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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000946
  • pan locus tag?: SAUPAN003133000
  • symbol: opp3b
  • pan gene symbol?: opp3B
  • synonym:
  • product: oligopeptide ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000946
  • symbol: opp3b
  • product: oligopeptide ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 949738..950664
  • length: 927
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGGAAATATATTTTCAAACGATTTATTTATATGCTTATTTCTTTATTTATTATTATT
    ACAATTACATTTTTCTTAATGAAATTAATGCCAGGTTCGCCATTTAACGATGCTAAATTA
    AATGCTGAACAAAAAGAAATTTTAAATGAAAAATATGGATTAAATGATCCTGTAGCTACG
    CAGTATTTACATTATTTAAAAAATGTTGTTACAGGCGATTTTGGTAATTCATTCCAGTAT
    CATAATCAACCTGTGTGGGATTTGATTAAACCGAGACTACTACCTTCTTTTGAAATGGGT
    CTTACAGCAATGTTCATCGGTGTGATACTGGGACTTATTTTAGGTGTTGCAGCAGCTACT
    AAACAAAATTCTTGGGTTGACTATACAACTACAGTTATTTCAGTTATTGCAGTATCTGTA
    CCATCTTTTGTACTTGCTGTACTTTTACAATATGTATTTGCAGTTAAATTAAGATGGTTC
    CCAGTAGCTGGATGGGAAGGTTTTTCGACCGCGGTATTACCGTCACTTGCATTATCTGCA
    GCTGTTTTAGCAACTGTCGCCAGATACATAAGAGCAGAGATGATAGAGGTATTAAGTTCA
    GACTATATTTTATTAGCGAGAGCTAAAGGTAATTCGACAATGCGTGTACTTTTTGGACAT
    GCACTTAGAAATGCTTTAATTCCAATTATTACAATTATCGTTCCCATGTTAGCAAGTATT
    TTAACAGGCACTTTAACAATTGAAAATATTTTTGGGGTTCCTGGATTAGGGGATCAATTC
    GTACGTTCAATTACAACAAATGATTTCTCAGTAATCATGGCAATCACACTATTATTTAGC
    ACACTGTTTATCGTTTCTATTTTTATTGTAGATATTTTGTACGGTGTGATAGATCCACGA
    ATTCGTGTTCAAGGAGGTAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000946
  • symbol: Opp3b
  • description: oligopeptide ABC transporter permease
  • length: 308
  • theoretical pI: 9.822
  • theoretical MW: 34142.4
  • GRAVY: 0.719156

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 228.4)
    and 2 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 19.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 15.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 130)
    and 3 more
    no clan defined BPD_transp_1_N; Binding-prot-dependent transport system membrane comp, N-term (PF19300; HMM-score: 38.1)
    NPCC; Nuclear pore complex component (PF08058; HMM-score: 10.3)
    Nup120_hel_sf (CL0833) NUP120_helical_2; Nucleoporin NUP120, helical domain (PF22114; HMM-score: 9.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.294557
    • TAT(Tat/SPI): 0.003898
    • LIPO(Sec/SPII): 0.007787
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQKEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLTAMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWFPVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLFGHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAITLLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J Bacteriol: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)

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