PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1518 [new locus tag: SAUSA300_RS08285 ]
• pan locus tag^?: SAUPAN004139000
• symbol: SAUSA300_1518
• pan gene symbol^?: cshB
• synonym:
• product: DEAD-box ATP dependent DNA helicase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1518 [new locus tag: SAUSA300_RS08285 ]
• symbol: SAUSA300_1518
• product: DEAD-box ATP dependent DNA helicase
• replicon: chromosome
• strand: -
• coordinates: 1667925..1669271
• length: 1347
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914764 NCBI
• RefSeq: YP_494213 NCBI
• BioCyc: see SAUSA300_RS08285
• MicrobesOnline: 1293033 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGCAAAACATCCATTCGAACAATTTAATCTAGAATCTAGTTTAATTGACGCTGTGAAA
  GACCTTAATTTTGAAAAACCAACTGAAATTCAGAATCGAATTATTCCAAGAATACTAAAG
  AGAACAAATTTAATTGGTCAATCTCAAACGGGTACAGGGAAATCTCATGCATTTTTATTA
  CCATTAATGCAGTTAATTGATAGTGAAATAAAAGAACCACAAGCAATCGTAGTTGCACCA
  ACAAGAGAACTTGCACAACAACTATACGATGCAGCGAACCATTTAAGCCAATTTAAAGCT
  GGTGTTTCAGTTAAAGTTTTTATTGGTGGTACAGATATAGAGAAAGATAGACAACGTTGT
  AATGCACAACCACAATTGATTATAGGCACCCCTACTAGAATTAATGACTTAGCTAAAACG
  GGACATTTACATGTGCACTTAGCATCATATTTAGTTATTGATGAAGCGGATCTTATGATT
  GACTTAGGATTAATTGAAGATGTAGATTACATTGCTGCAAGATTGGAAGATAATGCAAAT
  ATTGCGGTGTTTAGTGCTACAATCCCACAACAGTTACAACCATTTTTAAATAAATATTTA
  AGTCATCCAGAATATGTAGCTGTCGACAGTAAAAAACAAAATAAAAAGAACATCGAATTC
  TATTTAATACCTACTAAAGGTGCAGCTAAAGTTGAAAAGACTTTAAATTTAATTGATATA
  CTAAATCCATACTTATGTATTATTTTCTGTAATAGTAGAGATAATGCAAATGATTTAGCA
  CGTTCACTAAATGAAGCTGGTATTAAAGTTGGTATGATTCATGGTGGCTTAACGCCACGT
  GAACGTAAACAACAAATGAAACGTATACGTAATTTAGAATTCCAATACGTTATTGCCAGC
  GATTTAGCATCTCGTGGTATTGATATTGAAGGTGTTAGTCATGTCATCAATTTTGATGTG
  CCAAATGATATTGACTTCTTTACGCATAGAGTCGGACGAACTGGTCGTGGGAATTATAAA
  GGTGTAGCAATTACGCTTTATAGTCCTGATGAAGAACACAATATTTCATTAATAGAAGAT
  CGCGGTTTTGTATTCAATACTGTTGATATTAAAGATGGTGAGTTAAAAGAAGTTAAAGCG
  CACAATCAGCGTCAAGCAAGAATGCGCAAAGATGACCATTTAACTAATCAAGTGAAGAAC
  AAAGTTCGAAGTAAAATTAAAAACAAAGTTAAACCAGGTTATAAGAAGAAATTTAAACAA
  GAAGTTGAAAAAATGAAACGTCAAGAGCGTAAGCAATTTAGTAAGCAGCAAAATAGACAA
  AAACGTAAGCAAAACAAAAAAGGTTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1518 [new locus tag: SAUSA300_RS08285 ]
• symbol: SAUSA300_1518
• description: DEAD-box ATP dependent DNA helicase
• length: 448
• theoretical pI: 10.1086
• theoretical MW: 51080.3
• GRAVY: -0.565179

⊟Function

• reaction:
  EC 3.6.4.13?  ExPASy
  RNA helicase ATP + H₂O = ADP + phosphate
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 80.2)
  DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 68.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 67.2)
  and 7 more
  CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 47.3)
  helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 40.4)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 30)
  CRISPR-associated helicase Cas3, subtype CYANO (TIGR03158; HMM-score: 19.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 16.3)
  CRISPR type AFERR-associated DEAD/DEAH-box helicase Csf4 (TIGR03117; HMM-score: 13.9)
  probable extracellular repeat, HAF family (TIGR02913; HMM-score: 12.1)
• TheSEED  :
  • DEAD-box ATP-dependent RNA helicase CshB (EC 3.6.4.13)
  RNA Metabolism RNA processing and modification ATP-dependent RNA helicases, bacterial  DEAD-box ATP-dependent RNA helicase CshB (EC 3.6.4.13)
• PFAM:
  P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 131.7)
  and 2 more
  Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 98.1)
  ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 27)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00254
  • TAT(Tat/SPI): 0.001049
  • LIPO(Sec/SPII): 0.000231
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161365 NCBI
• RefSeq: YP_494213 NCBI
• UniProt: A0A0H2XIF9 UniProt

⊟Protein sequence

• MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNLIGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGVSVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMIDLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNIEFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGGLTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRTGRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDDHLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1517 < SAUSA300_1518

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications