PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary

• organism: Staphylococcus aureus NCTC8325
• locus tag: SAOUHSC_02023
• pan locus tag^?: SAUPAN001702000
• symbol: SAOUHSC_02023
• pan gene symbol^?: —
• synonym:
• product: bifunctional autolysin

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAOUHSC_02023
• symbol: SAOUHSC_02023
• product: bifunctional autolysin
• replicon: chromosome
• strand: -
• coordinates: 1928152..1930050
• length: 1899
• essential: no DEG

⊟Accession numbers

• Gene ID: 3920477 NCBI
• RefSeq: YP_500520 NCBI
• BioCyc: G1I0R-1917 BioCyc
• MicrobesOnline: 1290476 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGGATTACCTAATCCGAAAAATAGAAAGCCCACAGCTAGTGAAGTGGTTGAATGGGCG
  TTATATATCGCTAAAAACAAAATAGCTATTGATGTACCTGGTTCTGGAATGGGAGCACAA
  TGCTGGGATTTACCTAATTATTTACTCGATAAATATTGGGGGTTTAGAACATGGGGAAAT
  GCTGATGCTATGGCTCAAAAATCCAATTATAGAGGTAGAGATTTCAAGATAATTAGAAAT
  ACAAAAGATTTTGTACCACAACCAGGCGACTGGGGTGTTTGGACTGGTGGTTGGGCAGGA
  CATGTAAACATTGTAGTGGGACCATGCACAAAAGACTATTGGTATGGCGTAGATCAAAAC
  TGGTATACAAATAACGCAACAGGAAGTCCACCTTATAAAATTAAACACTCTTATCATGAT
  GGACCAGGTGGAGGGGTTAAATATTTTGTTAGACCACCATATCATCCAGACAAAACTACA
  CCGGCACCTAAACCAGAAGATGATAGTGATGATAACGAAAAAAATAATAAAAAAGTTCCA
  ATTTGGAAAGATGTAACAACTATAAAGTACACTATTTCTAGCCAAGAGGTTAATTATCCA
  GAATATATTTATCACTTTATAGTAGAAGGTAATCGACGACTCGAAAAACCTAAAGGAATA
  ATGATTAGAAACGCACAAACGATGAGCTCGGTAGAAAGTTTATATAACAGTAGGAAGAAA
  TACAAACAGGATGTAGAATATCCCCACTTTTATGTTGATAGACATAATATTTGGGCACCT
  AGAAGAGCTGTATTTGAAGTTCCTAATGAACCTGATTATATAGTTATAGACGTATGTGAA
  GATTATAGTGCGAGTAAAAATGAATTTATTTTTAATGAGATTCACGCAATGGTTGTAGCT
  GTAGATATGATGGCCAAATATGAGATACCTCTAAGTATTGAAAATTTAAAAGTAGACGAC
  AGCATTTGGCGTTCAATGTTGGAACATGTTAATTGGAATATGATTGACAACGGTGTTCCT
  CCTAAAGATAAATACGAAGCATTAGAAAAGGCATTACTTAATATATTTAAAAACAGAGAA
  AAATTATTAAATTCTATAACTAAGCCAACAGTAACAAAATCTAGAATAAAAGTTATGGTA
  GATAATAAAAACGCTGATATAGCTAATGTAAGAGACTCGTCACCAACAGCCAACAATGGT
  TCGGCATCTAAACAACCGCAGATCATAACAGAAACGAGTCCTTATACATTCAAACAAGCA
  CTGGATAAACAAATGGCAAGAGGTAACCCGAAAAAATCTAATGCTTGGGGTTGGGCTAAC
  GCTACACGAGCACAAACGAGTTCAGCAATGAATGTAAAGCGTATATGGGAAAGTAACACA
  CAATGCTACCAAATGCTTAATTTAGGCAAGTATCAAGGTGTTTCAGTTAGCGCACTTAAT
  AAGATACTTAAAGGTAAGGGAACATTGAATAATCAAGGTAAAGCGTTCGCAGAAGCTTGT
  AAAAAGCACAACATTAATGAAATTTATTTAATCGCGCATGCTTTCTTAGAAAGTGGATAT
  GGAACAAGTAACTTCGCTAACGGAAAAGATGGAGTATACAACTACTTCGGCATTGGCGCT
  TACGACAACAATCCTAACTACGCAATGACGTTTGCAAGGAATAAAGGTTGGACATCTCCA
  GCAAAAGCAATCATGGGCGGTGCTAGCTTCGTAAGAAAGGATTACATCAATAAAGGTCAA
  AACACATTGTACCGAATTAGATGGAATCCTAAGAATCCAGCTACCCACCAATACGCTACT
  GCTATAGAGTGGTGCCAACATCAAGCAAGTACAATCGCTAAGTTATATAAACAAATCGGC
  TTAAAAGGTATCTACTTCACAAGGGATAAATATAAATAA
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⊟Protein

⊟General

• locus tag: SAOUHSC_02023
• symbol: SAOUHSC_02023
• description: bifunctional autolysin
• length: 632
• theoretical pI: 9.77316
• theoretical MW: 72002.1
• GRAVY: -0.688608

⊟Function

• reaction:
  EC 3.5.1.28?  ExPASy
  N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
• TIGRFAM:
  flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 22.1)
• TheSEED  :
  • Phage endo-beta-N-acetylglucosaminidase (EC 3.2.1.96)
  • Phage endolysin N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory Murein Hydrolases  N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
  and 3 more
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory Recycling of Peptidoglycan Amino Acids  N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
  Regulation and Cell signaling Quorum sensing and biofilm formation Biofilm formation in Staphylococcus  Bifunctional autolysin Atl
  Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Bifunctional autolysin Atl
• PFAM:
  Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 50.7)
  and 3 more
  Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 29.9)
  Lysozyme (CL0037) SLT_2; Transglycosylase SLT domain (PF13406; HMM-score: 12.9)
  no clan defined INCENP_ARK-bind; Inner centromere protein, ARK binding region (PF03941; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.72
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.031405
  • TAT(Tat/SPI): 0.001237
  • LIPO(Sec/SPII): 0.002792
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 88195711 NCBI
• RefSeq: YP_500520 NCBI
• UniProt: Q2FX73 UniProt
• STRING: 93061.SAOUHSC_02023 STRING

⊟Protein sequence

• MGLPNPKNRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDLPNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHVNIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPDKTTPAPKPEDDSDDNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPKGIMIRNAQTMSSVESLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVIDVCEDYSASKNEFIFNEIHAMVVAVDMMAKYEIPLSIENLKVDDSIWRSMLEHVNWNMIDNGVPPKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRDSSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTSSAMNVKRIWESNTQCYQMLNLGKYQGVSVSALNKILKGKGTLNNQGKAFAEACKKHNINEIYLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMGGASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGIYFTRDKYK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAOUHSC_02022 < SAOUHSC_02023
  predicted SigA promoter  : S810 < SAOUHSC_02019 < SAOUHSC_02020 < SAOUHSC_02021 < SAOUHSC_02022 < SAOUHSC_02023 < SAOUHSC_02025 < SAOUHSC_02026 < SAOUHSC_02027 < SAOUHSC_02028 < SAOUHSC_02029 < SAOUHSC_02030 < SAOUHSC_02031 < SAOUHSC_02033 < SAOUHSC_02034 < SAOUHSC_02035 < SAOUHSC_02036 < SAOUHSC_02037 < SAOUHSC_02038 < SAOUHSC_02040 < SAOUHSC_02041 < SAOUHSC_02042 < SAOUHSC_02043 < SAOUHSC_02044 < S811 < SAOUHSC_02046 < SAOUHSC_02047 < SAOUHSC_02048 < SAOUHSC_02049 < SAOUHSC_02050 < S812 < SAOUHSC_02051 < SAOUHSC_02052 < SAOUHSC_02053 < SAOUHSC_02054 < SAOUHSC_02055 < SAOUHSC_02056 < SAOUHSC_02057 < SAOUHSC_02058 < SAOUHSC_02059 < SAOUHSC_02060 < SAOUHSC_02061 < SAOUHSC_02062 < SAOUHSC_02063 < SAOUHSC_02064 < SAOUHSC_02065 < SAOUHSC_02066 < SAOUHSC_02067 < SAOUHSC_02068 < SAOUHSC_02069 < SAOUHSC_02070 < SAOUHSC_02071 < S813 < SAOUHSC_02072 < SAOUHSC_02073 < SAOUHSC_02074 < SAOUHSC_02075 < SAOUHSC_02076 < SAOUHSC_02077 < SAOUHSC_02078 < SAOUHSC_02079 < SAOUHSC_02080 < S816 < SAOUHSC_02081 < SAOUHSC_02083

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser:   
  
  Multi-gene expression profiles

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications