: A0A0H2XHF7 UniProt

⊟Protein sequence

• MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRERNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMDKLDGTNEFYEFLGQMEDDTNE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: dut < SAUSA300_1950 < SAUSA300_1951 < SAUSA300_1952 < SAUSA300_1953 < SAUSA300_1954 < SAUSA300_1955 < SAUSA300_1956 < SAUSA300_1957 < SAUSA300_1958 < SAUSA300_1959

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications