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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0517 [new locus tag: SAUSA300_RS02770 ]
  • pan locus tag?: SAUPAN002300000
  • symbol: SAUSA300_0517
  • pan gene symbol?: —
  • synonym:
  • product: RNA methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0517 [new locus tag: SAUSA300_RS02770 ]
  • symbol: SAUSA300_0517
  • product: RNA methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 578893..579639
  • length: 747
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGGAAGATACGGTTATTGTTGGTAGGCATGCTGTTAGAGAAGCGATTATTACTGGGCAT
    CCGATAAATAAGATATTGATTCAAGAAGGTATTAAAAAGCAACAAATTAATGAAATTTTA
    AAAAATGCAAAAGATCAAAAAATCATTGTTCAAACTGTACCAAAATCTAAATTAGATTTT
    TTAGCAAATGCACCACATCAGGGTGTTGCAGCGCTTATTGCACCATATGAATATGCTGAC
    TTCGATCAATTTTTAAAACAGCAAAAAGAAAAAGAAGGTTTATTGACAGTACTTATATTA
    GACGGCTTAGAAGACCCACATAACTTGGGATCAATTTTAAGAACAGCCGATGCAACGGGA
    GTTGATGGTGTTATTATTCCTAAACGTCGTTCAGTTACACTAACGCAAACAGTTGCAAAA
    GCCTCAACAGGTGCAATTGAACATGTACCAGTTATTCGAGTGACAAATTTAGCTAAAACT
    ATCGATGAACTAAAAGATAATGGCTTTTGGGTAGCTGGCACTGAAGCTAATAATGCAACA
    GATTATAGAAATCTAGAAGCGGACATGTCATTGGCTATTGTAATTGGTAGCGAAGGACAG
    GGTATGAGTCGCCTAGTAAGTGATAAATGCGATTTTTATATTAAGATTCCAATGGTTGGA
    CATGTAAACAGTTTGAATGCTTCGGTTGCAGCAAGTTTAATGATGTACGAAGTATTTCGA
    AAAAGACATGATGTTGGAGAAATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    747

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0517 [new locus tag: SAUSA300_RS02770 ]
  • symbol: SAUSA300_0517
  • description: RNA methyltransferase
  • length: 248
  • theoretical pI: 6.63672
  • theoretical MW: 27207.2
  • GRAVY: -0.0802419

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification RNA methyltransferase, TrmH family, group 3 (TIGR00186; HMM-score: 257.6)
    and 2 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (cytidine(34)-2'-O)-methyltransferase (TIGR00185; EC 2.1.1.207; HMM-score: 46.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification RNA methyltransferase, TrmH family, group 1 (TIGR00050; EC 2.1.1.-; HMM-score: 39.7)
  • TheSEED  :
    • 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-)
    Miscellaneous Plant-Prokaryote comparative genomics Conserved gene cluster possibly involved in RNA metabolism  TrmH family tRNA/rRNA methyltransferase YacO (EC 2.1.1.-)
  • PFAM:
    SPOUT (CL0098) SpoU_methylase; SpoU rRNA Methylase family (PF00588; HMM-score: 140)
    and 2 more
    PELOTA (CL0101) SpoU_sub_bind; RNA 2'-O ribose methyltransferase substrate binding (PF08032; HMM-score: 76.5)
    no clan defined DUF3368; Domain of unknown function (DUF3368) (PF11848; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002276
    • TAT(Tat/SPI): 0.000229
    • LIPO(Sec/SPII): 0.000454
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAKDQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKTIDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPMVGHVNSLNASVAASLMMYEVFRKRHDVGEI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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