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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02019
- pan locus tag?:
- symbol: SAOUHSC_02019
- pan gene symbol?: —
- synonym:
- product: autolysin
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_02019
- symbol: SAOUHSC_02019
- product: autolysin
- replicon: chromosome
- strand: -
- coordinates: 1924647..1926092
- length: 1446
- essential: unknown
⊟Accession numbers[edit | edit source]
- Gene ID: 3920473 NCBI
- RefSeq: YP_500516 NCBI
- BioCyc: G1I0R-1913 BioCyc
- MicrobesOnline: 1290472 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1441ATGCAAGCAAAATTAACTAAAAATGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAA
CAATTCAATGTGGACTTATGGTATGGATTTCAATGCTTTGATTATGCCAATGCTGGTTGG
AAAGTTTTGTTTGGATTACTTCTAAAAGGTTTAGGTGCAAAAGATATTCCGTTCGCTAAC
AACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGACTTCTTAGCACAACCTGGC
GACATGGTGGTATTCGGTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATT
GAAGCAACTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACT
GACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTAT
GATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATCAGTT
CAATCTCCTACACAAGCACCTAAAAAAGAAACAGCTAAGCCACAACCTAAAGCAGTAGAA
CTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCTAAGCGTGGTAGTAACCCT
AAAGGTATAGTTATACACAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGT
AACGGATTAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTA
TCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGCATACCGCTAAT
CAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGATAAC
GCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGCGCTAGATTGTTGAAAAAA
TGGGGATTACCAGCAAACAGAAATACAATCAGATTGCACAATGAATTTACTTCAACATCA
TGCCCTCATAGAAGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTG
CCAGAAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGTACATG
GATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTT
AAACCAGTTGCAAGTGCATGGAAACGTAATAAATATGGTACTTACTACATGGAAGAAAGT
GCTAGATTCACAAACGGCAATCAACCAATCACAGTAAGAAAAGTGGGGCCATTCTTATCT
TGTCCAGTGGGTTATCAGTTCCAACCTGGTGGGTATTGTGATTATACAGAAGTGATGTTA
CAAGATGGTCATGTTTGGGTAGGATATACATGGGAGGGGCAACGTTATTACTTGCCTATT
AGAACATGGAATGGTTCTGCCCCACCTAATCAGATATTAGGTGACTTATGGGGAGAAATC
AGTTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02019
- symbol: SAOUHSC_02019
- description: autolysin
- length: 481
- theoretical pI: 8.64154
- theoretical MW: 54029.6
- GRAVY: -0.46632
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED :
- Phage lysin, N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
- PFAM: SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 85.8)and 3 moreno clan defined Amidase_2; N-acetylmuramoyl-L-alanine amidase (PF01510; HMM-score: 57.7)Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 46.6)HTH (CL0123) Myb_DNA-bind_2; Rap1 Myb domain (PF08914; HMM-score: 12.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0.1
- Cytoplasmic Membrane Score: 0.14
- Cellwall Score: 0.15
- Extracellular Score: 9.6
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.024253
- TAT(Tat/SPI): 0.000407
- LIPO(Sec/SPII): 0.000866
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MQAKLTKNEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSVQSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKYGTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPIRTWNGSAPPNQILGDLWGEIS
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_02019 < SAOUHSC_02020predicted SigA promoter [3] : S810 < SAOUHSC_02019 < SAOUHSC_02020 < SAOUHSC_02021 < SAOUHSC_02022 < SAOUHSC_02023 < SAOUHSC_02025 < SAOUHSC_02026 < SAOUHSC_02027 < SAOUHSC_02028 < SAOUHSC_02029 < SAOUHSC_02030 < SAOUHSC_02031 < SAOUHSC_02033 < SAOUHSC_02034 < SAOUHSC_02035 < SAOUHSC_02036 < SAOUHSC_02037 < SAOUHSC_02038 < SAOUHSC_02040 < SAOUHSC_02041 < SAOUHSC_02042 < SAOUHSC_02043 < SAOUHSC_02044 < S811 < SAOUHSC_02046 < SAOUHSC_02047 < SAOUHSC_02048 < SAOUHSC_02049 < SAOUHSC_02050 < S812 < SAOUHSC_02051 < SAOUHSC_02052 < SAOUHSC_02053 < SAOUHSC_02054 < SAOUHSC_02055 < SAOUHSC_02056 < SAOUHSC_02057 < SAOUHSC_02058 < SAOUHSC_02059 < SAOUHSC_02060 < SAOUHSC_02061 < SAOUHSC_02062 < SAOUHSC_02063 < SAOUHSC_02064 < SAOUHSC_02065 < SAOUHSC_02066 < SAOUHSC_02067 < SAOUHSC_02068 < SAOUHSC_02069 < SAOUHSC_02070 < SAOUHSC_02071 < S813 < SAOUHSC_02072 < SAOUHSC_02073 < SAOUHSC_02074 < SAOUHSC_02075 < SAOUHSC_02076 < SAOUHSC_02077 < SAOUHSC_02078 < SAOUHSC_02079 < SAOUHSC_02080 < S816 < SAOUHSC_02081 < SAOUHSC_02083
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)