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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1949 [new locus tag: SAUSA300_RS10695 ]
  • pan locus tag?: SAUPAN001416000
  • symbol: dut
  • pan gene symbol?: dut
  • synonym:
  • product: dUTP diphosphatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1949 [new locus tag: SAUSA300_RS10695 ]
  • symbol: dut
  • product: dUTP diphosphatase
  • replicon: chromosome
  • strand: -
  • coordinates: 2112006..2112539
  • length: 534
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGACTAACATCCTACAAGTAAAGCTATTATCAGAAAACGCTAGAATGCCAGAACGAAAT
    CATAAGACGGATGCAGGTTATGACATATTCTCAGCTGAAACTGTCGTACTTGAGCCACAA
    GAAAAGGCAGTGATCAAAACAGATGTAGCTGTAAGCATACCAGAGGGCTATGTCGGACTA
    TTAACTAGCCGTAGTGGTGTAAGTAGTAAAACGCATTTAGTGATTGAAACAGGCAAGATA
    GACGCGGGATATCACGGCAATTTAGGGATTAATATCAAGAATGATAATGAAACGTTAGAG
    AGTGAGGATATGAGTAACTTTGGTCGGAGTCCTGCTGGGATAGATGGAAAGTATGCCCGA
    CTACCTGTAACAGATAAAATTTTATGTATGAATGGTAGTTATGTCATAAACAAAGGCGAC
    AAACTAGCTCAATTGGTTATTGTGCCTATATGGACACCTGAACTAAAGCAAGTGGAGGAA
    TTCGAGAGTGTTTCAGAACGTGGAGCAAAAGGCTTCGGAAGTAGCGGAGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    534

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1949 [new locus tag: SAUSA300_RS10695 ]
  • symbol: Dut
  • description: dUTP diphosphatase
  • length: 177
  • theoretical pI: 5.05828
  • theoretical MW: 19196.6
  • GRAVY: -0.262712

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.1.23?  ExPASy
    dUTP diphosphatase dUTP + H2O = dUMP + diphosphate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism dUTP diphosphatase (TIGR00576; EC 3.6.1.23; HMM-score: 114.9)
    and 1 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism deoxycytidine triphosphate deaminase (TIGR02274; EC 3.5.4.13; HMM-score: 34.6)
  • TheSEED  :
    • Phage deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23)
  • PFAM:
    dUTPase (CL0153) dUTPase; dUTPase (PF00692; HMM-score: 91.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004744
    • TAT(Tat/SPI): 0.000356
    • LIPO(Sec/SPII): 0.000477
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAVIKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESEDMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEEFESVSERGAKGFGSSGV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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