PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0887 [new locus tag: SAUSA300_RS04785 ]
• pan locus tag^?: SAUPAN003133000
• symbol: oppB
• pan gene symbol^?: opp3B
• synonym:
• product: oligopeptide ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0887 [new locus tag: SAUSA300_RS04785 ]
• symbol: oppB
• product: oligopeptide ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 974149..975075
• length: 927
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914794 NCBI
• RefSeq: YP_493587 NCBI
• BioCyc: see SAUSA300_RS04785
• MicrobesOnline: 1292402 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  901
  
  ATGGGGAAATATATTTTCAAACGATTTATTTATATGCTTATTTCTTTATTTATTATTATT
  ACAATTACATTTTTCTTAATGAAATTAATGCCAGGTTCGCCATTTAACGATGCTAAATTA
  AATGCTGAACAAAAAGAAATTTTAAATGAAAAATATGGATTAAATGATCCTGTAGCTACG
  CAGTATTTACATTATTTAAAAAATGTTGTTACAGGCGATTTTGGTAATTCATTCCAGTAT
  CATAATCAACCTGTGTGGGATTTGATTAAACCGAGACTACTACCTTCTTTTGAAATGGGT
  CTTACAGCAATGTTCATCGGTGTGATACTGGGACTTATTTTAGGTGTTGCAGCAGCTACT
  AAACAAAATTCTTGGGTTGACTATACAACTACAGTTATTTCAGTTATTGCAGTATCTGTA
  CCATCTTTTGTACTTGCTGTACTTTTACAATATGTATTTGCAGTTAAATTAAGATGGTTC
  CCAGTAGCTGGATGGGAAGGTTTTTCGACCGCGGTATTACCGTCACTTGCATTATCTGCA
  GCTGTTTTAGCAACTGTCGCCAGATACATAAGAGCAGAGATGATAGAGGTATTAAGTTCA
  GACTATATTTTATTAGCGAGAGCTAAAGGTAATTCGACAATGCGTGTACTTTTTGGACAT
  GCACTTAGAAATGCTTTAATTCCAATTATTACAATTATCGTTCCCATGTTAGCAAGTATT
  TTAACAGGCACTTTAACAATTGAAAATATTTTTGGAGTTCCTGGATTAGGGGATCAATTC
  GTACGTTCAATTACAACAAATGATTTCTCAGTAATCATGGCAATCACACTATTATTTAGC
  ACACTGTTTATCGTTTCTATTTTTATTGTAGATATTTTGTACGGTGTGATAGATCCACGA
  ATTCGTGTTCAAGGAGGTAAAAAATAA
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  927
  

⊟Protein

⊟General

• locus tag: SAUSA300_0887 [new locus tag: SAUSA300_RS04785 ]
• symbol: OppB
• description: oligopeptide ABC transporter permease
• length: 308
• theoretical pI: 9.822
• theoretical MW: 34142.4
• GRAVY: 0.719156

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 228.4)
  and 2 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 19.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 15.3)
• TheSEED  :
  • Oligopeptide ABC transporter (EC 7.4.2.6), permease protein OppB
  Membrane Transport ABC transporters ABC transporter oligopeptide (TC 3.A.1.5.1)  Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 126.6)
  and 2 more
  no clan defined NPCC; Nuclear pore complex component (PF08058; HMM-score: 11.3)
  Renin_r; Renin receptor-like protein (PF07850; HMM-score: 9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.294557
  • TAT(Tat/SPI): 0.003898
  • LIPO(Sec/SPII): 0.007787
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 87161240 NCBI
• RefSeq: YP_493587 NCBI
• UniProt: A0A0H2XID7 UniProt

⊟Protein sequence

• MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQKEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLTAMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWFPVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLFGHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAITLLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: oppB > oppC > oppD > oppF > oppA

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J Bacteriol: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)