PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1015 [new locus tag: NWMN_RS05750 ]
• pan locus tag^?: SAUPAN001675000
• symbol: NWMN_1015
• pan gene symbol^?: —
• synonym:
• product: phage terminase large subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1015 [new locus tag: NWMN_RS05750 ]
• symbol: NWMN_1015
• product: phage terminase large subunit
• replicon: chromosome
• strand: +
• coordinates: 1115916..1117193
• length: 1278
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330619 NCBI
• RefSeq: YP_001332049 NCBI
• BioCyc:
• MicrobesOnline: 3706566 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGACGAAAGTTAAATTAAACTTTAACAAACCATCTAATGTTTTCAATAGAAACATATTC
  GAAATACTAACCAATTACGATAACTTCACTGAAGTACATTACGGTGGAGGTTCGAGCGGT
  AAGTCTCACGGCGTTATACAAAAAGTTGTACTTAAAGCATTGCAAGACTGGAAATATCCT
  AGGCGTATACTATGGCTTAGAAAAGTCCAATCAACAATTAAAGATAGTTTATTCGAAGAT
  GTCAAAGATTGTTTGATAAACTTCGGTATTTGGGACATGTGCCTTTGGAATAAGACTGAT
  AACAAAGTTGAATTGCCAAACGGCGCAGTTTTTTTGTTTAAAGGATTAGATAACCCAGAG
  AAAATAAAGTCGATAAAAGGCATATCAGACATAGTCATGGAAGAAGCGTCTGAATTCACA
  CTAAATGATTACACGCAATTAACGTTGCGTTTGAGGGAGCGTAAACACGTGAATAAGCAA
  ATATTTTTGATGTTTAACCCAGTATCTAAACTGAATTGGGTTTATAAGTATTTCTTTGAA
  CATGGTGAACCAATGGAAAATGTCATGATTAGACAATCTAGTTATCGAGATAATAAGTTT
  CTTGATGAAATGACACGACAAAACTTAGAGTTGTTAGCAAATCGTAATCCAGCATATTAC
  AAAATTTATGCGTTAGGTGAATTTGCTACACTAGACAAATTGGTTTTCCCTAAGTATGAA
  AAACGTTTAATAAATAAAGATGAGTTAAGACATTTACCTTCTTATTTTGGATTGGACTTT
  GGCTACGTTAATGATCCTAGTGCTTTTATACATTCTAAAATAGATGTAAAGAAAAAGAAG
  TTATACATCATTGAAGAGTATGTTAAACAAGGTATGCTGAATGATGAAATAGCTAATGTC
  ATAAAGCAACTTGGTTATGCTAAAGAAGAAATTACAGCAGATAGTGCAGAACAAAAAAGT
  ATAGCTGAATTAAGGAATCTAGGGCTTAAAAGGATTTTACCAACCAAAAAAGGGAAGGGC
  TCGGTTGTACAAGGGTTACAATTCTTAATGCAATTTGAAATCATTGTTGATGAACGTTGT
  TTCAAGACTATTGAAGAGTTTGACAACTACACATGGCAAAAGGACAAAGATACAGGTGAA
  TATACCAATGAACCAGTAGATACATACAATCATTGTATCGATTCGTTGCGTTATTCAGTG
  GAACGATTCTACAGACCGGTTAGAAAACGCACAAATGTCAGTTCGAAAGTTGACACAATA
  AAATCTCTAGGATTATAG
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⊟Protein

⊟General

• locus tag: NWMN_1015 [new locus tag: NWMN_RS05750 ]
• symbol: NWMN_1015
• description: phage terminase large subunit
• length: 425
• theoretical pI: 9.34345
• theoretical MW: 49912
• GRAVY: -0.512706

⊟Function

• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage terminase, large subunit, PBSX family (TIGR01547; HMM-score: 394.5)
• TheSEED: data available for NCTC8325
• PFAM:
  P-loop_NTPase (CL0023) Terminase_3; Phage terminase large subunit (PF04466; HMM-score: 203.6)
  and 4 more
  RNase_H (CL0219) Terminase_3C; Terminase RNAseH like domain (PF17288; HMM-score: 119.3)
  P-loop_NTPase (CL0023) Terminase_6; Terminase-like family (PF03237; HMM-score: 29.9)
  RNase_H (CL0219) Terminase_6C; Terminase RNaseH-like domain (PF17289; HMM-score: 16.2)
  UBA (CL0214) UBA_3; Fungal ubiquitin-associated domain (PF09288; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005572
  • TAT(Tat/SPI): 0.000246
  • LIPO(Sec/SPII): 0.000493
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221227 NCBI
• RefSeq: YP_001332049 NCBI
• UniProt: A0A0H3KC71 UniProt

⊟Protein sequence

• MTKVKLNFNKPSNVFNRNIFEILTNYDNFTEVHYGGGSSGKSHGVIQKVVLKALQDWKYPRRILWLRKVQSTIKDSLFEDVKDCLINFGIWDMCLWNKTDNKVELPNGAVFLFKGLDNPEKIKSIKGISDIVMEEASEFTLNDYTQLTLRLRERKHVNKQIFLMFNPVSKLNWVYKYFFEHGEPMENVMIRQSSYRDNKFLDEMTRQNLELLANRNPAYYKIYALGEFATLDKLVFPKYEKRLINKDELRHLPSYFGLDFGYVNDPSAFIHSKIDVKKKKLYIIEEYVKQGMLNDEIANVIKQLGYAKEEITADSAEQKSIAELRNLGLKRILPTKKGKGSVVQGLQFLMQFEIIVDERCFKTIEEFDNYTWQKDKDTGEYTNEPVDTYNHCIDSLRYSVERFYRPVRKRTNVSSKVDTIKSLGL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1014 > NWMN_1015 > NWMN_1016 > NWMN_1017

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications