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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0991 [new locus tag: SACOL_RS05065 ]
  • pan locus tag?: SAUPAN003133000
  • symbol: oppB
  • pan gene symbol?: opp3B
  • synonym:
  • product: oligopeptide ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0991 [new locus tag: SACOL_RS05065 ]
  • symbol: oppB
  • product: oligopeptide ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 997700..998548
  • length: 849
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAATTAATGCCAGGTTCGCCATTTAACGATGCTAAATTAAATGCTGAACAAAAAGAA
    ATTTTAAATGAAAAATATGGATTAAATGATCCTGTAGCTACGCAGTATTTACATTATTTA
    AAAAATGTTGTTACAGGCGATTTTGGTAATTCATTCCAGTATCATAATCAACCTGTGTGG
    GATTTGATTAAACCGAGACTACTACCTTCTTTTGAAATGGGTCTTACAGCAATGTTCATC
    GGTGTGATACTGGGACTTATTTTAGGTGTTGCAGCAGCTACTAAACAAAATTCTTGGGTT
    GACTATACAACTACAGTTATTTCAGTTATTGCAGTATCTGTACCATCTTTTGTACTTGCT
    GTACTTTTACAATATGTATTTGCAGTTAAATTAAGATGGTTCCCAGTAGCTGGATGGGAA
    GGTTTTTCGACCGCGGTATTACCGTCACTTGCATTATCTGCAGCTGTTTTAGCAACTGTC
    GCCAGATACATAAGAGCAGAGATGATAGAGGTATTAAGTTCAGACTATATTTTATTAGCG
    AGAGCTAAAGGTAATTCGACAATGCGTGTACTTTTTGGACATGCACTTAGAAATGCTTTA
    ATTCCAATTATTACAATTATCGTTCCCATGTTAGCAAGTATTTTAACAGGCACTTTAACA
    ATTGAAAATATTTTTGGAGTTCCTGGATTAGGGGATCAATTCGTACGTTCAATTACAACA
    AATGATTTCTCAGTAATCATGGCAATCACACTATTATTTAGCACACTGTTTATCGTTTCT
    ATTTTTATTGTAGATATTTTGTACGGTGTGATAGATCCACGAATTCGTGTTCAAGGAGGT
    AAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    849

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0991 [new locus tag: SACOL_RS05065 ]
  • symbol: OppB
  • description: oligopeptide ABC transporter permease
  • length: 282
  • theoretical pI: 9.45037
  • theoretical MW: 30927.3
  • GRAVY: 0.63227

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 218)
    and 2 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 20.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 15.5)
  • TheSEED  :
    • Oligopeptide ABC transporter (EC 7.4.2.6), permease protein OppB
    Membrane Transport ABC transporters ABC transporter oligopeptide (TC 3.A.1.5.1)  Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 127)
    and 1 more
    no clan defined Renin_r; Renin receptor-like protein (PF07850; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012872
    • TAT(Tat/SPI): 0.00052
    • LIPO(Sec/SPII): 0.001919
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLMPGSPFNDAKLNAEQKEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLTAMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWFPVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLFGHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAITLLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J Bacteriol: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)

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