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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02049
- pan locus tag?: SAUPAN001675000
- symbol: SAOUHSC_02049
- pan gene symbol?: —
- synonym:
- product: PBSX family phage terminase large subunit
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_02049
- symbol: SAOUHSC_02049
- product: PBSX family phage terminase large subunit
- replicon: chromosome
- strand: -
- coordinates: 1948782..1950059
- length: 1278
- essential: no DEG
⊟Accession numbers[edit | edit source]
- Gene ID: 3920499 NCBI
- RefSeq: YP_500542 NCBI
- BioCyc: G1I0R-1939 BioCyc
- MicrobesOnline: 1290498 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1261ATGACGAAAGTTAAATTAAACTTTAACAAACCGTCTAATGTTTTCAATAGAAACATATTC
GAAATACTAACCAATTACGATAACTTCACTGAAGTACATTACGGTGGAGGTTCGAGCGGT
AAGTCTCACGGCGTTATACAAAAAGTTGTACTCAAAGCATTGCAAGATTGGAAATATCCT
AGGCGTATACTGTGGCTTAGAAAAGTACAATCAACAATTAAAGATAGTTTGTTCGAAGAT
GTTAAAGATTGTTTGATAAACTTTGGTATTTGGGACATGTGCCTTTGGAATAAGACTGAT
AACAAAGTTGAATTGCCAAACGGCGCAGTTTTTTTGTTTAAAGGATTAGATAACCCAGAG
AAAATAAAGTCGATAAAAGGCATATCAGACATAGTCATGGAAGAAGCGTCTGAATTCACA
CTAAATGATTACACGCAATTAACGTTGCGTTTGAGGGAGCGTAAACACGTGAATAAGCAA
ATATTTTTGATGTTTAACCCAGTATCTAAACTGAATTGGGTTTATAAGTATTTCTTTGAA
CATGGTGAACCAATGGAAAATGTCATGATTAGACAATCTAGTTATCGAGATAATAAGTTT
CTTGATGAAATGACACGACAAAACTTAGAGTTGTTAGCAAATCGTAATCCAGCATATTAC
AAAATTTATGCGTTAGGTGAATTTGCTACACTAGACAAATTGGTTTTCCCTAAGTATGAA
AAACGTTTAATAAATAAAGATGAGTTAAGACATTTACCTTCTTATTTTGGATTGGACTTT
GGCTACGTTAATGATCCTAGTGCTTTTATACATTCTAAAATAGATGTAAAGAAAAAGAAG
TTATACATCATTGAAGAGTATGTTAAACAAGGTATGCTGAATGATGAAATAGCTAATGTC
ATAAAGCAACTTGGTTATGCTAAAGAAGAAATTACAGCAGATAGTGCAGAACAAAAAAGT
ATAGCTGAATTAAGGAATCTAGGGCTTAAAAGGATTTTACCAACCAAAAAAGGGAAGGGC
TCGGTTGTACAAGGGTTACAATTCTTAATGCAATTTGAAATCATTGTTGATGAACGTTGT
TTCAAGACTATTGAAGAGTTTGACAACTACACATGGCAAAAGGACAAAGATACAGGTGAA
TATACCAATGAACCAGTAGATACATACAATCATTGTATCGATTCGTTGCGTTATTCAGTG
GAACGATTCTACAGACCGGTTAGAAAACGCACAAATCTCAGTTCGAAAGTTGACACAATA
AAATCTCTAGGATTATAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02049
- symbol: SAOUHSC_02049
- description: PBSX family phage terminase large subunit
- length: 425
- theoretical pI: 9.34345
- theoretical MW: 49926.1
- GRAVY: -0.513647
⊟Function[edit | edit source]
- TIGRFAM: Mobile and extrachromosomal element functions Prophage functions phage terminase, large subunit, PBSX family (TIGR01547; HMM-score: 394.3)
- TheSEED :
- Phage terminase, large subunit
- PFAM: P-loop_NTPase (CL0023) Terminase_3; Phage terminase large subunit (PF04466; HMM-score: 203.6)and 4 moreRNase_H (CL0219) Terminase_3C; Terminase RNAseH like domain (PF17288; HMM-score: 119.3)P-loop_NTPase (CL0023) Terminase_6; Terminase-like family (PF03237; HMM-score: 29.9)RNase_H (CL0219) Terminase_6C; Terminase RNaseH-like domain (PF17289; HMM-score: 16.2)UBA (CL0214) UBA_3; Fungal ubiquitin-associated domain (PF09288; HMM-score: 12.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005572
- TAT(Tat/SPI): 0.000246
- LIPO(Sec/SPII): 0.000493
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTKVKLNFNKPSNVFNRNIFEILTNYDNFTEVHYGGGSSGKSHGVIQKVVLKALQDWKYPRRILWLRKVQSTIKDSLFEDVKDCLINFGIWDMCLWNKTDNKVELPNGAVFLFKGLDNPEKIKSIKGISDIVMEEASEFTLNDYTQLTLRLRERKHVNKQIFLMFNPVSKLNWVYKYFFEHGEPMENVMIRQSSYRDNKFLDEMTRQNLELLANRNPAYYKIYALGEFATLDKLVFPKYEKRLINKDELRHLPSYFGLDFGYVNDPSAFIHSKIDVKKKKLYIIEEYVKQGMLNDEIANVIKQLGYAKEEITADSAEQKSIAELRNLGLKRILPTKKGKGSVVQGLQFLMQFEIIVDERCFKTIEEFDNYTWQKDKDTGEYTNEPVDTYNHCIDSLRYSVERFYRPVRKRTNLSSKVDTIKSLGL
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_02047 < SAOUHSC_02048 < SAOUHSC_02049 < SAOUHSC_02050predicted SigA promoter [1] : S810 < SAOUHSC_02019 < SAOUHSC_02020 < SAOUHSC_02021 < SAOUHSC_02022 < SAOUHSC_02023 < SAOUHSC_02025 < SAOUHSC_02026 < SAOUHSC_02027 < SAOUHSC_02028 < SAOUHSC_02029 < SAOUHSC_02030 < SAOUHSC_02031 < SAOUHSC_02033 < SAOUHSC_02034 < SAOUHSC_02035 < SAOUHSC_02036 < SAOUHSC_02037 < SAOUHSC_02038 < SAOUHSC_02040 < SAOUHSC_02041 < SAOUHSC_02042 < SAOUHSC_02043 < SAOUHSC_02044 < S811 < SAOUHSC_02046 < SAOUHSC_02047 < SAOUHSC_02048 < SAOUHSC_02049 < SAOUHSC_02050 < S812 < SAOUHSC_02051 < SAOUHSC_02052 < SAOUHSC_02053 < SAOUHSC_02054 < SAOUHSC_02055 < SAOUHSC_02056 < SAOUHSC_02057 < SAOUHSC_02058 < SAOUHSC_02059 < SAOUHSC_02060 < SAOUHSC_02061 < SAOUHSC_02062 < SAOUHSC_02063 < SAOUHSC_02064 < SAOUHSC_02065 < SAOUHSC_02066 < SAOUHSC_02067 < SAOUHSC_02068 < SAOUHSC_02069 < SAOUHSC_02070 < SAOUHSC_02071 < S813 < SAOUHSC_02072 < SAOUHSC_02073 < SAOUHSC_02074 < SAOUHSC_02075 < SAOUHSC_02076 < SAOUHSC_02077 < SAOUHSC_02078 < SAOUHSC_02079 < SAOUHSC_02080 < S816 < SAOUHSC_02081 < SAOUHSC_02083
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [1]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)