PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1954 [new locus tag: SAUSA300_RS10720 ]
• pan locus tag^?: SAUPAN001391000
• symbol: SAUSA300_1954
• pan gene symbol^?: —
• synonym:
• product: phiPVL ORF050-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1954 [new locus tag: SAUSA300_RS10720 ]
• symbol: SAUSA300_1954
• product: phiPVL ORF050-like protein
• replicon: chromosome
• strand: -
• coordinates: 2113619..2113996
• length: 378
• essential: unknown

⊟Accession numbers

• Gene ID: 3914131 NCBI
• RefSeq: YP_494605 NCBI
• BioCyc: see SAUSA300_RS10720
• MicrobesOnline: 1293469 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  GTGGATAAAATGGCGAGAAAAGCAAGGATTGTAACAATAAATGATAAACCATATAGGTTT
  ACCAAATCTGAAATGGAATTAATAGAAAGTCACGGTATAACCGCTGGAATGGTTTCTAAG
  AGAGTAAAAGACGGTTGGGAACTACATGAAGCAATGGACGCACCAGAAGGTACGCGTTTA
  AGCGAGTACAGAGAAAAGAAAACAATAGAAAGACTGGAACAAGCTAGACTCGAACGCAAA
  TTGGAAAGAAAGCGAAAGAAAGAGGCTGAGCTAAGAAGAAAGAAGCCACATTTGTTTAAT
  GTACCACAGAAACATCCAAGAGGACGTTATGCGTGCTACCTGATGGAAAACGACATATTC
  GTGAAAGTTAAGAAGTAG
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  378
  

⊟Protein

⊟General

• locus tag: SAUSA300_1954 [new locus tag: SAUSA300_RS10720 ]
• symbol: SAUSA300_1954
• description: phiPVL ORF050-like protein
• length: 125
• theoretical pI: 10.6685
• theoretical MW: 14983.4
• GRAVY: -1.1016

⊟Function

• TIGRFAM:
• TheSEED  :
  • Phage phi 11 orf21 protein homolog
• PFAM:
  no clan defined PVL_ORF50; PVL ORF-50-like family (PF07768; HMM-score: 149.3)
  and 2 more
  TMEM247; Transmembrane protein 247 (PF15444; HMM-score: 12.7)
  YqzE; YqzE-like protein (PF14038; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004221
  • TAT(Tat/SPI): 0.000405
  • LIPO(Sec/SPII): 0.000523
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160860 NCBI
• RefSeq: YP_494605 NCBI
• UniProt: A0A0H2XFG8 UniProt

⊟Protein sequence

• MDKMARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWELHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKHPRGRYACYLMENDIFVKVKK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: dut < SAUSA300_1950 < SAUSA300_1951 < SAUSA300_1952 < SAUSA300_1953 < SAUSA300_1954 < SAUSA300_1955 < SAUSA300_1956 < SAUSA300_1957 < SAUSA300_1958 < SAUSA300_1959

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications