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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2214 [new locus tag: SAUSA300_RS12215 ]
  • pan locus tag?: SAUPAN005718000
  • symbol: SAUSA300_2214
  • pan gene symbol?: femX
  • synonym:
  • product: FmhB protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2214 [new locus tag: SAUSA300_RS12215 ]
  • symbol: SAUSA300_2214
  • product: FmhB protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2383648..2384913
  • length: 1266
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGAAAAGATGCATATCACTAATCAGGAACATGACGCATTTGTTAAATCCCACCCAAAT
    GGAGATTTATTACAATTAACGAAATGGGCAGAAACAAAGAAATTAACTGGATGGTACGCG
    CGAAGAATCGCTGTAGGTCGTGACGGTGAAGTTCAGGGTGTTGCGCAGTTACTTTTTAAA
    AAAGTACCTAAATTACCTTATACGCTATGTTATATTTCGCGTGGTTTTGTTGTTGATTAT
    AGTAATAAAGAAGCGTTAAATGCATTGTTAGACAGTGCAAAAGAAATTGCTAAAGCTGAG
    AAAGCGTATGCAATTAAAATCGATCCTGATGTTGAAGTTGATAAAGGTACAGATGCTTTG
    CAAAATTTGAAAGCGCTTGGTTTTAAACATAAAGGATTTAAAGAAGGTTTATCAAAAGAC
    TACATCCAACCACGTATGACTATGATTACACCAATTGATAAAAATGATGATGAGTTATTA
    AATAGTTTTGAACGCCGAAATCGTTCAAAAGTGCGCTTGGCTTTAAAGCGAGGTACGACA
    GTAGAACGATCTGATAGAGAAGGTTTAAAAACATTTGCTGAGTTAATGAAAATCACTGGG
    GAACGCGATGGCTTCTTAACGCGTGATATTAGTTACTTTGAAAATATTTATGATGCGTTG
    CATGAAGATGGAGATGCTGAACTATTTTTAGTAAAGTTGGATCCAAAAGAAAATATAGCG
    AAAGTAAATCAAGAATTGAATGAACTTCATGCCGAAATTGCTAAATGGCAGCAGAAGATG
    AAAACATCTGAAAAGCAAGCTAAAAAAGCGCAAAATATGATTAATGATGCGCAAAATAAA
    ATTGCTAAAAATGAAGATTTAAAACGAGACCTAGAAGCTTTAGAAAAGGAACATCCTGAA
    GGTATTTATCTTTCTGGTGCACTATTAATGTTTGCTGGCTCAAAATCATATTACTTATAT
    GGTGCGTCTTCTAATGAATTTAGAGATTTTTTACCAAATCATCATATGCAGTATACGATG
    ATGAAGTATGCACGTGAACATGGTGCAACAACTTACGATTTCGGTGGTACAGATAATGAT
    CCAGATAAAGACTCAGAACATTATGGATTATGGGCATTTAAAAAAGTGTGGGGAACATAC
    TTAAGTGAAAAGATTGGTGAATTTGATTATGTATTGAATCAGCCATTGTACCAATTAATT
    GAGCAAGTTAAACCGCGTTTAACAAAAGCTAAAATTAAAATATCTCGTAAATTAAAACGA
    AAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1266

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2214 [new locus tag: SAUSA300_RS12215 ]
  • symbol: SAUSA300_2214
  • description: FmhB protein
  • length: 421
  • theoretical pI: 9.31875
  • theoretical MW: 48521
  • GRAVY: -0.722803

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.2.16?  ExPASy
    Lipid II:glycine glycyltransferase MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + glycyl-tRNA(Gly) = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + tRNA(Gly)
  • TIGRFAM:
    Unknown function General FemAB-related protein, PEP-CTERM system-associated (TIGR03019; HMM-score: 57.8)
    and 4 more
    SH3 domain protein (TIGR04211; HMM-score: 17.6)
    two transmembrane protein (TIGR04527; HMM-score: 9.7)
    CRISPR type III-A/MTUBE-associated protein Csm2 (TIGR01870; HMM-score: 7.2)
    phage lysis regulatory protein, LysB family (TIGR03495; HMM-score: 5.7)
  • TheSEED  :
    • tRNA-dependent lipid II--glycine ligase (FmhB)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory tRNA-dependent amino acid transfers  tRNA-dependent lipid II--glycine ligase (FmhB)
  • PFAM:
    Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 279.9)
    and 26 more
    Acetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 50.2)
    P-loop_NTPase (CL0023) AAA_13; AAA domain (PF13166; HMM-score: 17.6)
    no clan defined Spc7; Spc7 kinetochore protein (PF08317; HMM-score: 17.4)
    Laminin_II; Laminin Domain II (PF06009; HMM-score: 16.8)
    MIS13; Mis12-Mtw1 protein family (PF08202; HMM-score: 16.3)
    FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 16)
    PDDEXK (CL0236) RmuC; RmuC family (PF02646; HMM-score: 15.4)
    no clan defined IncA; IncA protein (PF04156; HMM-score: 15.4)
    Tropomyosin-lke (CL0452) Tropomyosin_1; Tropomyosin like (PF12718; HMM-score: 13.9)
    no clan defined Med28; Mediator complex subunit 28 (PF11594; HMM-score: 13.4)
    Cep57_CLD_2; Centrosome localisation domain of PPC89 (PF14197; HMM-score: 13.3)
    TMPIT; TMPIT-like protein (PF07851; HMM-score: 13.2)
    FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 12.1)
    no clan defined TMF_TATA_bd; TATA element modulatory factor 1 TATA binding (PF12325; HMM-score: 12.1)
    TRAF_BIRC3_bd; TNF receptor-associated factor BIRC3 binding domain (PF16673; HMM-score: 12.1)
    DUF5069; Domain of unknown function (DUF5069) (PF16798; HMM-score: 12)
    BRE1; BRE1 E3 ubiquitin ligase (PF08647; HMM-score: 11.6)
    DUF3987; Protein of unknown function (DUF3987) (PF13148; HMM-score: 11.1)
    HTH (CL0123) Mnd1; Mnd1 family (PF03962; HMM-score: 10.6)
    no clan defined ISG65-75; Invariant surface glycoprotein (PF11727; HMM-score: 10.2)
    Ax_dynein_light; Axonemal dynein light chain (PF10211; HMM-score: 9.6)
    PCRF; PCRF domain (PF03462; HMM-score: 9)
    XhlA; Haemolysin XhlA (PF10779; HMM-score: 8.6)
    DUF724; Protein of unknown function (DUF724) (PF05266; HMM-score: 8.4)
    DUF1843; Domain of unknown function (DUF1843) (PF08898; HMM-score: 6.7)
    OmpH; Outer membrane protein (OmpH-like) (PF03938; HMM-score: 6.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007329
    • TAT(Tat/SPI): 0.000932
    • LIPO(Sec/SPII): 0.000722
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIAVGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKAYAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELLNSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYDALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMINDAQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNHHMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVLNQPLYQLIEQVKPRLTKAKIKISRKLKRK

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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