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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000950
  • pan locus tag?: SAUPAN003137000
  • symbol: JSNZ_000950
  • pan gene symbol?: opp3A
  • synonym:
  • product: peptide ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000950
  • symbol: JSNZ_000950
  • product: peptide ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 953782..955437
  • length: 1656
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACGAGAAAATTTAGAACACTTATTTTAATTTTGATTGCTACAATTGCATTAAGTGGT
    TGTGCTAATGATGATGGTATTTATTCAGATAAAGGTCAAGTATTCAGAAAAATTTTGTCA
    TCAGACTTAACATCCCTTGATACATCATTAATAACGGATGAAATATCTTCTGAAGTGACT
    GCGCAAACATTCGAAGGTTTATACACATTAGGAAAAGGTGACAAACCGGTGTTAGGTGTT
    GCGAAAGCTTTTCCTGAAAAGAGTAAAGGTGGTAAAACTTTAAAGGTTAAATTAAGAAGC
    GATGCTAAATGGAGCAATGGTGACAAAGTGACTGCGCAAGATTTTGTTTATGCTTGGAGA
    AAAACAGTTGACCCTAAAACAGGTTCTGAATTTGCATACATTATGGGGGACATTAAAAAT
    GCGAGTGATATTAGTACTGGTAAGAAACCTGTAGAGCAATTAGGTATCAAAGCATTAGAT
    GATGAAACATTACAAATTGATTTAGAAAAGCCGGTTCCATATATTAATCAATTATTAGCA
    CTTAATACCTTTGCACCTCAAAATGAAAAAGTTGCCAAAAAATATGGTAAAAATTACGGT
    ACGGCAGCTGATAGAGCGGTATACAATGGTCCATTTAAAGTTGATGATTGGAAACAAGAA
    GATAAAACCTTACTATCTAAAAATCAGTATTATTGGGATAAAAAGAATGTAAAATTAGAT
    AAAGTGAATTATAAAGTTATTAAAGACTTACAAGCCGGTGCATCATTGTATGATACTGAA
    TCAGTAGATGACGCAGTTATTACTGCAGATCAAGTAAATAAATATAAAGATAACAAAGGA
    TTAAACTTTGTGTTAACGACTGGGACATTTTTTGTAAAAATGAATGAAAAACAATATCCT
    GATTTTAAAAACAAAAATTTAAGACTGGCTATCGCACAAGCAATAGATAAAAAAGGATAC
    GTTGATTCAGTGAAAAACAATGGCTCAATTCCTTCCGATACACTAACAGCCAAAGGAATT
    GCGAAAGCGCCTAATGGCAAAGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAAT
    CCTAAAGAAGCAAGAGCACACTGGGACAAAGCTAAAAAAGAGTTAGGTAAAAATGAAGTG
    ACATTTTCAATGAACACAGAAGATACACCAGATGCAAAAATATCTGCTGAATATATCAAA
    TCGCAAGTTGAGAAAAATTTACCAGGAGTTACTTTGAAAATTAAGCAATTACCGTTTAAA
    CAAAGAGTATCATTAGAACTGAGTAACAATTTTGAAGCATCACTTAGTGGTTGGTCTGCA
    GATTACCCTGATCCTATGGCTTATTTAGAAACAATGACCACAGGTAGCGCACAAAATAAT
    ACAGACTGGGGTAATAAAGAATATGATCAATTACTTAAAGTAGCAAGAACCAAATTGGCA
    CTTCAACCGAACGAACGATATGAAAACTTGAAAAAAGCAGAAGAAATGTTCCTAGGAGAT
    GCACCGGTAGCACCAATTTATCAAAAAGGTGTTGCACATTTAACAAATCCTCAAGTAAAA
    GGATTAATTTACCATAAATTTGGTCCAAATAACTCACTTAAACATGTATATATTGATAAA
    TCGATAGATAAAGAAACAGGTAAGAAGAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1656

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000950
  • symbol: JSNZ_000950
  • description: peptide ABC transporter substrate-binding protein
  • length: 551
  • theoretical pI: 9.72302
  • theoretical MW: 61500.5
  • GRAVY: -0.636298

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 64.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 271.9)
    and 1 more
    no clan defined DUF7660; Family of unknown function (DUF7660) (PF24693; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 5.13
    • Extracellular Score: 4.87
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0003
    • Cytoplasmic Membrane Score: 0.8661
    • Cell wall & surface Score: 0.0434
    • Extracellular Score: 0.0902
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.000807
    • TAT(Tat/SPI): 0.000113
    • LIPO(Sec/SPII): 0.998667
    • Cleavage Site: CS pos: 20-21. LSG-CA. Pr: 0.9996
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKGGKTLKVKLRSDAKWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALDDETLQIDLEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J Bacteriol: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)

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