PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1908 [new locus tag: NWMN_RS11000 ]
• pan locus tag^?: SAUPAN001394000
• symbol: NWMN_1908
• pan gene symbol^?: dnaD2
• synonym:
• product: phage regulatory protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1908 [new locus tag: NWMN_RS11000 ]
• symbol: NWMN_1908
• product: phage regulatory protein
• replicon: chromosome
• strand: -
• coordinates: 2118960..2119844
• length: 885
• essential: unknown

⊟Accession numbers

• Gene ID: 5331172 NCBI
• RefSeq: YP_001332942 NCBI
• BioCyc:
• MicrobesOnline: 3707496 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACTGGTTGGATAAAACTTCATAGAAAACTATTAGATTCGCCTATTTTTCAGAACGAA
  AAGTTATTCAAAGTATTTGCATATTGTCTTATGAAGGCTAGTCATAAGGATCATACACAG
  CTTGTTGGCAGACGAGTTGTTGAATTAGAAAAAGGTCAATTTGTGTTCGGGAGAAAGCGA
  GCAAGCGAAGAGTTACGTCTCAAAGAATCCACAGTAAGAGACTACATAAAGCTTTTAGAA
  AACCTTGGAACTATCGTCGTAAAGTCCGACAACAAATTTTCTGTTATAACCGTTGTCAAT
  TGGGCGATTTATCAAAGTATGGAAGAAAATTCCGACAGCAAAAACGACAACAAATCAACA
  ACAAATCAACAACAAATGGACAACAAATGGACAACAAATCAACAACAAATCAACACAAAC
  AAGAATGTAAAGAATGGGGATAATGTAAAGAATGGTGAGAATGAGAAGAAGAAGGTAACC
  GCCTTCGACTTCTTCCAAGATAACGGATTCGGTTTCATAACTCCTTACAATTTAGACGAT
  TTAAATTATTATCTTGATTCATTTGAAAATGATTCAGATCAAATAGTTACCGCATCACTT
  AAAATCGCTAAAGACAGAAATAAAGTTACTTGGGGATATGCTAAAAGCATTTTGAATACA
  TGGCTTAATGCAAACTTGAAATCTATTGAACAAGTACGTGCATTTGAAAAGCAACAACTT
  GAAAGCAAAAAACAAAATTATAAACCTTTCGTTAAACAATCAAAAGAAAAAACACCCAAA
  TGGCTCACAGACAGCACGAGAGAAACGAAAACGCCGGAAGTAGATGAAAACCTTGAGAAA
  GACAGAGAAGCTTTTATTAAGCGTCTAAATAGCAAATGGGAGTGA
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⊟Protein

⊟General

• locus tag: NWMN_1908 [new locus tag: NWMN_RS11000 ]
• symbol: NWMN_1908
• description: phage regulatory protein
• length: 294
• theoretical pI: 9.87814
• theoretical MW: 34490.7
• GRAVY: -0.928912

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 79.3)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 79.3)
• TheSEED: data available for USA300_FPR3757
• PFAM:
  no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 48.9)
  and 4 more
  HTH (CL0123) Phage_rep_org_N; N-terminal phage replisome organiser (Phage_rep_org_N) (PF09681; HMM-score: 17.4)
  Met_repress (CL0057) DndE; DNA sulphur modification protein DndE (PF08870; HMM-score: 15.3)
  HTH (CL0123) HTH_29; Winged helix-turn helix (PF13551; HMM-score: 12.5)
  no clan defined Ribosomal_S17_N; Ribosomal_S17 N-terminal (PF16205; HMM-score: 8.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002388
  • TAT(Tat/SPI): 0.00029
  • LIPO(Sec/SPII): 0.000595
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222120 NCBI
• RefSeq: YP_001332942 NCBI
• UniProt: A0A0H3KHR3 UniProt

⊟Protein sequence

• MTGWIKLHRKLLDSPIFQNEKLFKVFAYCLMKASHKDHTQLVGRRVVELEKGQFVFGRKRASEELRLKESTVRDYIKLLENLGTIVVKSDNKFSVITVVNWAIYQSMEENSDSKNDNKSTTNQQQMDNKWTTNQQQINTNKNVKNGDNVKNGENEKKKVTAFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDQIVTASLKIAKDRNKVTWGYAKSILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPEVDENLEKDREAFIKRLNSKWE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1908 < NWMN_1909 < NWMN_1910

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications