PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1958 [new locus tag: SAUSA300_RS10740 ]
• pan locus tag^?: SAUPAN001395000
• symbol: SAUSA300_1958
• pan gene symbol^?: ssb2
• synonym:
• product: single-strand binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1958 [new locus tag: SAUSA300_RS10740 ]
• symbol: SAUSA300_1958
• product: single-strand binding protein
• replicon: chromosome
• strand: -
• coordinates: 2115561..2116031
• length: 471
• essential: unknown

⊟Accession numbers

• Gene ID: 3914250 NCBI
• RefSeq: YP_494609 NCBI
• BioCyc: see SAUSA300_RS10740
• MicrobesOnline: 1293473 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  
  ATGCTAAACAGAACAATATTAGTTGGTCGTTTAACTAGAGACCCAGAATTAAGAACCACT
  CAAAATGGTGTAAATGTAGCATCATTCACATTAGCAGTTAACCGTACATTTACAAATGCA
  CAAGGCGAGCGCGAGGCAGACTTTATTAATATCATCGTATTTAAAAAACAAGCAGAGAAC
  GTTAATAAATACCTATCTAAAGGATCGTTGGCGGGCGTAGATGGTAGGTTACAAACGCGG
  AACTATGAAAATAAGGAAGGTCAACGTGTATACGTTACGGAAGTTATTGCTGATAGTATT
  CAATTTTTAGAACCGAAAAACTCAAATGACACTCAACAAGATTTATATCAACAACAAGTA
  CAACAAACACGTGGACAATCGCAATATTCAAATAACAAACCAGTAAAAGATAATCCGTTT
  GCGAATGCAAATGGTCCGATTGAACTAAATGATGATGATTTACCATTCTAA
  60
  120
  180
  240
  300
  360
  420
  471
  

⊟Protein

⊟General

• locus tag: SAUSA300_1958 [new locus tag: SAUSA300_RS10740 ]
• symbol: SAUSA300_1958
• description: single-strand binding protein
• length: 156
• theoretical pI: 5.26974
• theoretical MW: 17668.4
• GRAVY: -0.807692

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair single-stranded DNA-binding protein (TIGR00621; HMM-score: 153.5)
  and 1 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal replication protein PriB (TIGR04418; HMM-score: 26.4)
• TheSEED  :
  • Single-stranded DNA-binding protein
  DNA Metabolism DNA repair DNA repair, bacterial  Single-stranded DNA-binding protein
  and 1 more
  DNA Metabolism DNA repair DNA repair, bacterial RecFOR pathway  Single-stranded DNA-binding protein
• PFAM:
  OB (CL0021) SSB; Single-strand binding protein family (PF00436; HMM-score: 127.1)
  and 1 more
  tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 18.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014241
  • TAT(Tat/SPI): 0.002993
  • LIPO(Sec/SPII): 0.002245
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162071 NCBI
• RefSeq: YP_494609 NCBI
• UniProt: A0A0H2XJY5 UniProt

⊟Protein sequence

• MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGEREADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQFLEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: dut < SAUSA300_1950 < SAUSA300_1951 < SAUSA300_1952 < SAUSA300_1953 < SAUSA300_1954 < SAUSA300_1955 < SAUSA300_1956 < SAUSA300_1957 < SAUSA300_1958 < SAUSA300_1959

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications