⊟Summary[edit | edit source]

pan ID^?: SAUPAN003137000
symbol^?: opp3A
synonym:
description^?: peptide ABC transporter substrate-binding protein
- peptide ABC transporter substrate-binding protein
- oligopeptide ABC transporter substrate-binding protein
- oligopeptide ABC transporter, substrate-binding lipoprotein oppA, putative
- oligopeptide ABC transporter oligopeptide-binding protein
- oligopeptide ABC superfamily ATP binding cassette transporter, binding protein
- Oligopeptide-binding protein
- transport system extracellular bindinglipoprotein
- 4-phytase
- extracellular solute-binding proteins, family 5 Middle family protein
- oligopeptide ABC superfamily ATP binding cassette transporter, binding protein, Opp3A
- oligopeptide ABC transporter, oligopeptide-binding protein
- Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein oppA
- oligopeptide ABC transporter substrate-binding protein OppA
- peptide binding protein OppA
- pseudogene
strand^?: +
coordinates^?: 3520594..3522249
synteny block^?: BlockID0022290
occurrence^?: in 100% of 34 strains

opp3A : oligopeptide ABC transporter substrate-binding protein A ^[1]

The oligopeptide permease 3 (opp-3) system is the primary mechanism for staphylococcal uptake of amino acid polymers from 3 to 8 residues from the environment for nutrition. Its expression appears to be induced by tyrosine, although this induction can be competitively inhibited by phenylalanine. Intriguingly, deactivation of the opp-3 system can reduce expression of the staphylococcal extracellular proteases that ultimately provide the oligopeptide substrates for opp-3 transport.

⊟Orthologs[edit | edit source]

COL:

N315:

NCTC8325:

Newman:

USA300_FPR3757:

SAUSA300_0891 (oppA)

04-02981:

SA2981_0945 (oppA)

08BA02176:

C248_1013

11819-97:

MS7_0945

6850:

RSAU_000864 (oppA1)

71193:

ST398NM01_0986

ECT-R 2:

ECTR2_845

ED133:

SAOV_0936

ED98:

SAAV_0951

HO 5096 0412:

SAEMRSA15_08190

JH1:

SaurJH1_1008

JH9:

SaurJH9_0989

JKD6008:

SAA6008_00943 (oppA)

JKD6159:

SAA6159_00847 (opp3A)

JSNZ:

JSNZ_000950

LGA251:

SARLGA251_09060

M013:

M013TW_0912

MRSA252:

SAR0953

MSHR1132:

SAMSHR1132_08380

MSSA476:

SAS0860

Mu3:

SAHV_0985

Mu50:

SAV0990

MW2:

MW0872

RF122:

SAB0856

ST398:

SAPIG0986

T0131:

SAT0131_01023

TCH60:

HMPREF0772_12255 (oppA)

TW20:

SATW20_09880

USA300_TCH1516:

USA300HOU_0948 (oppA1)

VC40:

SAVC_04120

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]

alignment of orthologues:
CLUSTAL format alignment by MAFFT L-INS-i (v7.307)

COL             MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVT
N315            MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVT
NCTC8325        MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVT
Newman          MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVT
USA300_FPR3757  MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVT
                ************************************************************

COL             AQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWR
N315            AQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWR
NCTC8325        AQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWR
Newman          AQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWR
USA300_FPR3757  AQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWR
                ************************************************************

COL             KTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALNDETLQIELEKPVPYINQLLA
N315            KTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALNDETLQIELEKPVPYINQLLA
NCTC8325        KTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALNDETLQIELEKPVPYINQLLA
Newman          KTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALNDETLQIELEKPVPYINQLLA
USA300_FPR3757  KTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALNDETLQIELEKPVPYINQLLA
                ************************************************************

COL             LNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLD
N315            LNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLD
NCTC8325        LNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLD
Newman          LNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLD
USA300_FPR3757  LNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLD
                ************************************************************

COL             KVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTTGTFFVKMNEKQYP
N315            KVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTSGTFFVKMNEKQYP
NCTC8325        KVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTTGTFFVKMNEKQYP
Newman          KVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTTGTFFVKMNEKQYP
USA300_FPR3757  KVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTTGTFFVKMNEKQYP
                **********************************************:*************

COL             DFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYN
N315            DFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYN
NCTC8325        DFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYN
Newman          DFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYN
USA300_FPR3757  DFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYN
                ************************************************************

COL             PKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKSQVEKNLPGVTLKIKQLPFK
N315            PKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKSQVEKNLPGVTLKIKQLPFK
NCTC8325        PKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKSQVEKNLPGVTLKIKQLPFK
Newman          PKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKSQVEKNLPGVTLKIKQLPFK
USA300_FPR3757  PKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKSQVEKNLPGVTLKIKQLPFK
                ************************************************************

COL             QRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLA
N315            QRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLA
NCTC8325        QRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLA
Newman          QRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLA
USA300_FPR3757  QRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLA
                ************************************************************

COL             LQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDK
N315            LQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDK
NCTC8325        LQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDK
Newman          LQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDK
USA300_FPR3757  LQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDK
                ************************************************************

COL             SIDKETGKKKK
N315            SIDKETGKKKK
NCTC8325        SIDKETGKKKK
Newman          SIDKETGKKKK
USA300_FPR3757  SIDKETGKKKK
                ***********

↑ Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J Bacteriol: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)Elise Borezée-Durant, Aurelia Hiron, Jean-Christophe Piard, Vincent Juillard
Dual role of the oligopeptide permease Opp3 during growth of Staphylococcus aureus in milk.
Appl Environ Microbiol: 2009, 75(10);3355-7
[PubMed:19286789] [WorldCat.org] [DOI] (I p)McKenzie K Lehman, Austin S Nuxoll, Kelsey J Yamada, Tammy Kielian, Steven D Carson, Paul D Fey
Protease-Mediated Growth of Staphylococcus aureus on Host Proteins Is opp3 Dependent.
mBio: 2019, 10(2);
[PubMed:31040245] [WorldCat.org] [DOI] (I e) Imran Khan, Sandy J MacDonald, Sigurbjorn Markusson et al. Distinct roles for the Staphylococcus aureus POT transporter DtpT in di/tripeptide uptake and glutathione utilisation inside human macrophages. Epub ahead of print. BioRxiv. doi: https://doi.org/10.1101/2024.12.02.626324

[1] Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J Bacteriol: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)Elise Borezée-Durant, Aurelia Hiron, Jean-Christophe Piard, Vincent Juillard
Dual role of the oligopeptide permease Opp3 during growth of Staphylococcus aureus in milk.
Appl Environ Microbiol: 2009, 75(10);3355-7
[PubMed:19286789] [WorldCat.org] [DOI] (I p)McKenzie K Lehman, Austin S Nuxoll, Kelsey J Yamada, Tammy Kielian, Steven D Carson, Paul D Fey
Protease-Mediated Growth of Staphylococcus aureus on Host Proteins Is opp3 Dependent.
mBio: 2019, 10(2);
[PubMed:31040245] [WorldCat.org] [DOI] (I e) Imran Khan, Sandy J MacDonald, Sigurbjorn Markusson et al. Distinct roles for the Staphylococcus aureus POT transporter DtpT in di/tripeptide uptake and glutathione utilisation inside human macrophages. Epub ahead of print. BioRxiv. doi: https://doi.org/10.1101/2024.12.02.626324

[1]

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Contents

⊟Summary[edit | edit source]

⊟Orthologs[edit | edit source]

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757

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⊟Summary[edit | edit source]

⊟Orthologs[edit | edit source]

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]